Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 25 for “"DNA-PK"”.
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Determining molecular mechanisms of DNA Non-Homologous End Joining proteins
DNA double strand breaks (DSB), particularly those induced by ionizing radiation (IR) are complex lesions and if not repaired, these breaks can lead to genomic instability, chromosomal abnormalities and cell death. IR-induced DSB often have DNA termini modifications including thymine glycols, ring …
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Study of the DNA Damage Complexes Within the HTLV-1 Tax Oncoprotein Interactome
… instability via repression of the cellular DNA damage repair response. Our laboratory has previously shown that the interaction between Tax and various proteins involved in the DNA-damage response pathway impairs the ability of these proteins to mount an efficient repair response. As part of …
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Novel Roles of Replication Protein A Phosphorylation in Cellular Response to DNA Damage
… protein A (RPA) is an eukaryotic single-stranded DNA binding protein directly involved in a variety of DNA metabolic pathways including replication, recombination, DNA damage checkpoints and signaling, as well as all DNA repair pathways. This project presents 2 novel roles of RPA in the cellular …
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The role of linker histone variant in double strand break repair
Eukaryotic DNA is packaged into chromatin, providing a physical and structural barrier for regulatory and effector proteins to access the DNA. It has been shown that linker histone variants inhibit the double strand break (DSB) repair pathway known as non-homologous end joining (NHEJ). NHEJ, in …
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Isolation and Functional Mapping of Human T-Cell Leukemia Virus Type 1 Tax Oncoprotein DNA-Damage Complexes
… through interaction with components of the DNA damage response pathway, promoting cellular genomic instability. We examined cellular Tax complexes in an effort to elucidate potential protein-protein interactions that can model the Tax-induced molecular events.</p> <p>We also investigated the …
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Role of the Innate Immune Response to DNA During Therapy with Oncolytic Viruses
… killing virus (SKV), through cytoplasmic DNA sensing pathways and how manipulation of this pathway may impact OV efficacy. Here, I screened several syngeneic murine cancer cell lines for their expression of cytoplasmic DNA sensing proteins and susceptibility to OV infection. After …
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Nuclear Basic Fibroblast Growth Factor Regulation of Triple-Negative Breast Cancer Dormancy/Recurrence
… we identify a novel signaling axis [nuclear bFGF/DNA-dependent protein kinase (DNA-PK)] supported by chemotherapy-enriched dormant TN breast cancer cells. This signaling axis drives accelerated DNA repair in chemo-residual TN breast cancer cells. Targeting this axis with either with a bFGF shRNA …
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Widespread Phosphorylation of H2AX by Adenovirus Requires Viral Genome Replication and the E1B-55k or E4orf3 Proteins
Adenovirus infection activates cellular DNA damage response and repair pathways. The viral E1B-55K and E4orf3 proteins prevent recognition of viral genomes as a substrate for DNA repair by targeting members of the sensor complex composed of Mre11/Rad50/NBS1 for degradation and relocalization, as …
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Investigations into the vaccinia virus immunomodulatory proteins C4 and C16
… has two reported functions. Firstly, it inhibits DNA-PK-mediated DNA sensing, and this study found that C4 can perform this function as well. Like C16, C4 associates with the Ku heterodimer to block its binding to DNA leading to reduced production of cytokines and chemokines. For both proteins, …
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The circadian Control of DNA end Resection
… for the circadian clock in the regulation of DNA end resection, the licensing step of HDR. Thus, we demonstrated that the circadian factor CRY1 drives circadian oscillations in DNA end resection, which results in circadian fluctuations in HDR. Mechanistically, we show how this protein is …
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Inhibition of PLK1 by p53 and by PLK1-targeted drugs
… arrest in prometaphase and activation of the DNA damage response. Part of this thesis aimed at investigating the DNA damage response (DDR) induced in mitosis by PLK1 inhibitor and the consequences of such inhibition. Also, the results were compared with the results obtained/reported in …
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Identification And Functional Analyses Of Novel Protein Interactions And Post-Translational Modifications For The Transcription Factor Deformed Epidermal Autoregulatory Factor-1.
… of DEAF-1. A region encompassing the DNA binding domain of DEAF-1 interacts with the C-terminal Bax interacting domain of the Ku70 subunit of the DNA-PK holoenzyme. Ku70 acts as an anti-apoptotic protein through C-terminal domain and so DEAF-1 was assessed for its ability to influence …
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DNA damage responses in the context of the cell division cycle
During my PhD, I have investigated aspects of the DNA damage response (DDR) in the context of three different cellular scenarios: DNA damage signalling in response to double-strand breaks during mitosis, coordination of DNA replication with DNA damage responses by regulation of the GINS complex, …
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Integration of ATM, ATR, and DNA-PKcs Signaling Maintains Genome Integrity During Neurogenesis
<p>The DNA damage response (DDR) orchestrates a network of cellular processes such as cell cycle progression, DNA repair, and apoptosis when complex DNA lesions arise to maintain genomic integrity. ATM, ATR, and DNA-PKcs (encoded by PRKDC) are related phosphatidylinositol-3-kinase like …
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Identifying a role for Ciz1 in the DNA damage response
In proliferating cells, cell cycle arrest and DNA repair are induced upon detection of DNA damage. The DNA damage response (DDR) to double strand DNA breaks is mediated by phosphatidylinositol 3-kinase-like protein kinases (PIKKs), which propagate DDR signalling by phosphorylating SQ/TQ motifs in …
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Double-Strand Break Repair Mechanisms in Human Embryonic Stem Cells
… cell culture. The most detrimental form of DNA damage is the DNA double-strand break (DSB), which is managed by cells through complex mechanisms, designated the DNA damage response. There are two major types of DSB repair; homologous recombination repair (HRR) and non-homologous end joining …
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Dissecting the Role of microRNAs in the Radiation Response of Human Prostate Cancer
… transition (EMT) and promoting DNA repair following radiation, we found that miR-875-5p reconstitution in PCa cells counteracted EMT and impaired DNA lesion clearance, mainly via impairment of EGFR-ZEB1 axis. Restoration of miR-205 was able to significantly enhance PCa …
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Single-molecule studies reveal mechanisms of human DNA double-strand break repair
DNA damage is ubiquitous to all organisms and very complex pathways have evolved to recognize and repair these lesions. The most deleterious DNA damages are double-strand breaks (DSBs), and a single unrepaired DSB can lead to cell death. In human cells, there exist two canonical pathways of DSB …
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Wirkung schwerer Ionen auf strahlenresistente und strahlensensitive Tumorzellen
… untersucht. Zusätzlich wurden auch die an der DNA-PK-abhängigen NHEJ-Reparatur beteiligten Proteine (DNA-PKCS, Ku 70 und Ku 86) untersucht. Als Modellsystem wurden zwei etablierte Tumorzelllinien (WiDr und MCF-7) ausgewählt, die eine unterschiedliche intrinsische Strahlensensitivität gegenüber …
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Sensing Foreign DNA: The Role of DNA-PKcs in Human Anti-viral Innate Immunity
… patterns (PAMPs), such as viral genomic DNA, which is a major PAMP during DNA virus infection. Viral DNA sensing leads to the activation of the STING-TBK1-IRF3 signalling axis and the production of type I interferon. Previously, our work identified the non-homologous end-joining protein …
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