Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 64 for “"DNA-binding activity"”.
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The Impact of Selected Gene Mutations to the Activation and DNA Binding Activity of the Transcription Factor VraR
… by phosphorylation resulted in a weaker binding to DNA. Moreover, we investigated VraR E59D, S164P, A113V, and S164A binding activity to the PvraSR by DNase-I footprinting. Finally, in vivo studies on the effects of substitutions of the above residues on the mRNA level of a number of …
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Mcm10 regulates the turnover of DNA polymerase alpha.
… Critical for Mcm10's role at the fork is its DNA binding activity. Utilizing the crystal structure of the conserved internal domain of X. laevis (x) Mcm10, we identified residues that, when mutated, significantly reduced xMcm10's ability to bind DNA in vitro. Importantly, the corresponding …
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An Investigation for Genetic Susceptibility to Type 1 Diabetes Mellitus and its Microvascular Complications
… factor, heat shock factor-1 (HSF-1) and NFκB-DNA binding activity in response to a concentration of 31 mM D- glucose in peripheral blood mononuclear cells (PBMCs) from patients with TIDM with and without microvascular complications. Hyperglycaemia induced significant increases in both NFκB and …
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Ehrlichia chaffeensis Type 1 Secreted Effector TRP47 is a Novel SUMOylated Nucleomodulin
… SUMOylation and as a novel nucleomodulin with DNA-binding activity and host nuclear localization. Using a microfluidic chip peptide array, a SUMOylation site in the TRP47 N-terminal domain was identified. A SUMO-2 modification was further confirmed using an in vitro SUMOylation assay with …
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Functional stability of a nucleic acid-hydrolyzing single chain antibody in various biochemical and biophysical environments
… fragment (scFv) antibody has cell-penetrating activity, nucleic acid-binding activity, nucleic acid-hydrolyzing activity, and antiviral activity. As a first step toward development of antiviral reagent using 3D8 scFv, functional stability based on DNA-binding and -hydrolyzing activity of 3D8 …
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An assessment of the effects of novel anti-inflammatory compounds in cell based studies
… pathway induced NF-κB and AP-1 transcriptional activity respectively. However, all tested compounds inhibited NF-κB transcriptional activity; in particular AIK18/85/1 prevented the TNF-α-induced translocation of NF-κB (p65) to the nucleus assessed by indirect immunoflouresnce. This effect of …
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Role of ETV5 Mutations in B Cell Acute Lymphoblastic Leukemia
… ETV5 mutations alter its ability to bind target DNA sequences, resulting in altered gene expression that promotes leukemia. Electrophoretic mobility shift assays demonstrated that R392P and V444I impaired the ability of ETV5 to bind to its cognate binding site (5’-CCGGAA-3’). In contrast, T505A …
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Transcriptional regulation in Plasmodium falciparum: characterisation of TBP-like protein
… is hitherto poorly understood. The TATA-binding protein (TBP) is a universal transcription factor required for transcription by RNA polymerase I, II and III. Multiple gene duplication events throughout eukaryotic evolution have resulted in many eukaryotes harbouring more than one TBP …
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Fatty acid regulation of adhesion molecule expression in human vascular cells.
… calcified lesion. Adhesion molecules control the binding of the leukocytes to the endothelium and their subsequent extravasation in to the arterial intima. They are expressed on the activated endothelium and on leukocytes. Intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion …
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Microvascular endothelial response to cocaethylene exposure: morphological and molecular observations
… phosphorylation, and transcription factor DNA binding activity (Aims Three and Four). Aim One experiments demonstrated a lack of overt endothelial cytotoxicity caused by CE. Aim Two morphological analysis of endothelial intercellular borders and barrier integrity showed that CE exposure in …
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Expression and initial characterisation of the Plasmodium falciparum general transcription factors TFIIB and TLP
… is known about both the key protein factors and DNA elements which guide the assembly of RNAPII components into the transcription initiation complex. Bioinformatics studies have shown that there is very little amino acid sequence conservation between human and Plasmodium RNAPII transcription …
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TGA2 dual activity: N-terminus regulation of reversible DNA binding influenced by NPRI and CK2
… The N-terminus has also been shown to affect DNA binding of the TGA2 bZIP domain when phosphorylated by Casein Kinase II (CK2). The mechanisms involved for directing a switch from basal repressor to activator, and the role of kinase activity, have not previously been looked at in detail. This …
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HIF, HAPE, and Hilltop rats: a paradox unfolding
… it was hypothesized that differences in HIF-1α activity or HIF-1 induced proteins would explain, in part, the apparent paradox. Methods: Hilltop and Madison rats were subjected to zero, 6 or 18 hours of acute hypobaria (18,000 ft). Pulmonary edema was documented by blood free ww/dw (BFww/dw) …
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Effect of disruption of 3D8 complementarity-determining regions on properties of 3D8 antibody
… antibodies have been utilized to improve antigen-binding affinity and/or stability. However, little is reported about functional consequence by the disruption of whole amino acid sequence in CDRs of an antibody with multiple activities. Here, we dissected influence of each whole CDR-disruption on …
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Potential oxygen-dependent regulation of PIAS3
… of the JAK/STAT pathway, binds to and blocks the DNA-binding activity of signal transducer and activator of transcription 3 (STAT3). PIAS3 has the potential to be hydroxylated at a specific proline residue, in an amino acid sequence known to be involved in oxygen-dependent post-translational …
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The role of steroidogenic factor-1 (SF-1) in gonadotropin-releasing hormone (GnRH) receptor gene regulation
… the presence of a factor with SF-1-like DNA-binding activity in the gonadotrope cell lines, αT3-1 and αT4, by gel retardation assays. DNasel footprinting reveals that the major DNA-binding activity in αT3-1 cells on the GnRHR promoter occurs at the SF-1-like site. The SF-1-like sequence …
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Functional Characterization of Plasmodium falciparum TATA-box binding-like Protein (PfTLP)
… (Bing, 2014; Milton, 2017), including a TATA box-binding-like protein, PfTLP. Bioinformatic analysis suggested that PfTLP is a TRF2-type TBP-like protein. However, PfTLP differs in several aspects from previously characterized TRF2-type proteins. These differences are thought to be Plasmodium …
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Mechanical Manipulation of Eukaryotic Chromatin by DNA-Binding Proteins
… serves to exert spatiotemporal control of many DNA-transacting processes such as gene expression. Despite emerging progress, the biophysical mechanism underpinning eukaryotic genome organization remains an outstanding question in the field. In this thesis, I describe mechanistic insights on …
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Evidence that ARNT plays a role in the regulation of the immunoglobulin heavy chain enhancer and identification of a putative ARNT ligand
… our investigation we discovered a putative ARNT -binding ligand that increases DNA-binding activity of the ARNT homodimer. This ligand was partially characterized by UV crosslinking studies and a variety of biochemical studies using electrophoretic mobility-shift assays. Preliminary data suggests …
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Control of uteroglobin gene expression
… factors that influence RNA polymerase II activity. Included among these factors are the cellular receptors for steroid hormones. The rabbit uteroglobin gene expression is positively stimulated by progesterone, and binding sites for the progesterone receptor have been identified in distal …
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