Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 27 for “"DNA-Protein Interactions"”.
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DNA-Protein Interactions in f1 DNA Replication
<p>This thesis analyzes the interaction of two DNA-binding proteins with the plus strand replication origin of bacteriophage f1. The origin has a bipartite structure consisting of a required core origin region and an adjacent A+T- rich enhancer sequence that potentiates replication approximately …
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Visualization, Kinetics, and Thermodynamics of DNA-Protein Interactions
… thermodynamic and structural information about DNA–protein interactions. Furthermore, the micrographs obtained by cryo-TEM were compared to AFM images taken in a previous work. The main goal of this work was to investigate the influence of surfaces on DNA–protein interactions, therefore the …
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DNA -Protein Interactions in the CTnDOT Excisive Intasome
… to date. Excision requires multiple accessory proteins. Exc is one protein required for excision, however, its function is unknown. In an attempt to determine a role for Exc, the native wild type Exc protein was purified. These studies indicated that Exc might interact with IntDOT during …
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DNA-protein interactions throughout the Arabidopsis calmodulin-3 gene
… nuclear factors, termed high mobility group proteins, which are thought to play an architectural role in regulating the chromatin structure of a gene. Sequence specific DNA-binding proteins that recognize sequence elements in the 5$\prime$-flanking, intron, and 3$\prime$ untranslated region …
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Convex lens induced confinement : a novel study of weak and slow DNA & protein interactions
… previously unobtainable probing of slow and weak DNA and protein interactions. Such measurements are made possible by CLIC's simultaneously improved signal-to-background ratio, observation time, observation volume, and permissible molecule concentration. The 10, 000 increase in the observation …
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The development of a model system and high throughput assay for the study of interactions between zinc finger proteins and DNA
… a full code of amino acid-based recognition of DNA sequences would be useful. Several approaches, which utilise small DNA binding motifs called zinc fingers, are presently employed. None of the current approaches successfully combine a combinatorial approach to the elucidation of a code with a …
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Studies of sequence recognition by the EcoRI restriction/modification system of Escherichia coli
… nucleotide sequence-specific enzymes and binding proteins. Models have proposed a variety of means for site location, including formation of hydrogen bond matrices between protein and DNA and ionic contacts between the ribose-phosphate backbone and basic amino acids residues on the protein. We …
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Novel Applications of Biolayer Interferometry to Investigate Molecular Interactions
… study, BLI is used to explore various molecular interactions to demonstrate molecular binding and to provide quantitative characterization of binding kinetics and affinities of phage-protein and DNA-protein interactions. The results of this study demonstrate novel use of biolayer interferometry …
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Applications of fluorescence detected sedimentation I Studying the thermodynamic impact of valence in non-ideal solutions II High affinity interactions and biological media
… fluorophore allowing the study of high affinity interactions (Kd ∼ pM) not previously accessible with the absorbance optics. The types of high affinity interactions that can be explored include drug-protein interactions, lipid-receptor interactions, DNA-protein interactions and …
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The role of Bacteroides Host Factor A in CTnDOT integration and Holliday junction resolution
… in an in vitro integration assay. I purified DNA binding proteins from a Bacteroides thetaiotaomicron strain lacking known ICEs. The purified fractions were then tested in the in vitro integration assay. The active fraction contained a protein that we named BHFa for Bacteroides Host Factor A. …
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The genomic anchors of the human major histocompatibility complex
… the anchoring of chromatin to the insoluble proteinaceous fraction of the nucleus known as the nuclear matrix. Since only a few MARs have been characterised so far, their role in genomic structure and function is not well understood. The aim of this thesis is to use the human Major …
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The mechanism of cyanamide/MMS-induced DDI2/3 expression in Saccharomyces cerevisiae
… DDI2 and DDI3, can be highly induced by DNA-damaging agent methyl methanesulfonate (MMS). They share identical coding sequence and their promoter sequences are also highly conserved, which are referred as DDI2/3 in this dissertation. Subsequent studies revealed that DDI2/3 encode a …
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1, Structural and Functional Studies of Human Replication Protein A; 2 DNA Damage Responses and DNA Repair Defects in Laminopathy-Based Premature Aging.
… of mammalian cells is under constant attack from DNA-damaging agents. To maintain genomic integrity, cells activate an array of pathways primarily consisting of DNA repair and DNA damage checkpoints. Human replication protein A (RPA), a single-stranded DNA (ssDNA) binding protein, is essential for …
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Using Synthetic Nanopores for Single -Molecule Analyses: Detecting SNPs, Trapping DNA Molecules, and the Prospects for Sequencing DNA
This work focuses on studying properties of DNA molecules and DNA-protein interactions using synthetic nanopores, and it examines the prospects of sequencing DNA using synthetic nanopores. We have developed a method for discriminating between alleles that uses a synthetic nanopore to measure the …
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Identification of Regulatory Binding Sites and Corresponding Transcription Factors Involved in the Developmental Control of 5'-nucleotidase Expression in Dictyostelium discoideum
… shift assays were performed to identify specific DNA-protein interactions on the 5nt promoter. These assays enabled the detection of a 0.13 Rf and 0.33 Rf binding activity to specific sites of the promoter. After characterization of these binding activities, both proteins were purified by a series …
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Molecular Mechanisms Underlying Aerobic Respiration and Heme Regulation in Yeast
… hypoxia tolerance on a genome-wide scale, protein localization was studied using green fluorescence protein (GFP) tagged library of yeast genes under normoxia and hypoxia by Henke and colleagues. This study identified more than 200 proteins that change their localization under hypoxia. …
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Bacterial expression of radio-labeled recombinant proteins for studying AHR signalling
… factor Aryl Hydrocarbon Receptor (AHR) forms a DNA binding heterodimer with the Aryl Hydrocarbon Nuclear Translocator (ARNT) in response to planar aromatic hydrocarbons. In addition to AHR and ARNT there are at least three other proteins involved in AHR signaling. These proteins are the …
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The Mapping of Transcription Factor Binding Sites in the Turkey Prolactin Gene
… participate in tissue- and sequence-specific DNA-protein interactions with nuclear extracts from turkey pituitaries. These are the regions from nucleotides (nt) -41 to -73, -105 to -137, and -175 to -199, named tprl-1, tprl-2 and tprl-3, respectively. Three shifted bands were observed using …
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The Regulation of Alkaline Phosphatase during the Development of Dictyostelium
… post translational modification, and the rate of protein degradation. The developmental control of <i>alkaline phosphatase</i> (alp) in <i>Dicytostelium</i> has provided a focal point for the study of gene regulation at the level of <i>de novo</i> synthesis. The localization of <i>alkaline …
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Microfluidic Technology for Low-Input Epigenomic Analysis
Epigenetic modifications, such as DNA methylation and histone modifications, play important roles in gene expression and regulation, and are highly involved in cellular processes such as stem cell pluripotency/differentiation and tumorigenesis. Chromatin immunoprecipitation (ChIP) is the technique …
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