Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"DNA-PKcs"”.
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Integration of ATM, ATR, and DNA-PKcs Signaling Maintains Genome Integrity During Neurogenesis
<p>The DNA damage response (DDR) orchestrates a network of cellular processes such as cell cycle progression, DNA repair, and apoptosis when complex DNA lesions arise to maintain genomic integrity. ATM, ATR, and DNA-PKcs (encoded by PRKDC) are related phosphatidylinositol-3-kinase like …
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Sensing Foreign DNA: The Role of DNA-PKcs in Human Anti-viral Innate Immunity
… patterns (PAMPs), such as viral genomic DNA, which is a major PAMP during DNA virus infection. Viral DNA sensing leads to the activation of the STING-TBK1-IRF3 signalling axis and the production of type I interferon. Previously, our work identified the non-homologous end-joining protein …
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DNA-PKcs/ Artemis Complex in DNA Double-Strand-Break Repair: Cryo-EM and Biochemical Studies
DNA is the main carrier of inheritance and DNA damage will thus have serious consequences. DNA double-strand breaks (DSB) are amongst the most lethal forms of DNA damage. One DSB can lead to catastrophic consequences including cancer and cell death. To fix it, there are two main mechanisms-- …
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An exploration of the interplay between HSV-1 and the non-homologous end joining proteins PAXX and DNA-PKcs
DNA damage response (DDR) pathways are essential in maintaining genomic integrity in cells, but many DDR proteins have other important functions such as in the innate immune sensing of cytoplasmic DNA. Some DDR proteins are known to be beneficial or restrictive to viral infection, but most remain …
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Isolation and Functional Mapping of Human T-Cell Leukemia Virus Type 1 Tax Oncoprotein DNA-Damage Complexes
… through interaction with components of the DNA damage response pathway, promoting cellular genomic instability. We examined cellular Tax complexes in an effort to elucidate potential protein-protein interactions that can model the Tax-induced molecular events.</p> <p>We also investigated the …
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Of mice, genes, and radiation: the genetics of non-hereditary breast cancer explored using the common laboratory BALB/c mouse
… mice carrying alternate genetic variants of the DNA repair gene Prkdc (DNA-PKcs). Strains congenic for the common (PrkdcB6) and variant (Prkdc BALB) alleles of Prkdc are developed, genotypically validated, and used to examine the functional consequences of Prkdc BALB and its linkage with …
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Development of Novel Technologies to Investigate DNA Double-Strand Break Repair Uncovers a Role for the ATM Kinase in Error-Free NHEJ with Implications for Neurodegenerative Diseases
DNA double strand breaks (DSBs) are considered to be the most lethal genotoxic lesion because they can result in chromosomal translocations or result in a major loss of genetic information if repaired incorrectly. To preserve genomic integrity, mammalian cells have evolved a set of complimentary …
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Targeting the DNA damage response in hormone-driven cancers
… (BC) and prostate cancer (PCa). Defects in the DNA damage response (DDR) as well as replication stress are responsible, in part, for driving genomic instability and accumulation of DNA damage. While the resulting alterations can be beneficial for tumour development and progression, it also …
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Cellular pathology and apoptosis in experimental and human acute and chronic compressive myelopathy.
… at 24 hours and 1 week post-injury. PARP, DNA-PKcs and AIF immunopositivity occurred in glia at, above and below the site. APP immunopositivity was present in axonal swellings. In human chronic compression, axonal swellings, loss of anterior horn cells, and cystic change were seen in severe …
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DNA repair and sister chromatid exchange
… of SCE within telomeric regions. The role of DNA repair in SCE regulation and formation is also under investigation. While it has been shown that at least one of the DNA repair pathways, homologous recombination (HR), is likely to be involved in the formation of SCE, it is less clear whether …
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Mechanisms of Immunogenic Cell Death in Tumour Cells Infected with Oncolytic Vaccinia Virus
… we focused on the interaction between host DNA-repair capabilities and oVACV infection. We tested whether mutations in the DNA repair proteins DNA-PKcs, BRCA1 or BRCA2 had an impact on oVACV-induced death and found that BCRA2-deficient cells were more sensitive to oVACV killing than their …
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Targeting DNA Double-Strand Break Repair to Potentiate Radio- and Chemo Therapy of Glioblastoma
… was due to the augmented repair of TMZ-induced DNA double strand breaks (DSBs). TMZ induces DNA replication-associated DSBs that are repaired primarily by the homologous recombination (HR) pathway. We found that cell lines from recurrent cultures exhibited faster resolution of Rad51 foci and …
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Investigation of The Roles of Asf1 and Caf-1-Mediated Chromatin Assembly In The Human Dna Damage Response
… model for the role of chromatin in DNA repair infers that chromatin is a mere obstacle to DNA repair. However, here we show that blocking chromatin assembly of newly-synthesized histones, via knockdown of the histone chaperones ASF1A, CAF-1 or a mutation that specifically prevents …
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Artemis Interacts With The Cul4A Ubiquitin E3 Ligase Complex and Regulates The Cell Cycle Progression
… by the phosphatidylinositol 3-kinases (PIKs) DNA-PKcs, ATM and ATR in response to a variety of cellular stresses. Artemis has important roles in V(D)J recombination, DNA double strand breaks repair and damage-induced cell-cycle checkpoint regulation. The detailed mechanism by which Artemis …
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The Role of RUVBL1/RUVBL2 and Their Potential as Therapeutic Targets in Non-Small Cell Lung Cancer
… suggested that RUVBL1/2 may have roles in DNA replication in NSCLC, and inhibition or depletion of RUVBL1/2 in sensitive NSCLC lines delays S-phase progression and ultimately results in cancer cell death via replication catastrophe. While Compound B treatment in vivo produces modest …
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Single-molecule studies reveal mechanisms of human DNA double-strand break repair
DNA damage is ubiquitous to all organisms and very complex pathways have evolved to recognize and repair these lesions. The most deleterious DNA damages are double-strand breaks (DSBs), and a single unrepaired DSB can lead to cell death. In human cells, there exist two canonical pathways of DSB …
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Wirkung schwerer Ionen auf strahlenresistente und strahlensensitive Tumorzellen
… untersucht. Zusätzlich wurden auch die an der DNA-PK-abhängigen NHEJ-Reparatur beteiligten Proteine (DNA-PKCS, Ku 70 und Ku 86) untersucht. Als Modellsystem wurden zwei etablierte Tumorzelllinien (WiDr und MCF-7) ausgewählt, die eine unterschiedliche intrinsische Strahlensensitivität gegenüber …
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Examination of molecular genetic factors involved in sensitivity to breast cancer following radiation exposure
Understanding DNA repair is not only an important aspect of cell biology, but also has important implications for the field of carcinogenesis since cancer most likely occurs from genetic damage that occurs over one's lifetime. DNA repair needs to be accurate and efficient in order for a cell to …
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Determining molecular mechanisms of DNA Non-Homologous End Joining proteins
DNA double strand breaks (DSB), particularly those induced by ionizing radiation (IR) are complex lesions and if not repaired, these breaks can lead to genomic instability, chromosomal abnormalities and cell death. IR-induced DSB often have DNA termini modifications including thymine glycols, ring …
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