Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 41 for “"DNMT1"”.
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Investigation of hypothalamic molecular mechanisms underlying narcolepsy and obesity through DNMT1 and leptin receptor signalling
… first project explored whether mutations in the DNMT1 gene that cause autosomal dominant neurodegenerative disease in humans, which preferentially affects the hypocretin neurons that regulate sleep, also induce neuronal loss when introduced into mice. No evidence was found for the loss of …
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Regulation of DNA (Cytosine-5) Methyltransferase 1 in the cell cycle and its role(s) in doxorubicin-mediated micronuclei formation
The expression of DNMT1 is critical in coordinating DNMT1 activity with biological processes and therefore must be tightly regulated in the cell cycle. Several studies have shown that DNMT1 expression level is inversely correlated with p21WAF1 protein, but the exact mechanism remained unresolved. …
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DNA Methylation and DNA Methyltransferases in the Clonal Raider Ant, Ooceraea biroi
… DNA methylation primarily is carried out by DNMT1, which targets hemi – methylated DNA and maintains methylation patterns through cycles of cell replication, and DNMT3, the de novo methyltransferase. These genes are essential to mammalian development, and mutations lead to embryonic (DNMT1 …
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The roles of DNA(cytosine-5) methyltransferase1 in carcinogenesis related to cellular factors, virus and chemicals
… late genes, hence suppressing the early genes. DNMT1 and PCNA was found to interact with two distinct regions of Hsp70. The binding site of Hsp70 was localized to the N-terminal of DNMT1. The disruption of this site in DNMT1b abolished its interaction with Hsp70. Activation of the ATPase …
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Functional consequences of cytosine methylation in mitochondrial DNA catalyzed by DNA methyltransferase 1
… methyltransferase, DNA methyltransferase 1 (DNMT1) translocates to mitochondria, driven by an in-frame mitochondrial targeting sequence (MTS) located upstream of the nuclear DNMT1 translational start site. This MTS is highly conserved across mammalian species, and directs a heterologous …
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The Specific Role of the MLL CXXC Domain in MLL Fusion Protein Function
… CXXC domains from other proteins, including DNMT1, CGBP and MBD1, replaced the MLL CXXC domain in the context of an oncogenic MLL fusion. DNA binding experiments determined that the MLL CXXC domain has the highest DNA binding affinity, followed by the CGBP and DNMT1 CXXC domains. In vitro …
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An investigation of the epigenetic and transcriptional changes which follow Epstein-Barr virus infection of germinal centre B cells
… DNMT3A, and the down-regulation of DNMT1 and DNMT3B, a pattern of expression which is re-capitulated in HL cell lines. I have also shown that the major EBV oncogene, LMP1, is responsible for the down-regulation in GC B cells of DNMT1, and that DNMT3A binds to the EBV promoter, Wp …
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INVESTIGATING THE LINK BETWEEN HOMOLOGOUS RECOMBINATION PROTEIN BRCA2 AND DNA METHYLATION
… BRCA2 expression did not affect the function of DNMT1, the protein responsible for post replicative DNA methylation maintenance. We subsequently investigated whether DNMT1 influenced DNA replication and repair pathways. To this end, we employed an auxin-induced degron (AID) system for DNMT1 for …
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Synthetic approaches to the understanding of DNA nucleobase methylation
… (DNMTs). Among different mammalian DNMTs, DNMT1 is the most abundant and active, and plays multiple roles in carcinogenesis, embryonic development, and several other biological functions. Reactivation of silenced tumor suppressor genes by DNMT inhibitors (DNMTi) is provides a relatively new …
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DNA methylation: the "stable" epigenetic mark
… DNMT3A/3B and then faithfully maintained by DNMT1. Hence, intermediate methylation levels likely represent stochastic de novo establishment (DNMT3A/3B) and clonal maintenance (DNMT1) within the cell population. To test this, I subcloned single cells from both mouse embryonic fibroblasts …
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Epigenetic Modification of Phenotype in Intestinal Smooth Muscle Cells
… cells had increased DNA methyltransferase 1 (DNMT1) and histone deacetylase 2 (HDAC2) expression. ISMC from human strictures showed a decreased contractile phenotype, with increased levels of DNMT1 and HDAC5. Repeated passage human ISMC also had decreased expression of contractile markers SMA …
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The Regulation of Dna Methylation In Mammalian Development and Cancer
… methylation by impairing the recruitment of the Dnmt1-Uhrf1 complex to chromatin, thereby identifying a novel crosstalk between histone arginine methylation and DNA methylation. Moreover, I show that PRMT6 upregulation contributes to global DNA hypomethylation in cancer. Lastly, my work results …
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Epigenetic effects of benzo(a)pyrene in Fundulus heteroclitus and Danio rerio
… exposure did not change DNA methyltransferase 1 (DNMT1) mRNA expression at 48, 60 or 96 hours post fertilization (hpf) or nuclear DNMT1 enzyme activity at 96 hpf. Direct waterborne BaP treatment at 100 μg/L from 2.5 to 96 hpf to zebrafish embryos did significantly decrease global DNA methylation …
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Using synthetic biology to investigate human gene body DNA methylation
… of the major maintenance methyltransferase DNMT1 by GSK-3484862 treatment. To understand how gene body methylation functions at endogenous loci, I analysed data from HCT116 cells which had been treated with the DNMT1 inhibitor. I found that most genes were unaffected by loss of gene body …
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A COMPUTATIONAL STUDY ON BIOCHEMICAL REACTIONS OF SULFUR-CONTAINING COMPOUNDS
… Chapter 3 presents a novel 3D model of the human DNMT1-DNA complex from homology modelling and MD simulations. Chapter 4 presents a mechanistic study on the first two steps of the DNA methylation using the DFT method based on small model systems. Chapter 5 and Chapter 6 present a DFT study on H2S …
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Snail1-mediated Gene Repression Links EMT to Enhanced Biliary Epithelial Cell Survival and Lung Epithelial Cancer Cell Migration
… Our data showed that DNA-methyltransferase 1 (DNMT1) interacts with a long intergenic ncRNA (lincRNA-STXPB5) and Snail1 in A549 cells. DNMT1 activity was required to repress SCARA5 expression during TGF-β1-induced EMT in A549 cells. However, differing from a previous study suggesting that …
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An investigation of the contribution of cigarette smoking and human papillomavirus infection to the epigenetic modulation of cellular genes in cervical epithelium
… the up-regulation of the DNA methyltransferase DNMT1 which binds to the TSG, RARB, resulting in its de novo methylation. Specific CpG loci in the RARB promoter appeared to be targeted for de novo methylation, rather than methylation of all the CpGs, and may represent the methylation pattern seen …
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MicroRNA and Epigenetic Controls of CD4+ T cells' Activation, Differentiation and Maintenance
… TCR-PI3K activation leads to the accumulation of DNMT1, elevated CpG methylation in the foxp3 promoter, and suppression of foxp3 transcription. Furthermore, we demonstrated that an epigenetic regulator, methyl CpG binding protein 2 (MeCP2), is critical to sustain Foxp3 expression in Tregs, and to …
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The Regulation of UHRF1 during Mammalian Embryogenesis
… which recruits the maintenance methyltransferase DNMT1 to newly replicated DNA. This project set out to elucidate the mechanism(s) by which UHRF1 protein is regulated during epigenetic reprogramming. Further dissection of the regulation of DNA methylation machinery in naïve pluripotency highlights …
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