Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"DNA uptake"”.
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Investigating the role of apoptosis regulator EndoG on exogenous DNA uptake, stability, replication and recombination
… the cell. It non-specifically cleaves endogenous DNA following apoptosis induction, but is also active in non-apoptotic cells for mitochondrial DNA (mtDNA) replication and may also be important for replication, repair and recombination of genomic DNA. The aim of our study was to examine whether …
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Molecular dissection of competence regulated virulence in Streptococcus pneumoniae
… the competent state naturally and acquiring DNA from the environment for recombination. In addition to improving genome plasticity, a natural competence system directly contributes to pneumococcal virulence during pneumonia and bacteremia, independent of DNA uptake. Competence is activated …
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Molecular Genetics in Rhodopseudomonas Sphaeroides: Transformation, Plasmids, and Gene Cloning
… pH 7.2, at 0(DEGREES)C prior to exposure to DNA was found to be necessary for inducing a competent state for DNA uptake. Polyethylene glycol (PEG) and Ca('2+) were also determined to be necessary components of this system and were added at the time of DNA addition to Tris-treated cells. The …
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Characterization of Type IV Pilus System Genes and Their Regulation in Clostridium perfringens
… aggregation, biofilm formation, adherence, and DNA uptake. Because few examples of T4P-utilizing Gram-positive bacteria are studied to date, we wanted to characterize the T4P system in this Gr+ bacterium. To understand the regulation of T4P genes and therefore better understand their expression, …
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Approaches Towards the Synthesis of Bradyoxetin
… in Staphylococcus aureus, competence for DNA-uptake in Bacillus subtilis and Streptococcus pneumoniae, sporulation in Bacillus subtilis, conjugal plasmid transfer in Enterococcus faecalis, and bacteriocin production in lactic acid bacteria. The collapse of (AHL) signaling system in …
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Lateral Transfer of DNA in Protocell-like Synthetic Cells
… electroporation can mediate the transfer of DNA into and between synthetic cells, potentially enabling gene expression in initially DNA-free compartments. Both TXTL and PURE-based cell-free expression systems were encapsulated in lipid in order to evaluate fluorescence as a proxy for GFP …
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A Fast Assay of Bacteria Cell Permeability for Genetic Transformation
… One method of intracellular delivery of foreign DNA during the genetic engineering process is the use of electroporation to create pores along the bacterial cell membrane. Current methods for assessing pore formation do not directly measure cell permeabilization or enable same-day assessment. In …
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Haemophilus influenzae as a chassis for directed endosymbiosis
… genetically tractable, naturally competent for DNA uptake, facultatively intracellular, and dependent on host-relevant cofactors including NAD and hemin. These features make it attractive for engineering bacterial endosymbionts. First, H. influenzae strains lacking the restriction endonuclease …
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Enhanced polymeric nanoparticles for gene delivery
… of poly([beta]-amino esters) (PBAEs) and DNA can be formulated to be stable in the presence of serum proteins and have high gene delivery without toxicity to human primary cells. The biophysical properties of PBAE/DNA nanoparticles have good correlation to transfection efficacy when tested …
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Tales of Seduction and Betrayal: Host Genes Required for Assault of Salmonella by Bacteriophage P22
… life cycle, especially at the step where phage DNA is translocated across the cytoplasmic membrane into the cell. Bacteria with mutations in any of these host factors required by the phage would gain resistance to infection by P22. Consequently, chemically mutagenized bacteria were exposed to a …
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Laboratory evolution and natural transformation in Acinetobacter baylyi ADP1
… requirement for the efficient transformation of DNA into the ADP1 genome, and incomplete knowledge regarding the composition and regulation of the protein complex involved in DNA uptake. Chapters 1 and 2 describe an investigation of the evolutionary stability of competence. We conducted a 1000 …
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Functional Characterization of the Streptococcus sanguinis com Regulon
… competence, the ability to acquire exogenous DNA into its genome. In the well characterized system of S. pneumoniae, genetic competence requires a quorum sensing system encoded by the early comCDE genes, as well as late genes controlled by the alternate sigma factor, ComX. Previous studies in …
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Dynamics of Cell Fate Decision Making Between Sporulation and Competence in Bacillus Subtilis
… under stress can either become competent for DNA uptake or undergo sporulation. The master regulator of sporulation is the transcription factor Spo0A. Single cell measurements of Spo0A dynamics along with activities of stage-specific sporulation reporters Spo0F, SpoIIE and SpoIIR revealed the …
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Expanding the Genetic Toolkit of Fusobacterium nucleatum by Generation of Fully-Sequenced Genomes and Discovery of Natural Competence
… notably, FusoPortal facilitated my discovery of DNA uptake machinery involved in natural competence and transformation in F. nucleatum. This work is the first to characterize natural competence in a Fusobacterium species, and also enables the expansion of Fusobacterium genetics utilizing the …
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Synthesis and evaluation of novel non-viral gene delivery systems for prostate cancer targeting
… the improvement in the transfection and cellular uptake of the modified dendriplexes in PC-3, DU145 and LNCaP prostate cancer cell lines. Finally, in vivo studies were carried out using BALB/c nude mice to investigate the anticancer effects of the Lf-bearing dendriplexes encoding TNFα, TRAIL, or …
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Characterisation of the ATP-dependent ligase, Lig E: An investigation into its significance in Neisseria gonorrhoeae
… rates due to the bacterium’s ability to take up DNA from its environment without regulation. With approximately 106 million new cases annually, thorough investigations into novel gonococcal pathways that can be targeted to limit its survival and spread are crucial. One possible target is the …
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Towards Novel Methods of Mutagenesis for Histophilus somni
… and may be enhanced by the presence of uptake signal sequences (USS) within the genome. We hypothesized that natural transformation occurs in H. somni because its genome is over-represented with USS and contains all the necessary genes for competence, except that ComD and ComE are …
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The contribution of competence system to the virulence of Streptococcus pneumoniae
… regulon. One important feature of pneumococcal DNA uptake during competence is the degradation of one strand of donor DNA by the pneumococcal membrane nuclease EndA. EndA-mediated DNA degradation and small DNA fragment release were used as indicators of competence development. Interestingly, …
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Endocytosis of therapeutic macromolecules in tumor cells - Mechanistic aspects of the proteoglycan receptor function
… sulfate proteoglycans (HS PGs) for efficient uptake, however the details of this mechanism and the exact role of the PG has been unclear. The aim of this thesis was to clarify the role of the cell surface PG in macromolecular uptake processes. It is demonstrated that mammalian cells can …
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Komparative Genomanalyse zur Stammoptimierung produktionsnaher Bacillus-Stämme
B. licheniformis DSM13 ist ein gram+ , ubiquitär vorkommendes, sporenbildendes Bakterium, das in großem Maßstab für die industrielle Produktion von Exoenzymen eingesetzt wird, weil es hohe Proteinmengen sekretieren kann. Das Genom wurde 2004 im Göttinger Genomlabor sequenziert. Seit der …