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Showing 1 to 20 of 30 for “"DNAzyme"”.
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Defining the substrate scope of DNAzyme catalysis for reductive amination with aliphatic amines
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2025-05-01
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The Treatment of Breast Cancer Tumor Growth and Metastasis With an Anti-MMP9 DNAzyme
… are of interest.</p> <p><strong>METHOD: </strong>DNAzymes are catalytic oligonucleotides that bind to and cleave specific mRNA, resulting in a decreased protein expression. The safety and efficacy of anti-MMP-9 DNAzyme (AM9D) <em>in vivo </em>was determined by injecting <sup>35</sup>S‑labeled AM9D …
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Applications of functional nucleic acids in imaging, sensing and drug delivery and investigations of DNAzyme-metal interactions
… including nucleic acids catalysts (ribozymes and DNAzymes) and ligands (aptamers), have been discovered in nature or isolated in a laboratory through a process called in vitro selection. They are nucleic acids with functions similar to protein enzymes or antibodies. They have been developed into …
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In vitro selection of monovalent metal ion-dependent DNAzymes
… reactions, these DNA sequences are called DNAzymes, or DNA enzymes. Like with protein enzymes, the sequence of a DNAzyme dictates the folding of DNA into unique tertiary structure which ultimately programs its catalytic function. The folding of DNA is a product of a combination of …
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Development of functional DNA-based sensors and investigations into their mechanism
… DNA molecules also known as deoxyribozymes or DNAzymes were first isolated in 1994 through an in vitro selection procedure and have since been engineered and isolated to perform various functions that include both RNA and DNA cleavage and ligation. The 8-17 DNAzyme is an RNA-cleaving DNAzyme …
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I. In vitro selection of aptamers for perchlorate and melamine II. Towards crystallization of DNAzymes III. A highly selective lead sensor based on a classic lead DNAzyme
… of functional nucleic acids, catalytic DNA (DNAzyme) has been isolated using a similar in vitro technique (in vitro selection) to catalyze the cleavage of a phosphodiester bond. Similar in principle, both SELEX and in vitro selection are combinatorial chemistry technique that enables the …
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Exploring the Structural and Functional Characteristics of a Catalytic DNA
Finally, the use of DNAzymes in biosensing applications was studied for Pb2+ detection. The molecular beacon DNAzyme biosensor was shown to be capable of detecting Pb2+ in soft to moderately hard water sources when used in conjunction with a handheld fluorometer. Detection limits under these …
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Immobilization of Catalytic DNA for Advanced Materials and Sensors
The DNAzyme was also immobilized on patterned glass surfaces via covalent attachment. Substrate-functionalized 100 nm polystyrene particles were then hybridized in the absence and presence of Pb2+. The result was the Pb2+-mediated tethering of particles onto patterned grids, where Pb2+ induced the …
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In vitro selection and characterization of mono-, di-, and trivalent metal-dependent DNAzymes and their sensing applications
… high affinity and selectivity. Catalytic DNA or DNAzymes, another class of functional nucleic acids, were first isolated from pools of random DNA in 1994 using the in vitro selection procedure to catalyze the cleavage of a phosphodiester bond. Since the discovery of the first aptamer and DNAzyme, …
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Functional dna: Biochemical/biophysical characterization & sensing applications
… together through the common thread of the 8-17 DNAzyme and involve biochemical and biophysical characterization of the 8-17 DNAzyme as well as characterization of a novel red Pb2+ species formed upon cleavage of a modified 8-17 DNAzyme. Determination of the enantiomeric ratio is important as …
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The Development of Gene Therapy by way of DNAzymes, and a Computational Tool that Predicts Efficient DNAzymes.
… target the androgen receptor (AR) is required. DNAzymes, single stranded antisense molecules constructed of DNA, have promise as a novel therapeutic strategy to down-regulate AR expression and therefore inhibit tumour growth. An assay pipeline of DNAzyme cleavage reactions, targeting the AR and …
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The in vitro selection and biochemical characterization of metalloDNAzymes
DNAzymes are strands of catalytic DNA. First discovered in 1994, they have proved themselves capable of catalyzing many different types of reactions with significant rate enhancements. Because they often require divalent metal-ion cofactors, DNAzymes have readily been developed into metal-ion …
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In Vitro Selection and Characterization of Transition Metal -Dependent DNAzymes and RNAzymes
"These DNAzymes are dependent on Co2+ for cleavage activity. By exploiting the electronic properties of Co2+, we have used UV-Visible spectroscopy to obtain a better understanding of the metal binding properties of the selected DNAzyme systems. Through analysis of the spectroscopic and metal …
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In vitro selection of DNAzymes selective for monovalent metal ions and their development as sensors in living cells and organisms
… the catalytic function (deoxyribozymes or DNAzymes) of DNAs have been explored since 1990s, which attract widespread interest. DNAzymes, fully biocompatible but not naturally identified, commonly use metal ions as cofactors for catalysis, and some of them show exceptional selectivity for …
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Fluorescent and Colorimetric Biosensors Based on DNAzymes and DNA Aptamers
… nucleic acids, a large number of catalytic DNA (DNAzymes) and aptamers have been isolated, many of which possess high analyte specificity. As a result, these functional nucleic acids can be used for sensing and diagnostic applications. However, the number of successful sensors made from these …
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In Vitro Selection and Metal Specificity of Transition Metal-Dependent DNAzymes
… of two families of Co2+-dependent DNAzymes further demonstrates the interplay between DNAzyme structure and metal specificity. The clone 11 and 18 DNAzymes were selected for study because the primary sequences differ by only four bases, producing vast differences in Co2+ …
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DNA Inspired Tools for Biosensor Design
… of a copper (II) (Cu (II)) specific DNA cleaving DNAzyme was undertaken towards the development of a sensitive Cu (II) sensor. Despite the promising utility of DNA cleaving DNAzymes, their slow kinetics limit their application in biosensors. Through studies in solution, it was discovered that the …
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Cellular metal ion sensing using DNAzymes
… metal ions or to meet new desired criteria. DNAzymes, DNA sequences with catalytic activity, have recently emerged as an alternative class of sensors for metal ions. As selection of DNAzymes for different metal ions is carried out through a combinatorial process, new sequences with desired …
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Fluorescence resonance energy transfer study of the global folding of functional DNAs and electrohydrodynamic printing of protein arrays
… conformational change of the UO22+ specific DNAzyme 39E. Inactive metal ions, Mg2+ and Zn2+ are found capable of inducing folding between two pairs of stems while the active ions UO22+ and Pb2+ are not able to bring about the folding in any pairs of the stems. By correlating the enzymatic …
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G-Triple Chemiluminescent DNAZyme Based Biosensor for the Detection of Calcium(II) Ions in Solution and the Biological Functionality of Kinase Enzymes and the Rationale for their Employment as Promising Drug Targets, Substrate Specific Inhibition of P38alpha
… as recognition elements in bioanalytical assays. DNAzymes are functionally active, synthetic single-stranded DNA molecules that are capable of catalyzing biological reactions similarly to proteins or RNAzymes. The majority of DNAzymes are synthesized by an in vitro selection method known as …
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