Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 4 of 4 for “"DOP-PCR"”.
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Degenerate oligonucleotide primed amplification of genomic DNA for combinatorial screening libraries and strain enrichment
… using degenerate oligonucleotide primed PCR (DOP-PCR). The approach can produce DNA libraries from nanograms of a template genome or the metagenome of multiple microbial populations. The DOP-PCR primers contain random bases, and thermodynamics of hairpin formation was used to design …
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Genome complexity reduction for genome-wide single nucleotide polymorphism analysis
… SNP genotyping techniques all rely on an initial PCR amplification of each SNP locus. Individual or low-level multiplexed PCR reactions are sufficient for genotyping a few to a few hundred different SNPs, but genome-wide linkage and association studies in humans will require thousands to tens of …
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Degenerate Oligonucleotide Primed - Polymerase Chain Reaction Evaluation And Optimization To Improve Downstream Forensic STR Analysis Of Low Quality/Low Quantity DNA
… analyses. A commercially available kit for DOP-PCR, a form of WGA, is currently being used in the clinical for downstream single locus targets. Forensic analyses utilize a multiplex amplification. This study determined that the "home brew" created by our lab performs the same as the …
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New Methods of DNA Assembly, Gene Regulation with a Synthetic sRNA, and Cyanobacterium Phenotype Monitoring with Raman Spectroscopy
… of microbial cells. In this matter, λ-PCR (lambda-PCR) was developed enabling the construction of plasmid DNA. This technique allows DNA assembly and manipulation (insertion, substitution and/or deletion) at any location of a vector. λ-PCR addresses the need for an easy, …