Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 21 for “"DNA transfer"”.
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Liposome-Mediated DNA Transfer
… Swine oocytes were incubated with liposome/DNA treated sperm and were examined for male pronuclear development. The results for three experiments indicate that 93.9% of the oocytes underwent maturation (81.7% for controls), 84.8% of the matured oocytes were penetrated (90.0% for controls) …
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The DNA Transfer Region of Bacteroides Conjugative Transposons Tc(r)Em(r)DOT and Tc(r)ERL
… the first detailed study of the conjugal transfer genes found on a conjugative transposon. The complete sequence of a functional transfer region is presented and comparative analysis is made between the transfer gene sequences of several conjugative transposons. Two of the newly discovered …
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Characterization of TraM, a Required Bacteroides Fragilis Conjugal Apparatus Protein That Interacts with the B. Fragilis Putative Coupling Protein Bcta and the B. Fragilis Relaxase Protein BmpH
… antibiotic resistance genes which promiscuously transfer within and from the genus via conjugation. The <italic>B. fragilis</italic> clinical isolate LV23 harbors a 37kb conjugative transposon, BTF-37. Two important conjugal apparatus proteins encoded by BTF-37 are BctA, a predicted coupling …
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The use of the broad-host-range plasmid, pTF-FC2, in Agrobacterium tumefaciens mediated plant transformation
The transfer of T-DNA from Agrobacterium tumefaciens to eukaryotic cells is on of the most extensively studied examples of interkingdom DNA transfer. This transfer system has revolutionized plant molecular biology to the extent that the introduction of foreign genes into plant genomes is now a …
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Construction of inducible expression systems for controlled transfer of Agrobacterium tumefaciens T-DNA in plant transient expression systems /
… that provides for strictly regulated T-DNA transfer from agrobacteria to model plants Nicotiana benthamiana. The study provided detailed information about the relative activity and regulation of several IPTG and cumic acid inducible promoters in A. tumefaciens. Additionally, new …
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Conjugation of extrachromosomal replicons of Rhodococcus erythropolis AN12
… encoded by the traA gene is required to initiate DNA transfer during plasmid conjugation. Sequences adjacent to the transposon insertion in megaplasmid pREA400 revealed a putative traA-like open reading frame. A novel site-specific gene disruption method was developed to generate a traA mutation …
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An experimental investigation into the mechanisms of bacterial evolution
… of bacterial evolution — horizontal gene transfer and spontaneous mutation, in the bacterium <em>Escherichia coli</em> through novel experimental assays and mathematical simulations. First, I will develop a growth assay utilizing the quantitative polymerase chain reaction (qPCR) to provide …
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Lateral Transfer of DNA in Protocell-like Synthetic Cells
… whether electroporation can mediate the transfer of DNA into and between synthetic cells, potentially enabling gene expression in initially DNA-free compartments. Both TXTL and PURE-based cell-free expression systems were encapsulated in lipid in order to evaluate fluorescence as a proxy …
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Characterization of The Role of The Pkm101-Encoded T4Ss Outer Membrane Core Complex In Substrate Transfer, Pilus Biogenesis, and Recipient Cell Contact
… to study the role of the AP cap in (i) substrate transfer to recipient plant or bacterial cells, (ii) pilus biogenesis and donor-specific bacteriophage sensitivity, and (iii) recipient cell contact. My results demonstrate that the C-terminal tails of VirB10 and TraF<sub>pKM101</sub> are essential …
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The development of Sinorhizobium meliloti and Deinococcus radiodurans as chassis for synthetic biology applications
… as a novel conjugative host and demonstrated the transfer of multi-host shuttle vectors to bacteria, yeast and microalgae. Then, I developed a conjugative protocol to transfer DNA from E. coli to D. radiodurans and showed its utility through the generation of robust systems for conjugation-based …
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Development of a Gene Transfer System in Clostridium Scindens VPI 12708
… due, in part, to a lack of a functional genetic transfer system for this strain. A spontaneous rifampinresistant Clostridium scindens VPI 12708 mutant was used as a recipient to determine the efficacy of conjugation as a method of DNA transfer. The Clostridium perfringens plasmid pECU-001, …
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Transfer functions of Streptomyces plasmid pIJ101: insights into a novel bacterial conjugation system
For Streptomyces plasmid pIJ101 to mediate DNA transfer, only the clt locus and the Tra protein are required, though three non-essential spread functions increase the radial distance of plasmid dissemination among recipients after the initial lateral transfer event. clt is a contiguous 54-bp …
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Deoxyribonucleic acid restriction/modification systems and gene transfer strategies in Ruminococcus albus 8 and Ruminococcus flavefaciens FD-1
One goal critical to the use of recombinant DNA technologies with rumen bacteria is the establishment of a stable DNA transfer system. Research to date has shown that Ruminococcus can be classified as a genus resistant to transformation, and that electroporation may offer the only means to …
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Towards plastid transformation in rapeseed (Brassica napus L.) and sugarbeet (Beta vulgaris L.)
… proven by PCR analysis, but an integration of DNA into the plastome could not be verified so far. Efficient regeneration systems and methods of DNA transfer were established for rapeseed and sugarbeet and straightened the way for successful plastid transformation in either species.
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Zur Vielfalt allgemein transduzierender Bakteriophagen in bakteriellen Gemeinschaften sowie innerhalb der Spezies Salmonella enterica serov. typhimurium
Die Rolle der Transduktion beim horizontalen Gentransfer ist bislang wenig untersucht. Die Bedeutung, die diesem Mechanismus beim Austausch von genetischem Material in der Natur zukommt, kann nur geklärt werden, wenn möglichst umfassende Informationen über das Vorkommen von transduzierenden …
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Developmental genetic studies on Thiobacillus ferrooxidans
… Conjugation was attempted as a means for plasmid DNA transfer to T. ferrooxidans. Recombinant T. ferrooxidans plasmids, pDER401 and pDER405, were shown to code for mobilization and replication functions in Escherichia coli and Thiobacillus novellus strains. The plasmids were mobilizable at high …
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Expression of atrial natriuretic factor in transgenic potatoes
… (GUS) to confirm the functionality of the DNA regulatory sequences used. All six chimaeric gene fusions were cloned into the binary vector, pGA643, or its derivative. These were then introduced into potato plants via leaf disc transformation using Agrobacterium tumefaciens strain LBA4404 …
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Understanding angiosperm genome interactions and evolution: insights from sacred lotus (Nelumbo nucifera) and the carrot family (Apiaceae)
Horizontal and intracellular gene transfers are driving forces in plant evolution. The transfer of DNA into a genome adds genetic diversity and successfully incorporated genes can retain their original function or develop new functions through mutation. While there are trends and hypotheses for the …
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