Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 6 of 6 for “"DNA-PAINT"”.
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Quantitative DNA-paint imaging of AMPA receptors in live neurons
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2023-12-01
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Quantitative super-resolution imaging of cell polarity proteins using DNA-PAINT
… densely-packed assemblies is still challenging. DNA-based point accumulation for imaging in nanoscale topography (DNA-PAINT) is one of the most recently developed imaging techniques in super-resolution microscopy. It uses fluorescently-labelled DNA to visualise the molecules of interest with …
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Fluorescence fluctuation spectroscopy and microscopy : application to cellular molecular dynamics and organization
… for Imaging in Nanoscale Topography (PAINT), that enables highly multiplexed super-resolution imaging of synaptic proteins. We employed DNA-PAINT to resolve nano-scale organization of seven targets simultaneously including synaptic proteins and cytoskeletal markers. These approaches …
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CHEMICAL BIOLOGY STRATEGIES FOR THE FORMATION AND CLEAVAGE OF CHEMICAL BONDS
… preparation of site-specific and plasma stable DNA-protein conjugates which are suitable for cellular applications. Using the method developed, various types of ONs can be easily conjugated to a range of proteins via solvent accessible cysteine residues, including therapeutic full-length IgG …
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Single-Objective Tilted Light Sheet Illumination with Exchange-PAINT and Deep Learning for Fast, Accurate, and Precise 3D Single-Molecule Super-Resolution Imaging in Mammalian Cells
… light sheet into the sample, (iii) sequential DNA Point Accumulation for Imaging in Nanoscale Topography (DNA-PAINT) known as Exchange-PAINT, (iv) engineered point spread functions (PSFs) for 3D imaging, and (v) deep learning for high-density localization of single molecules. Altogether, this …
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Development of single-molecules techniques to study native α-Synuclein aggregates
… SiMPull with a super-resolution technique, DNA-PAINT, which enabled αSyn structures to be viewed and characterised with nanometer resolution (∼ 20 nm). Overall, the work presented here describes methodologies developed to detect and characterise native αSyn oligomers with a high degree of …