Global ETD Search
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Showing 1 to 12 of 12 for “"DNA supercoiling"”.
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DNA supercoiling with a twist
The level of torsion in double-stranded DNA regulates base-pair stability and DNA conformation. It is important in initiation and regulation of specific DNA metabolic processes as well as chromatin assembly. High mobility group proteins (HMGB) are architectural proteins whose HMG DNA binding …
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Analysis of the structural changes caused by positive DNA supercoiling
The procession of helix-tracking enzymes along a DNA molecule results in the formation of supercoils in the DNA, with positive supercoiling (overwinding) generated ahead of the enzyme, and negative supercoiling (underwinding) in its wake. While the structural and physiological consequences of …
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Genome-Wide and Multi-Scale Mapping of DNA Supercoiling in Differentiating Human Stem Cells
… levels to accommodate the two-meter-long DNA within the confined nuclear space of six microns. Chromatin folding is not only a cellular need, but is also crucial for gene regulation, and coordinates the precise rewiring of gene expression programmes in every cell type during developmental …
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The role of DNA supercoiling in the coordinated regulation of gene expression in Helicobacter pylori
… characterized yet. Changes in global or local supercoiling of chromosomal DNA are thought to play a role in global gene silencing and gene activation. In Helicobacter pylori, a bacterium with few dedicated transcriptional regulators, the structure of some promoters indicates a dependency on DNA …
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Profiling hotspots of DNA breaks and learning-induced gene expression in the mouse brain
Neuronal activity generates DNA double-strand breaks (DSBs) in the brain. Topoisomerase IIb (TOP2B), important for relieving transcription-associated DNA supercoiling, was implicated as the source of these neuronal activity-induced DSBs, facilitating rapid transcriptional induction of immediate …
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Interactions between nucleoid associated proteins and DNA in the presence of mechanical and chemical forces
"The way that DNA is organized within a cell controls its physiological behavior. DNA must be condensed in order to fit into the much smaller cell, but must also be accessible to proteins responsible for biological processes. Architectural proteins assist with this large-scale arrangement of DNA to …
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The physics of cell-fate choice
… in cell-fate choice--- a mechanical model of DNA supercoiling-mediated transcriptional noise and a coarse-grained model of noise in partitioning during cell division that can create and maintain a phenotypically heterogeneous population. Finally, I describe a mechanistic model of the key …
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MED9, a Mediator Complex Component, and its Interacting Protein MORC1 Balance Growth and Defense in Arabidopsis
… is also a topoisomerase that tightens chromatin/DNA supercoiling. I identified 14 MORC1-interacting proteins (MIPs) via yeast 2-hybrid screening, eight of which have putative nuclear-associated functions. While a few loss-of-function mutants for these MIPs displayed altered bacterial resistance …
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Investigating the mitotic roles of Topoisomerase 2 Alpha and Topoisomerase 2 Beta-Binding Protein 1 in the maintenance of chromosome structure and genome stability
… act by generating a transient double-stranded DNA break in one molecule and manoeuvring a second DNA segment through the resultant gap. This ability to catenate and decatenate DNA means that type II topoisomerases are important not only in relieving DNA supercoiling during DNA transcription, …
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EFFECT OF MISMATCHED BASE PAIRS ON DNA PLECTONEMES
Base pair mismatches in DNA occur during replication and can result in mutations and certain types of cancer. The exact mechanism by which mismatch repair proteins recognize mismatches is still not well understood. Structures of mismatch recognition proteins bound to a mismatch indicate that the …
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Non-equilibrium physics of driven living matter
The student, Purba Chatterjee, submitted this Dissertation for approval on 2021-05-07 at 09:11.
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Uncovering pathways necessary for the survival of RNase H-deficient Escherichia coli
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2026-05-01