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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 210 for “"DNA synthesis"”.
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Discontinuous DNA synthesis in mammalian cells.
Thesis. 1976. Ph.D.--Massachusetts Institute of Technology. Dept. of Biology.
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Viral Inhibition of Mammalian Cell DNA Synthesis
<p>Eukaryotic cells replicate their DNA during a defined time period in the interphase between mitoses. Non-proliferating cells are generally found to be blocked in interphase prior to the DNA-synthetic period. It therefore appears that the control of cellular proliferation may be intimately …
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Photoelectrochemical array platform for genomic scale DNA synthesis
… for large, high-fidelity constructs of synthetic DNA. Recent developments in harvesting oligonucleotides from DNA microarrays has proven that these can be assembled into large constructs of DNA for costs significantly less than traditional methods. This thesis presents a complete platform for the …
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DNA synthesis and methylation in normal and transformed cells
In this study, DNA methylation was examined during the eukaryotic cell cycle, and shown to occur throughout the S phase as well as during the "early" G₂ phase. However, DNA synthesis and methylation of newly synthesized DNA did not occur simultaneously, but the latter lagged behind DNA synthesis by …
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Mitigating security issues in the evolving DNA synthesis industry
DNA synthesis technologies are advancing at exponential rates, with production of ever longer, more complex, and less expensive sequences of double stranded DNA. This has fostered development of industrial scale design, construction, and sale of synthetic DNA. The tools and methods of synthesis …
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The effect of oxidized guanines on DNA synthesis by DNA polymerase β
DNA replication is the fundamental biological task of duplicating genetic information for passing on to a daughter cell. DNA polymerases are the molecular machines that read the parent DNA strand and instruct the addition of correct corresponding nucleotides (also termed fidelity) to form a new DNA …
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Structure-Guided Design of Novel Therapeutics Targeting Translesion DNA Synthesis and Lipid A Biosynthesis
… cases occurring in the US alone each year. DNA-damaging agents are often the first line of defense against rapidly dividing cancer cells. However, cancer cells can become resistant to chemotherapy by up-regulating an error-prone DNA-repair process called translesion DNA synthesis (TLS). The …
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Novel regulatory mechanisms of mutagenic translesion DNA synthesis : characterization of REV1 in Saccharomyces cerevisiae
Cells are constantly subjected to DNA damage from endogenous and exogenous sources. Spontaneous DNA damage alone accounts for -30,000 DNA lesions per day in a mammalian cell. Cells are also exposed to an enormous variety of environmental agents that can cause a wide range of modified bases and …
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Vitamin B6 Decreases Proliferation and DNA Synthesis in Human Mammary Carcinoma Cell Lines In Vitro
… (ER) by blocking the hormone-binding and/or DNA-binding site of the ER. The objective of this study was to examine the effects of Vitamin B6 supplementation on cell proliferation and estrogen-dependent gene expression in breast cancer cells. To accomplish this, estrogen-dependent (MCF-7 and …
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Role and regulation of prostaglandin production in granulosa cell DNA synthesis during ovarian follicular development.
… by hydroxyurea (1.5-6 mM). TGF$\alpha$-induced DNA synthesis in F1 and F5-6 granulosa cells was suppressed by inhibitors of phospholipase A$\sb2$ and cyclooxygenase, while an inhibitor of lipoxygenase was ineffective. The inhibition was concentration-dependent and could be attenuated by …
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Biochemical and Genetic Studies on Origin Recognition and DNA Synthesis From the Mesophilic Archaeon Methanosarcina Acetivorans
… that DinB was able to synthesize through abasic DNA and cyclobutane pyrimidine dimers, and these activities were stimulated by the cognate PCNA. Fascinatingly, unlike most translesion DNA polymerases previously described, MacDinB synthesized an unusually long product (∼7.2 kb) in the presence …
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In Vivo Studies of DNA Synthesis in Escherichia Coli Are Consistent With a Discontinuous Model of DNA Replication
During our research we also found that the identification of replication intermediates in some cases has been mistaken by the presence of polyphosphate species. We show evidence that these polyphosphate species are not replication intermediates, and we go further into developing a simple method to …
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Interaction of Putative Estrogens and the Estrogen Receptor System in Leydig Cells in the BALB/c Mouse Testis Resulting in the Initiation of DNA Synthesis
… incorporation of <sup>3</sup>H-tymidine into the DNA of the interstitial cells. These effects are hypothesized to be mediated by the estrogen receptor system in the Leydig cell. Common experimental techniques for the measurement of hormone binding, such as dextran coated charcoal treatment, proved …
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THE REGULATION OF POLYAMINES AND THEIR ROLE IN THE PROLIFERATION OF HELA CELLS
… cells which were synchronized for DNA synthesis in the presence of 1,3-diamino-2-hydroxypropane (1.0 mM) and (alpha)-difluoromethylornithine (1.0 mM) exhibited a severe deficiency in S phase DNA synthesis and cell proliferation. This deficiency was reversible in that exogenous …
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Nuclear and Plasma Membrane Properties of Macrophage Heterokaryons and Hybrids
… peritoneal macrophages, which do not synthesize DNA in vitro, were fused with a strain of mouse melanoma cells which proliferates rapidly in vitro. The plasma membrane of the macrophage has specific receptors which enable the cell to ingest antibody-coated sheep red cells and is also rich in a …
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Functional Role of Hepatitis B Virus Core Protein in Viral Replication
… HBV pregenomic RNA (pgRNA) encapsidation and DNA synthesis: 40% amino acid sequence identity or 45% homology in the nucleic-acid binding domain of HBV core protein was sufficient for pgRNA encapsidation and DNA synthesis, although we predominantly detected spliced DNA. A chimeric core protein …
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High throughput single molecule in situ-verified nucleic acid synthesis
… in computer programs, and specified at the DNA level. Subsequently, DNA encoding the design must be built to specification and tested for desired functionality in vivo or in vitro. In current practice, building DNA, by de novo DNA synthesis and related methods, is a rate limiting and costly …
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