Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 3 of 3 for “"DNA-cutting"”.
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Discovering and Characterizing New Homing Endonucleases for Genome Engineering
… (LHEs) are a family of highly specific DNA-cutting enzymes capable of recognizing target sequences of ~20 bp. In many eukaryotes, including humans and yeast, double-strand breaks induced by LHEs stimulate repair by Homologous Recombination, which can be used to alter or repair a gene if …
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Enabling the in vitro study of long noncoding RNAs to understand their role in Plasmodium falciparum
… enzymes were explored including commonly used DNA-cutting enzymes (Cas9), inactivated enzymes to block transcription (dCpf1) and enzymes that target RNA directly (Cas13), the latter of which had not been applied to *Plasmodium*. Furthermore, I implemented these tools to demonstrate the …
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A study of protein-DNA interactions using atomic force microscopy and DNA origami
… environments, where the tolerance for imprecise cutting is relatively high, in vivo treatment remains difficult. Most genome editing tools are derived from naturally occurring regulatory proteins. Better understanding of the mechanisms used by these natural proteins should facilitate the use of …