Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 5 of 5 for “"DNS-Methyltransferase"”.
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Kupplung der DNA-Methyltransferase M.SssI mit Triplehelix-bildenden Oligodesoxynucleotiden
DNA modifying enzymes, like DNA methyltransferases (DNA MTases) and restriction endonucleases (REases), could in principle be used to map or manipulate genomes. However, these enzymes are inappropriate for that purpose because of their generally short recognition sequences. An increase of their …
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Biophysikalische Charakterisierung und biochemische Steuerung von Methyltransferase-DNA-Wechselwirkungen
… methods for the characterisation of DNA methyltransferase (MTase) interactions with DNA and their methodical direction by means of biochemical approaches. Taking the MTase M.TaqI as a paradigm, the kinetic analysis of these interactions is performed by stopped flow measurements with …
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Super-specific DNA methylation by a DNA methyltransferase coupled with a triple helix-forming oligonucleotide
… an engineered variant of the DNA cytosine-C5 methyltransferase (MTase) M.SssI has been conjugated with a triple helix-forming oligonucleotide (TFO) and the properties of the enzyme-TFO conjugate were evaluated using a variety of biochemical and biophysical methods. M.SssI is the only known …
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Funktionsbasierte Isolierung von Subproteomen mit trifunktionellen Fangverbindungen
… the first photoaktivable capture compound™ for methyltransferases was avaiable at Aachen University, it was still unclear how it should be implemented to obtain samples suitable to further mass spectrometric analysis. An important challenge during this work was therefore, working out suitable …
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Epigenetische Mechanismen zur Regulation der Transkription C 4-typischer Gene in Mais (Zea mays L.)
Nuclear genes are organised in chromatin. The transcriptional activation of chromatin is associated with various epigenetic mechanisms and covalent histone modifications play a central role in this process. Among these modifications, methylation and acetylation of the N-terminal regions of histones …