Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 21 for “"DNA-helicase"”.
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Homologous Strand Exchange and DNA Helicase Activities in Plant Mitochondria
… organisms by exchange and rearrangement of DNA. Most of our knowledge about homologous recombination is limited to processes in bacteria or in eukaryotic nuclei. In E. coli, homologous recombination is dependent on the RecA protein. Higher plant chloroplasts have RecA-like strand exchange …
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Single-molecule study of the DNA helicase regulating genomic stability
A DNA helicase, PcrA is an essential protein in gram-positive bacteria. PcrA utilizes its helicase activity and translocation activity to regulate various cellular functions including plasmid replication and counteracting deleterious recombination. Using single molecule FRET technique and …
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Treslin and its role in the assembly of the replicative DNA helicase
DNA replication is an essential biochemical process that underlies genetic inheritance. In eukaryotic cells, this process is carried out by a multi-subunit protein complex called replisome. The core of replisome is made up of six-subunit protein ring Mcm2–7, which contains weak DNA helicase …
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Mechanism of Mcm10 function during DNA replication
… do so with limited errors. In eukaryotic cells, DNA replication is accomplished by a multi-stage process involving numerous protein assemblies. The core component of this process is the replicative DNA helicase, Mcm2-7. Mcm2-7 complexes are loaded around origins of DNA replication during G1 …
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Molecular Characterization of Mth203 protein
… systems to understand the conserved events in DNA replication and other processes. Methanothermobacter thermautotrophicus, a thermophilic archaeon has a circular genome and a single origin of replication. It’s replication initiation proteins MthCdc6-1 and MthCdc6-2 are homologues of the …
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Molecular characterization of Mth203 protein
… systems to understand the conserved events in DNA replication and other processes. Methanothermobacter thermautotrophicus, a thermophilic archaeon has a circular genome and a single origin of replication. It’s replication initiation proteins MthCdc6-1 and MthCdc6-2 are homologues of the …
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Modeling and Simulating Biomolecular Machineries at Atomic Scale and Beyond
… which are simple and ubiquitous, namely, (1) a DNA helicase PcrA, a prototype molecular motor traveling along DNA, for which we have provided evidence for an alternating domain mobilities mechanism that controls the directional translocation; (2) a membrane channel protein Aquaporin-1 (AQP1), …
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Single-molecule studies of the mechanism of eukaryotic helicase activation
Eukaryotic DNA replication is a fundamental process that must occur accurately and only once per cell cycle. To ensure that origins only initiate once per cell cycle, the events of DNA replication initiation are temporally separated to different phases of the cell cycle. This regulation separates …
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Multiple roles of the replication initiation protein Cdtl during helicase loading in S. cerevisiae
… and activation of the eukaryotic replicative DNA helicase, the hetero-hexameric Mcm2-7 complex. As cells enter G1 phase of the cell cycle, all potential sites of replication initiation are selected by the loading of inactive Mcm2-7 double hexamers. The anti-parallel orientation of the Mcm2-7 …
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Increased Oxidative Phosphorylation In An Ipsc- Derived Model of Rothmund-Thomson Syndrome Associated Osteosarcomagenesis
… with Type II RTS have biallelic mutations in the DNA helicase RECQL4, which has been shown to have mitochondrial functions that include DNA replication and repair. We describe the generation of 12 induced pluripotent stem cell lines (iPSCs) from two paired RTS patient/ parental control (Family) …
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Mobile genetic elements in Coxiella burnetii: Friends, foes or just indifferent?
… interrupt the 23S rRNA gene; an intein within dnaB (encoding replicative DNA helicase) and a homing endonuclease. The introns are self-splicing ribozymes and able to inhibit ribosome function and retard bacterial growth through internal guide sequence (IGS)-dependent and -independent …
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Mobile genetic elements in Coxiella burnetii: Friends, foes or just indifferent?
… interrupt the 23S rRNA gene; an intein within dnaB (encoding replicative DNA helicase) and a homing endonuclease. The introns are self-splicing ribozymes and able to inhibit ribosome function and retard bacterial growth through internal guide sequence (IGS)-dependent and -independent …
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Biogenesis and Redox Transition of Iron-sulfur Clusters in Proteins
… clusters. The first example is Rtel1, a DNA helicase that regulates telomere length. Rtel1 was predicted to contain an iron-sulfur cluster, but this was not demonstrated. The third part of this work shows that the N-terminal domain of Rtel1 indeed contains a redox active [4Fe-4S] cluster. …
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Transcription Coupled DNA Repair in Saccharomyces Cerevisiae: the Interplay of Facilitators and Repressors
… that removes bulky and/or helix-distorting DNA lesions, such as UV induced cyclobutane pyrimidine dimers (CPDs) and bulky chemical adducts. Transcription coupled repair (TCR) is a subpathway of NER dedicated to rapid removal of lesions in the transcribed strand of actively transcribed genes. …
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The Molecular Mechanism of Break Induced Replication
DNA double strand break (DSB) is one of the most threatening of all types of DNA damages as it leads to a complete breakage of the chromosome. The cell has evolved several mechanisms to repair DSBs, one of which is break-induced replication (BIR). BIR repair of DSBs occurs through invasion of one …
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In vitro reconstitution of the ubiquitylation and disassembly of the eukaryotic replisome
… is copied only once. The Cdc45-MCM-GINS (CMG) DNA helicase which is the core of the eukaryotic replication complex, has been shown to be extensively regulated by post translational modifications, during its assembly. Therefore, it is not inconceivable that the process to unload the replication …
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Single molecule force spectroscopy of single stranded DNA binding protein and Rep helicase
Single-stranded DNA binding (SSB) proteins are essential accessory proteins that protect single-stranded DNA (ssDNA) during genome maintenance. Escherichia coli SSB is a prototypical homo-tetrameric SSB protein that can wrap up to 65 nucleotides of ssDNA in one of its binding modes. Here we present …
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Theoretical and computational investigations into structure-function relationships of biomolecular machines at atomic resolution
… roles in many cellular processes, including DNA replication and repair, gene expression, protein degradation, protein secretion and maintenance of pH homeostasis. Despite intense research efforts, the atomic-level mechanism transmitting other form of energy into mechanical force is still …
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Functional Characterisation of a RECQL4 Mutation in Rothmund-Thomson Syndrome
Germline mutations affecting the RECQL4 DNA helicase cause Type II Rothmund-Thomson syndrome (RTS), a human disease characterised by defects in skeletal development and predisposition to specific types of cancer, including osteosarcoma (OS). RECQL4 has been implicated in multiple cellular functions …
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Conjugative transfer and expression of an integrative and conjugative element in Pectobacterium atrosepticum : A thesis submitted in partial fulfilment of the requirements for the Degree of Doctor of Philosophy at Lincoln University
… genes related to cell partitioning systems and a DNA helicase for effective transfer by conjugation. To understand whether HAI2 was likely to be transferred to related bacteria within the ecological niche of Pectobacterium atrosepticum SCRI1043, conjugation assays were conducted in vitro using …
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