Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 36 for “"DNA structures"”.
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Probing Non-Canonical DNA Structures With Anticancer Drugs
… aided by studying the structural perturbation of DNA caused by T:G base pair and by specific probing of the mismatch using small molecular ligands. We have shown that AR-1-144, a tri-imidazole minor groove binder, recognizes the CCGG sequence. The NMR structural analysis of the symmetric 2:1 …
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Influence of Kaempferol on Noncanonical DNA Structures and Protein Aggregation
… that single and double stranded nucleic acid structures can serve as receptors for flavonoids. Noncanonical DNA motifs (such as C<sub>4</sub> and G<sub>4</sub> tetraplexes) are commonly found in human telomeres and protooncogenes, which can serve as potential targets to treat cancer. …
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Re-evaluation of analytical chemistry techniques in studying DNA structures
… to examine the structural changes that DNA adopts when subjected to a number of external/internal factors. A self-complementary sequence, d(CG)9, and a non-self-complementary sequence (mixed sequence) were used to study the conformational effects displayed by each type of oligonucleotide …
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Sequence Effects and Ligand Selectivity in Targeting Non-Canonical DNA Structures
G-Quadruplex (G4) DNA is a secondary DNA structure that can occur in G-rich DNA sequences. These structures, which are compact and diverse, have been implicated in a wide range of processes including telomere maintenance, transcriptional control, and DNA replication and repair. In order to target …
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Defining the Role of Secondary DNA Structures and Transcription Factors on the Transcriptional Control of the HIF-1alpha and VEGF Promoters
… undergo a conformational transition between B-DNA, single stranded DNA and atypical secondary DNA structures such as G-quadruplexes and i-motifs. We hypothesize that the i-motif and G-quadruplex structures can form within the pPu/pPy tracts of the HIF-1α and VEGF proximal promoters, which play …
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Molecular studies of homologous chromosome pairing in Triticum aestivum / by Stephen W. Thomas.
This thesis identifies DNA structures and genes involved in the process of homologous chromosome pairing in allohexaploid bread wheat (Triticum aestivum). In addition to studying late replicating DNA, a speculative model on the action of the pairing genes in allohexaploid wheat and the putative …
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Single molecule studies of helicase mechanisms
… canonical helicase function of unwinding duplex DNA, the RecQ family of helicases is known to preferentially bind to non-canonical DNA structures such as G-quadruplexes and Holliday junctions. Ensemble biochemical research has shown that BLM probably has roles in the maintenance of genome …
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DNA based networks, nanostructures, and meta-stable states
… important materials is deoxyribonucleic acid (DNA). DNA has several unique properties that make it a good candidate for nanotechnology research. The primary aim of this research is to use DNA three-way junctions as building blocks and to assemble them into a huge DNA networks through self …
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Flap Endonuclease 1 ensures telomere replication and stability
Telomeres, protein-DNA structures that distinguish the end of a chromosome from a bona fide DNA double strand break, are integral to genomic stability. High fidelity replication of telomeres is indispensable for their stability. Telomere replication is a challenging task that is completed through …
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Interactions of small molecules with duplex DNA and lesion containing G-quadruplex DNA
… to study their interactions with duplex DNA. The nickel complexes were found to selectively oxidize guanines in bulged DNA structures in the presence of oxidant and notably NiCR-2H oxidizes guanines more efficiently than NiCR. According to 1 H NMR studies, NiCR-2H binds strongly to the N7 …
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Structural determinants of mutability across cancer genomes
… of this variation. Here the role of alternative DNA structures was investigated across a multitude of whole-genome sequenced cancers. Sequences that are predisposed to fold in alternative DNA structures can be identified by the primary DNA sequence of the human genome and are collectively known …
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Structure of the amplified 5-enolpyruvylshikimate-3-phosphate synthase gene in glyphosate-resistant carrot cells and attempts to isolate the anthranilate synthase gene from plants
… synthase (EPSPS) DNA of carrot suspension cultured cell lines selected for glyphosate resistance was carried out to determine the mechanism of gene amplification in this plant system. Cloning of the EPSPS gene and 5$\sp\prime$ flanking sequences was carried out and …
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Novel regulatory mechanisms of mutagenic translesion DNA synthesis : characterization of REV1 in Saccharomyces cerevisiae
Cells are constantly subjected to DNA damage from endogenous and exogenous sources. Spontaneous DNA damage alone accounts for -30,000 DNA lesions per day in a mammalian cell. Cells are also exposed to an enormous variety of environmental agents that can cause a wide range of modified bases and …
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Synthesis and use of new Porphyrin Derivatives
… interact with the anionic backbones of different DNA structures, displaying a different protonation degree depending on pH. The results obtained by CD-measurements, UV-Vis, fluorescence and RLS spectroscopy support the good ability of these derivatives (i.e. ZnTCPPSpm4) to act like inducer, probe …
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The Biogenesis of Small Interfering RNA in Neurospora Crassa
… termed quelling; the other type is induced by DNA damage and functions to slow down protein translation after DNA damage. Both of these siRNAs originate from repetitive sequences in the Neurospora genome. We show that the components of the homologous recombination (HR) machinery are required to …
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G-wires: The growth and characterization of a G4-DNA nanostructure
… of forming G-quartets are classified as G-DNA. The family of G-DNA structures includes anti-parallel hairpin dimer conformations (G'2-DNA) and parallel tetramer conformations (G4-DNA). In this work we have employed the techniques of gel electrophoresis, UV spectroscopy and atomic force …
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REVERSIBLE QUINONE METHIDE ALKYLATION OF DNA
Alkylation of DNA has been found to cause cancer and also to serve as its treatment. Quinone methides (QMs) are highly electrophilic molecules implicated in numerous metabolism processes. Studies of QM's reversible reaction with nucleophiles of DNA are important to understand the mechanism of its …
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Multi-Level Coarse-Grained Modelling on DNA Functionalised Building Blocks
DNA nanotechnology has proliferated in recent years as investigators have increasingly harnessed the selectivity of DNA base pairing to form multitudes of diverse structures, with a wide range of applications. The stiffness of the double-strand DNA (dsDNA) with a persistence length of around 50nm …
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Exciton Dynamics in DNA-Chromophore Assemblies
… mimicking these nat- ural architectures, we use DNA-chromophore assemblies to generate excitonic circuits with nanometer-scale precision over chromophore placement and orientation. First, in a bioinspired light-harvesting system, we explore the role of molecular parameters in directing electronic …
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G-Triple Chemiluminescent DNAZyme Based Biosensor for the Detection of Calcium(II) Ions in Solution and the Biological Functionality of Kinase Enzymes and the Rationale for their Employment as Promising Drug Targets, Substrate Specific Inhibition of P38alpha
G-quadruplex DNA structures, which are formed from planar stacks of G-tetrads and follow Hoogsteen hydrogen bonding, have recently received attention as recognition elements in bioanalytical assays. DNAzymes are functionally active, synthetic single-stranded DNA molecules that are capable of …
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