Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"DNA vaccines"”.
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Novel intranasal GNRs-DNA vaccines against human papillomavirus infection
Currently licensed HPV vaccines, Cervarix and Gardasil, have been successfulin preventing HPV infection and high-graded cervical intraepithelial neoplasia (CIN2,3) in school girls in the UK and other developed countries. However, their high costshave hindered their wider use in preventing HPV …
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Single chain MHC trimer-based DNA vaccines for pathogen protection
… has been an important strategy for successful vaccines. However, most immunization approaches with class I binding peptides have failed to induce CD8+ T cell responses strong enough to prevent disease. This failure has been attributed to the lack of CD4+ T cell help and difficulty in …
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Development of plasmid dna formulations for application as microspheric dna vaccines
The advent of DNA vaccines has heralded a new technology allowing the design and elicitation of immune responses more adequate for a wider range of pathogens. The formulation of these vaccines into the desired dosage forms extends their capability in terms of stability, routes of administration and …
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Formulation and characterisation of cationic microparticles for the delivery of DNA vaccines
… microparticle vector for the delivery of DNA vaccines. The work builds upon previous research by Singh et al which described the adsorption of DNA to the surface of poly (D,L-lactide-co-glycolide) (PLG) microparticles stabilised with the surfactant cetyltrimethyl ammonium bromide (CT AB). …
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Immunological evaluation of DNA vaccines against Haemonchus contortus and Toxoplasma gondii in sheep
Plasmid DNA vaccines which encode selected antigenic proteins direct protein expression in mammalian cells after vaccination. The use of DNA vaccines has been shown to be a promising approach to induce specific humoral and cellular immune responses against various pathogens, such as parasites. In …
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Investigations into DNA vaccination against Channel Catfish Virus
… Virus (CCV). Studies conducted to evaluate DNA vaccination against CCV compared encoded gene, dose, multiple DNA vaccines, and immune response to vaccination. Genes were selected (ORFs 1 and 3 [immediate early genes], ORFs 6, 19, and 46 [membrane genes], and ORF59 [putative major envelope …
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Modification of a DNA Vaccine for Oral Administration in Fish for Aquaculture by Using Non-Microbial Nanoparticles
Utilization of DNA vaccines in aquaculture has been gaining interest and recent efforts have been focused on methods of delivering DNA vaccines to fish. In the present study, a methodology was sought that could protect DNA vaccines such that they could be orally administered. The main objective of …
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Evaluation of polyelectrolyte multilayer thin-film coated microneedle arrays for transcutaneous vaccine delivery
… organ for the safe and convenient delivery of vaccines, small molecules, and other biologics. Members of the Irvine and Hammond groups have developed a polyelectrolyte multilayer thin film-coated microneedle platform that can achieve simultaneous DNA and nanoparticle delivery. This delivery …
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The design, development and characterization of a self-replicating DNA expression technology
… protective immunity. Among current methods, DNA vaccines, generate highly specific T-cell immunity towards targeted antigens, and do not suffer from issues like misdirected vector targeted immunity, like viral based vectors. DNA vaccines, however, face a variety of their own weaknesses. These …
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Role of dendritic cells in response to cytokine gene adjuvants
DNA vaccination is a novel means of expressing antigens in vivo for the generation of humoral and cell-mediated immune responses. DNA vaccines elicit protective immunity in mice; however, DNA vaccination in outbred species requires optimisation. Dendritic cells (DC) are unique in that they are the …
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Towards the development of plant-made PsVs as potential delivery vehicles for therapeutic HPV vaccines
… with >70% of all cervical cancers. While current vaccines can prevent infection with high-risk HPVs, they cannot induce regression of persistent infections, thus the development of vaccines that function therapeutically is required. DNA vaccines are ideal candidates for therapeutic treatment; …
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Investigation of the immunostimulatory activity and vaccine potential of lipid encapsulated plasmid DNA and oligodeoxynucleoties
DNA vaccines offer unique promise as a means of generating immunity against infectious and malignant disease. Unfortunately a number of obstacles, including rapid degradation of naked plasmid DNA (pDNA), poor cellular uptake by antigen presenting cells (APCs) and subsequent low levels of gene …
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Microscale controlled continuous cell culture
… of commercially relevant chemicals such as DNA vaccines are comparable to large scale bench fermentations. Continuous cultures are also demonstrated without contamination for 3 weeks in a single device and both steady state and dynamically controlled conditions are possible, allowing …
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Evaluation of a Tetravalent DNA Vaccine against Dengue: Integrating Biochemical Studies on Dengue Virus Envelope Protein to a Domain-Based Antigen Design.
… Concerns regarding the effectiveness of Dengue vaccines together with emergence of Zika virus (ZIKV) have reignited the interest for new vaccines using alternative approaches. Given that the envelope glycoprotein (E) is the main target of neutralizing antibodies, it has been used as the antigen …
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Attenuated Salmonella Typhimurium Can Be Used to Transduce Human Dendritic Cells Towards Vaccination Against HIV-1
<p>The promise of DNA vaccination is limited in part by invasive, untargeted administration. We refined the strategy by incorporating attenuated bacteria to deliver plasmids directly to APCs. Initial studies involved human macrophages, which were infected with aroASalmonella typhimurium transformed …
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Incorporation of tumor antigen epitopes into cytokine signal peptides enhance the efficacy of immuno-gene therapy
… cytokine expression plasmids as DNA vaccines. Our data showed that immunization with an OVA epitope-modified IL-2 expression plasmid resulted in protective immune responses to an OVA expressing tumor. In summary, the work presented here demonstrated that enhanced immunological …
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Development of new tools for the production of plasmid DNA biopharmaceuticals
DNA vaccines and gene therapies that use plasmid DNA (pDNA) as a vector have gained attention in recent years for their good safety profile, ease of manufacturing, and potential to treat a host of diseases. With this interest comes increased demand for high-yield manufacturing processes. Overall, …
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Immunologic and Protective Effects of Vaccines for Mycobacterium marinum in Morone sp
Recombinant and DNA vaccines utilizing Mycobacterium sp. antigen 85A (Ag85A) were assessed for immunostimulatory and protective effects against M. marinum. Because of their known susceptibility to piscine mycobacteriosis, Morone sp. were utilized as the models for these studies. The first study …
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Working towards a plant-produced Bovine papillomavirus vaccine: expression of BPV1 virus-like particles and pseudovirions in Nicotiana benthamiana
Bovine papillomaviruses (BPVs) are DNA viruses implicated in several diseases and cancers of considerable veterinary and agricultural importance in cattle, horses, as well as several wild animal species, leading to their economic depreciation through the deterioration of their function and …
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