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Showing 1 to 20 of 96 for “"DNA-binding protein"”.
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The DNA Binding Protein Lsr2 from Mycobacterium tuberculosis
Lsr2 is a small, basic DNA binding protein that is highly conserved in mycobacteria and related actinomycetes. Lsr2 is essential for growth in Mycobacterium tuberculosis and previous studies have shown that Lsr2 is involved in down-regulating a wide range of genes involved in cell wall synthesis …
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Cloning and characterization of a single-stranded DNA-binding protein of Leishmania major
… referred to as leishmaniasis. The major surface protein on all species of Leishmania is a highly abundant 63 kDa glycoprotein referred to as GP63. GP63 has been characterized as a cell surface protease, however, its exact role in the Leishmania life cycle is not clear. The genes encoding GP63 are …
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Single molecule force spectroscopy of single stranded DNA binding protein and Rep helicase
Single-stranded DNA binding (SSB) proteins are essential accessory proteins that protect single-stranded DNA (ssDNA) during genome maintenance. Escherichia coli SSB is a prototypical homo-tetrameric SSB protein that can wrap up to 65 nucleotides of ssDNA in one of its binding modes. Here we present …
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The effect of single-stranded DNA binding protein RPA2 on XPD helicase processivity
Understanding how proteins work together to perform vital cellular functions, such as replicating and repairing DNA, not only extends our understanding of fundamental biology, but can also lead to important medical interventions. As a necessary first step to understanding larger systems, we focus …
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Purification and Characterization of a Methyl-DNA Binding Protein Complex from Primary Erythroid Cells
… expression is mediated at least in part by DNA methylation.A methyl-DNA binding complex, termed the MeCPC (Erythroid Methyl Cytosine-binding Protein Complex), has been found to bind to the methylated, but not unmethylated, ρ-globin promoter and proximal transcribed region in nuclear extracts …
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Characterization of the Sigma Factor Proteins and the Dna Binding Protein Euo of Chlamydia
… a cascade of sigma factor expression and the binding of the early stage protein EUO to DNA. </p> <p> The protein expression levels of each of the three sigma factors were monitored throughout the developmental cycle to determine if expression of the sigma factors is consistent with a role in …
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Binding configurations of single-stranded DNA binding protein and their influence on DNA recombinase
DNA inside a cell is continuously damaged through multiple mechanisms including environmental exposure to radiation, chemical agents, or UV light. Certain products of the cell's own metabolism, such as reactive oxygen species, can also damage the DNA. In the worst-case scenario, this damage results …
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Structural Studies of a Non-Sequence-Specific DNA Binding Protein, Hmg-D, Complexed With DNA
An HMG box is a DNA binding domain common to a large number of proteins whose diversity in function range from transcriptional activation and repression to chromatin assembly. These proteins can be viewed as two classes divided by their ability to bind DNA in a sequence-specific vs. non-specific …
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Cloning a Putative DNA-Binding Protein Controlling the 5'LTR of the Copia Element in Drosophila
… expression, are located within the 5'LTR. The DNA sequence in the 5'LTR and in the location between downstream of entire the 5' LTR and the initial translation site have been identified by mobilityshift binding assays and DNase I footprinting assays. The data reveals three protected regions: a …
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The PHD finger protein 5 is a part of the spliceosome and acts as a DNA-binding protein
Vier Proteine: U2AF35, SRp40, mDomino und Ddx1 wurden durch Screening Yeast-two hybride-Bibliothek als Partner des PHF 5a-Protein gefunden. Proteininteraktionen wurden in-vitro und in-vivo bestätigt. Die für Bindungen verantwortlichen Domäne wurden als RS Domäne (reich an Arginin-Serin) …
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1. X-ray crystallographic analyses of the gene V protein from Ff bacteriophage, a single-stranded DNA-binding protein, and its Y41 mutants 2. X-ray crystallographic analyses of several anticancer drugs and their interactions with DNA
In biological systems, DNA/RNA-protein interactions as well as drug-DNA interactions play important roles in genetic processes. A fundamental understanding of these processes requires a detailed structural knowledge of DNAs, proteins, drugs, as well as their complexes with each other. Gene V …
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DNA -Binding Proteins That Facilitate Integration of Transmissible DNA Elements in Bacteroides Thetaiotaomicron
… utilizing heparin column, size-exclusion and DNA-affinity chromatography, 2-D gel electrophoresis and mass fingerprinting to identify proteins that bound the attachment sites of CTnDOT (attDOT ) and NBU1 (attNI), and stimulated integration in vitro for both DNA elements. The proteins isolated …
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TDP-43 Is Directed to Stress Granules by Sorbitol, a Novel Physiological Osmotic and Oxidative Stressor
TDP-43, or TAR DNA-binding protein 43, is a pathological marker of a spectrum of neurodegenerative disorders including amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration with ubiquitin-positive inclusions (FTLD-U). TDP-43 is an RNA/DNA-binding protein implicated in …
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Purification of the proteins that bind to the gp-2 promoter in Dictyostelium discoideum
… sites. I present here the purification of a DNA binding protein that binds to the 3' "C" box sequence in the <i>gp</i>-2 promoter using a DEAE Sephacel resin and specific "C" DNA affinity column chromatography. With undifferentiated amoebae cell extract, a DNA binding protein migrated at 0.40 …
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Roles for Chd7 in Zebrafish Development with Implications for Human Disease
<p>The Chromodomain Helicase DNA Binding Protein (CHD) family consists of a group of nine known proteins that function in controlling DNA dynamics and transcription. The CHD family member of interest in this work, <em>Chromodomain helicase DNA-binding protein 7 (chd7)</em>, has been implicated in …
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Exploring the Mitigation of TDP-43 Toxicity by Sis1 in Yeast
… there is no cure. Research has revealed that Tar DNA-binding Protein 43 cytoplasmic aggregates are involved in many cases of Amyotrophic Lateral Sclerosis. One possible mechanism for TDP-43 induced toxicity is that these aggregates are titrating away a molecular chaperone protein, Sis1, from the …
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Biochemical and genetic analysis of nucleic acid-protein interactions
… Escherichia coli is a small, sequence-specific DNA-binding protein. The specific and nonspecific DNA binding constants were determined by gel mobility shift assays. The binding constant of IHF for the H$\sp\prime$ site in $\lambda$ attP is 6.8 $\times$ 10$\sp8$ M$\sp{-1}$ and the nonspecific …
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Experimental studies of RctB- initiator of Vibrio cholerae chromosome II replication: genetic engineering, protein purification, and protein crystals
… maintained. In V. cholerae, the initiation of DNA replication in each chromosome involves distinct processes. While the initiation of replication of chromosome I is carried by DnaA, the initiation of chromosome II replication is controlled by RctB, a DNA-binding protein that is an exclusive …
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X-ray diffraction analysis of gene V protein encoded by filamentous bacteriophage Ff
The gene V protein encoded by bacteriophage Ff is a single-stranded DNA binding protein and it plays an important role in the replication cycle of the phage. The crystal structure of the gene V protein was determined using multiwavelength anomalous diffraction on the selenomethionine-containing …
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