Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"DOTAP"”.
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MicroRNA Nanocarriers for the Treatment of Chronic Obstructive Pulmonary Disease
… alcohol) and dioleoyltrimethylammoniumpropane (DOTAP) respectively. The particle size of the anionic NPs was 266.10±20.80 nm and the incorporation of DOTAP resulted in NPs of 244.80±4.40 nm at 15 % DOTAP concentration. The zeta potential (ZP) of 15 % DOTAP NPs was +14.8±0.26 mV. Fluorescently …
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Investigating alternative delivery systems for self-Amplifying RNA vaccines
… - 1, 2-dioleoyl-3-trimethylammonium-propane (DOTAP), or dimethyldioctadecylammonium (DDA) - known as immunostimulants.;In the preliminary studies, two different manufacturing processes such as Microfluidics and Microfluidisation were applied. As a proof of concept, anionic liposomes and solid …
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Design of hybrid retroviral/synthetic gene delivery vectors
… Both chitosan and liposomes composed of DOTAP, DOPE and cholesterol electrostatically associated with M-VLPs forming hybrid vectors that could deliver M-VLPs without the need for the biological envelope protein. The transfection efficiency of these hybrid vectors (χ/M-VLPs and φ/M-VLPs) …
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Interactions of disruptive materials with phospholipid bilayers
… sphingomyelin or cholesterol with a mixed DSPC/DOTAP bilayer. We report that sphingomyelin, but not cholesterol, supports the formation of hexagonal crystalline domains. These domains are small (ca. 70 nm) and can be observed with AFM as high as 40°C. We use differential scanning calorimetry of …
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Quo vadis: quantitative evaluation of intracellular kinetics of siRNA delivered by poly-and-lipoplexes
… methyl-sulfate (DOTAP) liposomes with various amounts of cholesterol as “helper lipids.” In order to gain new insights into the endocytosis of siRNA-based synthetic particles, I exploited the ability of horseradish peroxidase (HRP) to elicit crosslinking and …
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Liposome drug delivery systems for anticancer agents
… positively charged lipid DOTAP, and the zwitterionic phospholipid POPC (8:15:77, mol%). CLS converted from neutrally charged "stealth" liposome to positively charged liposome at tumor interstitual pH owing to the hydrolysis ofPEG<sub>2ooo</sub>-Hz-DHG. The …
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IL -10 gene therapy for the treatment of pulmonary inflammation
… including LipofectAMINE RTM, LipofectinRTM, DOTAP, DEAE-dextran, and the DNA condensing agent protamine sulfate for their ability to promote gene transfection in macrophages. We have developed an efficient transfection protocol which is at least 20--25 fold superior to frequently used …
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Synthesis and conformational study of trans-2-aminocyclohexanol-based pH-triggered molecular switches and their application in gene delivery
… into lipoplexes together with the cationic lipid DOTAP to mediate DNA transfection in Bl6F1 and HeLa cancer cell lines. The lipoplex comprising TACH-lipid 3o (R 1 = C 19 H 37 ; R 2 R 3 = CF 3 CH 2 NH) exhibited one to two orders of magnitude better transfection efficiency than the one with the …
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Design of novel delivery systems to probe alternative routes of administration for a self-amplifying RNA rabies vaccine
… (cLNPs), based on existing cationic lipids (e.g. DOTAP and DDA), was designed to deliver a SAM vaccine. The rabies virus was used as a model, as there is an established correlate of protection (neutralising antibodies) and there exist efficacious vaccines in the market (e.g. Rabipur) to be used as …
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Cationic liposome-mediated transfection of mammary epithelial cells with AAV-based plasmid DNA
… were used; calcium phosphate co-precipitation, DOTAP, DMRIE-C and LipofectAMINE. The highest transfection efficiency resulted from using LipofectAMINE, although this efficiency was relatively low, with only 2.1% of cells found to express β-galactosidase. To establish the efficacy of …
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Dual Delivery Systems Based On Polyamine Analog Benspm As Prodrug And Gene Delivery Vectors
… In addition, compared with LipoBEN and DOTAP, decreased transfection efficiency of Lipo-SS-BEN demonstrated instability of Lipo-SS-BEN in extracellular environment. </p> <p>In order to design a dual delivery vector with reduced vector toxicity and improved linker stability, we employed …
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Tie-ing the endothelium: development of an mRNA-based nanotherapeutic to restore microvascular barrier function for the treatment of sepsis-induced multiple organ failure
… SM-102 LNP formulation including cationic lipid DOTAP. The cell surface expression of Tie2 was upregulated in 13% of pulmonary endothelial cells, responsible for a 25% increase in overall Tie2 expression in lung tissue. In Chapter 3, a library of 36 ionizable APEs, synthesized via ring-opening …