Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 9 of 9 for “"DNA shuffling"”.
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Molecular Breeding of Porcine Circovirus Type 2 by Synthetic DNA Shuffling
… is a small, non-enveloped, single-stranded DNA virus that causes disease in pigs and is an economically important pathogen affecting pig populations worldwide. PCV2 contains two major open reading frames (ORF): ORF1 encodes two replicase proteins and ORF2 encodes the immunogenic capsid …
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Mutation analyses of N-terminal of brome mosaic virus polymerase-like protein by deletions, point mutations and DNA shuffling: effects on polymerase-helicase interaction
This document only includes an excerpt of the corresponding thesis or dissertation. To request a digital scan of the full text, please contact the Ruth Lilly Medical Library's Interlibrary Loan Department (rlmlill@iu.edu).
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Massively parallel analysis of the functional effects of mutations
… large libraries of chimeric sequences using DNA shuffling, and applied the method to yeast flocculation. Finally, in Chapter 6 I discuss some of the outstanding questions for massively parallel assays, including research directions that I believe will be important in the future.
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Protein and Pathway Engineering for Biosynthesis or Aromatic Compounds
… to improve the poor properties of PhlD. Family shuffling of 52 PhlD homologous genes using synthetic DNA shuffling technique gave two improved PhlD mutants, which showed 4 fold increased enzymatic catalytic efficiency and/or thermostability. Secondly, the availability of substrate for …
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Engineering Estrogen Receptor-Based Gene Switches and a Superoxide Dismutase for Therapeutic Applications
… approach to directed evolution. This method---DNA shuffling with spiked oligonucleotides (DSSO)---simultaneously randomizes multiple functionally important sites in a protein."
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Estrogen Receptor Subtype Selective Ligands
… we generated a series of ER chimeras using DNA shuffling. We found that the ligand-binding domain (LBD) of ER was the major determinant of the subtype-selective character of the ligands, and that the structural basis of the selectivity for different groups of ER subtype-selective ligands was …
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Reverse Genetics-based Approaches to Attenuate Porcine Reproductive and Respiratory Syndrome Virus (PRRSV)
… and study of PRRSV genes, we first established a DNA-launched infectious clone of the passage 14 PRRSV strain VR2385, pIR-VR2385-CA, and identified a spontaneous 435-bp deletion in the nsp2 gene. To characterize the biological and pathological significance of this nsp2 deletion, we restored …
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Engineering, overexpression, purification and analytical applications of recombinant bioluminescent reporters.
… was accomplished through repeated rounds of DNA shuffling, cloning and expression of mutant proteins, followed by screening and selection of regenerated mutants. Increased ratios of luminescent signals to the mass of total soluble protein were compared as criteria for mutant selection after …
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Genetic engineering of antibody fragments for the detection of illicit drugs
… strategies exploited error prone-PCR, DNA shuffling, light-chain shuffling and various methods of site-directed mutagenesis to generate improved affinity antibody fragments. Kunklestyle, site-directed mutagenesis, proved to be the most successful method for the generation of an anti-M3G …