Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 28 for “"DNA vaccine"”.
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The Efficacy of DNA Vaccine Using a Sindbis Virus-based Vector Against Nucleocapsid Protein of Hantaan Virus
… 2% and 10%. But, there is no effective and safe vaccine against HFRS. Even though neutralizing antibodies against the HTNV have been proven to be critical against viral infections, the cellular immune responses to HTNV are also assumed to be important for viral clearance. This study has examined …
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Modification of a DNA Vaccine for Oral Administration in Fish for Aquaculture by Using Non-Microbial Nanoparticles
Utilization of DNA vaccines in aquaculture has been gaining interest and recent efforts have been focused on methods of delivering DNA vaccines to fish. In the present study, a methodology was sought that could protect DNA vaccines such that they could be orally administered. The main objective of …
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Effects of low pathogenicity avian influenza on turkey breeding flocks and immune evaluation of an H1N2 DNA vaccine
… post-vaccination using traditional IAV killed vaccines has been challenging. In the present study, a plasmid-based DNA vaccine expressing the HA gene of an H1N2 LPAI virus in addition with immunostimulatory genes was developed and evaluated in turkeys. Turkeys were challenged with an H1N2 virus …
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Evaluation of a Tetravalent DNA Vaccine against Dengue: Integrating Biochemical Studies on Dengue Virus Envelope Protein to a Domain-Based Antigen Design.
… Concerns regarding the effectiveness of Dengue vaccines together with emergence of Zika virus (ZIKV) have reignited the interest for new vaccines using alternative approaches. Given that the envelope glycoprotein (E) is the main target of neutralizing antibodies, it has been used as the antigen …
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Immunogenic assessment of plant-produced Human papillomavirus type 16 chimaeric L1:L2 virus-like particles and the production of an encapsidated therapeutic DNA vaccine candidate
… an estimated 266 000 deaths every year. Current vaccines are based on the immunodominant L1 major capsid protein, which assembles into virus-like particles (VLPs) that are highly effective in type-specific prevention of cervical infection. However, these vaccines are produced in expensive cell …
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Construction and characterization of chimaeric human immunodefiency virus type 1 subtype C Gag virus-like particles
… (VLPs) as a boost to the HIV-IC multigene DNA vaccine pTHr.grttnC, which encodes a modified Gag-RT-Tat-Nef fusion protein (GRTTN). Furthermore, an attempt was made to produce VLP analogues to the HIV-IA polyepitope DNA vaccine pTHr.HIVA. A range of in-frame fusions with the C-termini of …
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Immunologic and Protective Effects of Vaccines for Mycobacterium marinum in Morone sp
Recombinant and DNA vaccines utilizing Mycobacterium sp. antigen 85A (Ag85A) were assessed for immunostimulatory and protective effects against M. marinum. Because of their known susceptibility to piscine mycobacteriosis, Morone sp. were utilized as the models for these studies. The first study …
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Investigation of the immunostimulatory activity and vaccine potential of lipid encapsulated plasmid DNA and oligodeoxynucleoties
DNA vaccines offer unique promise as a means of generating immunity against infectious and malignant disease. Unfortunately a number of obstacles, including rapid degradation of naked plasmid DNA (pDNA), poor cellular uptake by antigen presenting cells (APCs) and subsequent low levels of gene …
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Single chain MHC trimer-based DNA vaccines for pathogen protection
… has been an important strategy for successful vaccines. However, most immunization approaches with class I binding peptides have failed to induce CD8+ T cell responses strong enough to prevent disease. This failure has been attributed to the lack of CD4+ T cell help and difficulty in …
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Development of plasmid dna formulations for application as microspheric dna vaccines
The advent of DNA vaccines has heralded a new technology allowing the design and elicitation of immune responses more adequate for a wider range of pathogens. The formulation of these vaccines into the desired dosage forms extends their capability in terms of stability, routes of administration and …
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Investigations into DNA vaccination against Channel Catfish Virus
… Virus (CCV). Studies conducted to evaluate DNA vaccination against CCV compared encoded gene, dose, multiple DNA vaccines, and immune response to vaccination. Genes were selected (ORFs 1 and 3 [immediate early genes], ORFs 6, 19, and 46 [membrane genes], and ORF59 [putative major envelope …
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Development of new tools for the production of plasmid DNA biopharmaceuticals
DNA vaccines and gene therapies that use plasmid DNA (pDNA) as a vector have gained attention in recent years for their good safety profile, ease of manufacturing, and potential to treat a host of diseases. With this interest comes increased demand for high-yield manufacturing processes. Overall, …
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Beak and feather disease virus candidate vaccine development
… psittacine species. There is currently no vaccine against BFDV and safe and affordable vaccine candidates are needed to alleviate the disease burden caused by this virus. Production of the BFDV's major antigenic determinant, the capsid protein (CP), in the inexpensive and highly scalable …
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Towards the development of plant-made PsVs as potential delivery vehicles for therapeutic HPV vaccines
… with >70% of all cervical cancers. While current vaccines can prevent infection with high-risk HPVs, they cannot induce regression of persistent infections, thus the development of vaccines that function therapeutically is required. DNA vaccines are ideal candidates for therapeutic treatment; …
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Construction and evaluation of three candidate vaccines expressing HIV-1 subtype-C mosaic Gag
… makes it challenging to develop effective vaccines that target a wide range of HIV subtypes. Mosaic immunogens have been computationally designed to specifically overcome this hurdle by maximizing the inclusion of common T cell epitopes. When compared to consensus immunogens, polyvalent …
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Characterisation of high and low avidity peptide specific CD8+ T cells using immunologic, transcriptomic and proteomic tools
… the peptide(s) accountable for the generation of vaccine specific responses are unknown such as whole protein or cell vaccines. Therefore, there is a need to identify markers which can quickly and reliably identify a high avidity T cell response in cancer vaccination settings. To achieve this …
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The Development and Assessment of Immune Responses in Plasmid DNA, VSV-Vectored, and Virus-Like Particle HIV Vaccinations in Murine Models
… Vesicular Stomatitis Virus (VSV)-vectored HIV vaccine (VSVXN2∆G/CDO-EBOVGP+HIV-1 Codon-optimized A74_Env-EC/SIV_Env-TMCT) in Rhesus macaques provided protection to 50% of the animals against low-dose challenges with SHIVenv_SF162P infection, making it an effective vaccine candidate. Building on …
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Vaccine Development Strategies for Crimean-Congo Hemorrhagic Fever Virus and its Tick Vector
… signs of disease from infection with CCHFV, vaccine development has focused on preventing human disease. Despite extensive research, no vaccine candidates have advanced to clinical trials. This dissertation aimed to utilize different vaccine platform technologies to target unique points in …
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Formulation and characterisation of cationic microparticles for the delivery of DNA vaccines
… microparticle vector for the delivery of DNA vaccines. The work builds upon previous research by Singh et al which described the adsorption of DNA to the surface of poly (D,L-lactide-co-glycolide) (PLG) microparticles stabilised with the surfactant cetyltrimethyl ammonium bromide (CT AB). …
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