Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"DNA vaccination"”.
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Investigations into DNA vaccination against Channel Catfish Virus
… Virus (CCV). Studies conducted to evaluate DNA vaccination against CCV compared encoded gene, dose, multiple DNA vaccines, and immune response to vaccination. Genes were selected (ORFs 1 and 3 [immediate early genes], ORFs 6, 19, and 46 [membrane genes], and ORF59 [putative major envelope …
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Engineering Secreted Proteins for Gene Transfer and DNA Vaccination
… the treatment of autoimmune disease using gene vaccination, human GAD65 was engineered to be secreted by mammalian cells. GAD65 is a major autoimmune antigen involved in the development of type I diabetes in both non-obese diabetic (NOD) mice and human patients. After the removal of the …
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Vaccination with DNA encoding a myelin autoantigen exacerbates experimental autoimmune encephalitis
… is to express the target autoantigen by DNA vaccination. In this work, the potential of vaccination with a DNA construct encoding the myelin oligodendrocyte glycoprotein (MOG), an important candidate autoantigen in multiple sclerosis, to induce tolerance and protect against experimental …
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The Efficacy of DNA Vaccine Using a Sindbis Virus-based Vector Against Nucleocapsid Protein of Hantaan Virus
… protein (NP) elicited by virus infection or DNA vaccination to investigate the immunogenicity of NP. To examine the cellular immune response against HTNV NP, C57BL/6 mice were injected with HTNV intraperitoneal. The NP-specific CD8+ T cell response was analyzed using a 51Cr-release assay, …
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The effect of nfa1 accination against Naeglearia fowleri infection
… gene was constructed and characterized for nfa1 DNA vaccination in mice. The expression of nfa1 gene in Chinese hamster ovary cell and human primary nasal epithelial cell transfected with the pQCXIN/egfp-nfa1 vector or pCDH/egfp-nfa1 vector was observed by fluorescent microscopy and Western …
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Nanostructured gene and drug delivery systems based on molecular self-assembly
… linear-dendritic "hybrid" polymers with plasmid DNA for applications in gene therapy. It begins with an overview of the design of next-generation, non-viral gene delivery systems and continues through the synthesis and validation of hybrid polymer systems, which possess modular functionalities …
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Role of dendritic cells in response to cytokine gene adjuvants
DNA vaccination is a novel means of expressing antigens in vivo for the generation of humoral and cell-mediated immune responses. DNA vaccines elicit protective immunity in mice; however, DNA vaccination in outbred species requires optimisation. Dendritic cells (DC) are unique in that they are the …
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Formulation and characterisation of cationic microparticles for the delivery of DNA vaccines
… microparticle vector for the delivery of DNA vaccines. The work builds upon previous research by Singh et al which described the adsorption of DNA to the surface of poly (D,L-lactide-co-glycolide) (PLG) microparticles stabilised with the surfactant cetyltrimethyl ammonium bromide (CT AB). …
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Role of Staphylococcus aureus GapC and GapB in immunity and pathogenesis of bovine mastitis
… S. aureus intramammary infections. Most mastitis vaccination strategies have focused on the enhancement of systemic humoral immunity rather than strengthening local intramammary immunity. Vaccines aimed at enhancing intramammary immunity of dairy cows against S. aureus mastitis have had limited …
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Attenuated Salmonella Typhimurium Can Be Used to Transduce Human Dendritic Cells Towards Vaccination Against HIV-1
<p>The promise of DNA vaccination is limited in part by invasive, untargeted administration. We refined the strategy by incorporating attenuated bacteria to deliver plasmids directly to APCs. Initial studies involved human macrophages, which were infected with aroASalmonella typhimurium transformed …
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Strategies for the induction of mucosal immunity against hepatitis B virus.
… systemic but rarely mucosal immunity. Novel vaccination strategies capable of inducing both systemic and mucosal immune responses could greatly reduce infection and morbidity. One such strategy is DNA vaccination whereby the antigen is synthesized in vivo after direct introduction of its …
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Single chain MHC trimer-based DNA vaccines for pathogen protection
… potential as a platform for antigen-specific DNA vaccines. Although there have been several reports of SCT-based DNA vaccines generating antigen-specific CTL responses, there have been no reports of pathogen protection after DNA vaccine expression of SCTs. In this thesis, I examined the …
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Generation and characterization of HIV-1 subtype C candidate vaccines that will induce high titre antibody responses to HIV-1 envelope glycoprotein
… linker, and to introduce an I559P mutation. DNA and modified vaccinia virus Ankara (MVA) vaccines were generated where Env was truncated to gp150, thereby retaining the transmembrane domain and a partial cytoplasmic tail (Env). The Env sequence for the protein vaccine was further trimmed by …
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Aspects of the Immunobiology of Myelin Oligodendrocyte Glycoprotein (MOG)-induced Experimental Autoimmune Encephalomyelitis (EAE)
… As demonstrated in this study, DNA vaccination using a plasmid encoding a myelin antigen is one approach to generate high titre autoantibody responses directed against the native protein. The pathogenicity of this antibody response can then be assayed in the same animal by …