Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 340 for “"DNA sequencing"”.
-
Nanopore technology for DNA sequencing
Made available in DSpace on 2010-08-20T17:58:37Z (GMT). No. of bitstreams: 3 Comer_Jeffrey.zip: 226477214 bytes, checksum: 58e732aa747f3bd4da0e576ddcc65779 (MD5) Comer_Jeffrey.pdf: 19129383 bytes, checksum: be143050e3104cb300d385ce2da0c51e (MD5) license.txt: 4062 bytes, checksum: …
-
Signal processing for DNA sequencing
DNA sequencing is the process of determining the sequence of chemical bases in a particular DNA molecule-nature's blueprint of how life works. The advancement of biological science in has created a vast demand for sequencing methods, which needs to be addressed by automated equipment. This thesis …
-
Cell-Free Dna Sequencing In Multiple Myeloma
… both in acute and long-term settings. Cell-free DNA, DNA fragments which are released into circulation as a part of normal cellular turnover, is a useful and dynamic biomarker in cancer patients due to the presence of circulating tumor DNA (ctDNA), which is readily identified using next …
-
Scheduling of biological samples for DNA sequencing
In a DNA sequencing workflow, a biological sample has to pass through multiple process steps. Two consecutive steps are hydroshearing and library construction. Samples arrive randomly into the inventory and are to complete both processes before their due dates. The research project is to decide the …
-
Error Correction in Next Generation DNA Sequencing Data
Motivation: High throughput Next Generation Sequencing (NGS) technologies can sequence the genome of a species quickly and cheaply. Errors that are introduced by NGS technologies limit the full potential of the applications that rely on their data. Current techniques used to correct these errors …
-
Expanding the applications of high-throughput DNA sequencing
DNA sequencing is the process of determining the identities of the nucleotides that make up a molecule of DNA. The rapid pace of advancements in sequencing technologies in recent years have made it possible to simultaneously determine the sequences of hundreds of millions of short DNA fragments. …
-
Genome scanning : an AFM-based DNA sequencing technique
Genome Scanning is a powerful new technique for DNA sequencing. The method presented in this thesis uses an atomic force microscope with a functionalized cantilever tip to sequence single stranded DNA immobilized to a mica surface. The functionalized cantilever tip hybridizes with only one base …
-
Novel molecular engineering approaches for genotyping and DNA sequencing
… (MALDI-TOF MS) offers an attractive option for DNA analysis due to its high accuracy, sensitivity and speed. In the first part of the thesis, we report the design, synthesis and evaluation of a novel set of mass tagged, cleavable biotinylated dideoxynucleotides (ddNTP-N3-biotins) for DNA …
-
Computational optimisation strategies for targeted DNA sequencing using nanopores
Long-read DNA sequencing is causing a generational shift in genome sequencing productivity and is revolutionising many aspects of biological discovery. One of the technologies behind this transformation is sequencing using nanopores that act as biosensors measuring fluctuations of an ionic current …
-
Application of manufacturing tools in the DNA sequencing process
Thesis (M.B.A.)--Massachusetts Institute of Technology, Sloan School of Management; and, (S.M.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering; in conjunction with the Leaders for Manufacturing Program at MIT, 1999.
-
Measuring ongoing chromosomal instability in single-cell DNA sequencing data
… replication status. Cancer cells often exhibit DNA copy number aberrations and can vary widely in their ploidy as a consequence. Correct estimation of the ploidy of single cell genomes is crucial for many aspects of downstream analysis, such as copy number calling and inference of cell …
-
Development of a microfluidic platform for integrated DNA sequencing protocols
… reduce costs and improve the quality of in the DNA sequencing methodology currently implemented at the Broad Institute in Cambridge, Massachusetts. The Sequencing Center at the Broad Institute currently generates an average of 130 million bases per day with an average read length of 800. This is …
-
Optimization of data acquisition system for novel DNA sequencing instrument
Thesis (S.B. and M.Eng.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2000.
-
Return on investment and library complexity analysis for DNA sequencing
… the profiles of information acquisition during DNA sequencing experiments is critical to the design and implementation of large-scale studies in medical and population genetics. One known technical challenge and cost driver in next-generation sequencing data is the occurrence of non-independent …
-
An analysis of the next generation DNA sequencing technology market
… the ongoing development of the next generation DNA sequencing market in an effort to predict exactly which factors will play a role in determining who will ultimately succeed. This will be accomplished through an analysis incorporating a combination of historical precedents in this industry and …
-
Error Correction and de novo Genome Assembly of DNA Sequencing Data
… sciences. Current technologies are capable of sequencing short pieces of DNA with very high quality. These short pieces of DNA determint the sequence of bases in the genome of any species. This information is key in understanding many of the aspects of how life functions. The accuracy of …
-
Integrated Analog Readout Array and Digital Backend for Mobile DNA Sequencing
DNA, a fundamental biomolecule, contains the genetic code that governs the development, functioning, and reproduction of all living organisms. It is composed of smaller molecular units called nucleotides. The process of determining the specific sequence of these nucleotides is known as DNA …
-
Investigating Metastatic Lineage In Colorectal Cancer By Single Cell Dna Sequencing
… have applied conventional bulk next-generation sequencing (NGS) methods, which have limited ability to resolve intratumor heterogeneity.</p> <p>To address this problem, we have developed a highly-multiplexed single cell DNA sequencing method that combines flow-sorting of single nuclei, …
-
Statistical Methods For Resolving Intratumor Heterogeneity With Single-Cell Dna Sequencing
… and treatment resistance. Single-cell DNA sequencing is a promising experimental method for studying intratumor heterogeneity, but brings unique statistical challenges in interpreting the resulting data. Researchers lack methods to determine whether sufficiently many cells have been …
-
Elucidating aquatic virioplankton diversity and dynamics using high-throughput DNA sequencing
… ecology was the application of high-throughput DNA sequencing to entire viral communities. Known as shotgun viral metagenomics, this approach allows access to the majority of viruses that cannot be maintained in culture. Viral metagenomics has revealed surprising insight into ancient …
Page 1 of 17