Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 27 for “"DNA sensing"”.
-
Graphene supported hafnium oxide nanopores for DNA sensing
… fronts including surface charge engineering for DNA capture and conductance modulation in nanopores, nanowire transistors for localized detection, ultra-thin membrane fabrication using graphene, and the exploration of alternative nanopore materials for biosensing applications. In this work, the …
-
Computational study of graphene nanopore sensor for DNA sensing
… to the physical dimension, of the translocating DNA molecule, that can be resolved. Typical solid-state membranes are too thick and usually fail to recognize single nucleotides on a DNA strand. Graphene is a sub-nanometer membrane, comprising of carbon atoms arranged in a honeycomb lattice, with …
-
Investigating HSV-1 pUL56 mediated antagonisation of DNA sensing mechanisms
… Simplex Virus 1 (HSV-1) is a large, enveloped DNA virus that infects around two-thirds of the world population. HSV-1 is known to successfully remodel the host cell microenvironment to allow for efficient replication and propagation of progeny virions. One of the ways in which HSV-1 achieves …
-
Mechanisms of cytosolic DNA sensing and neuroinflammation in ataxia-telangiectasia
… mutations in genes critical for the DNA damage response. These disorders commonly present with neurological disease, exemplifying the importance of preserving genome stability in the central nervous system (CNS). Ataxia-telangiectasia (A-T) is a prototypical genome instability …
-
Liquid-Liquid Phase Separations in Innate Immune DNA Sensing and NF-κB Signaling Pathways
The binding of DNA to cyclic GMP-AMP synthase (cGAS) leads to the production of the secondary messenger cyclic GMP-AMP (cGAMP), which activates innate immune responses. We have shown that DNA binding to cGAS robustly induced the formation of liquidlike droplets in which cGAS was activated. The …
-
Mechanisms of Microbial DNA Sensing by the AIM2 Inflammasome and Structural Study of Polynucleotydyl Transferase1 (pnt1) from Arabidopsis Thaliana
… 2) can sense foreign cytosolic double-stranded DNA (dsDNA) through the dsDNA binding HIN-200 domain at the C-terminus. Once dsDNA is bound to HIN-200 domain, AIM2 can activate the AIM2 inflammasome, resulting in maturation of pro-interleukin-1? by activation of caspase-1. To investigate the …
-
Stability and bandwidth investigation of alternative structures for nanopore sensors
The genetic information carriers, DNA molecules can be thought of as the blueprints of living organisms. This crucial functionality of the DNA mole- cules may explain the drive and momentum for DNA sequencing research. The commonly used parallel sequencing methods require extensive sample …
-
Sensing Foreign DNA: The Role of DNA-PKcs in Human Anti-viral Innate Immunity
… patterns (PAMPs), such as viral genomic DNA, which is a major PAMP during DNA virus infection. Viral DNA sensing leads to the activation of the STING-TBK1-IRF3 signalling axis and the production of type I interferon. Previously, our work identified the non-homologous end-joining protein …
-
An examination of the role of IFI16 in detecting viral DNA in human immortalised keratinocytes
The presence of exogenous DNA in the cytosol results in the activation of the DNA sensor cyclic GMP-AMP synthase (cGAS). cGAS produces the second messenger cyclic GMP-AMP (cGAMP) which binds to and activates the endoplasmic reticulum adapter STimulator of INterferon Genes (STING). Activated STING …
-
CMOS nanofluidics
… part of clinical diagnosis involves protein and DNA sensing. Significant effort is made to make protein and DNA sensing more accessible and affordable, through micro and nano-technologies. However, typical commercial and academic devices for molecular sensing suffered needs for external …
-
Role of the Innate Immune Response to DNA During Therapy with Oncolytic Viruses
… killing virus (SKV), through cytoplasmic DNA sensing pathways and how manipulation of this pathway may impact OV efficacy. Here, I screened several syngeneic murine cancer cell lines for their expression of cytoplasmic DNA sensing proteins and susceptibility to OV infection. After …
-
Innate Immune Sensing and Signaling of Cytosolic DNA
In eukaryotic cells, DNA is normally confined within the nucleus and mitochondria. DNA exposed in the cytosol is a danger signal that warns the host of invading microbial pathogens and triggers innate immune responses including the production of type-I interferons (IFNs). Endogenous DNA that is …
-
The cell-intrinsic innate immune response to DNA damage in human cells
The innate immune response has evolved to detect DNA from viruses and intracellular bacteria and differentiate this from our almost identical self-DNA. DNA detected in the cytoplasm is thought to be identified as foreign due to its location, and bacterial DNA in endosomes in recognised by its …
-
Cell-free synthetic biology for affordable, on-demand diagnostics
… detection technology to create a set of fruit DNA-sensing demonstrations that can be used in high school biology classrooms. I then sought to engineer biomolecular circuits that can process multiple sensor inputs to reduce cost, improve specificity, and build classifier circuits. Finally, I …
-
Intratumoral Sting Activation Sensitizes Poorly Immunogenic Cancers to Checkpoint Blockade Immunotherapy
… inflammatory signaling through the cytosolic DNA sensing pathway component stimulator of interferon genes (STING) could reprogram tumor associated macrophages (TAMs) and myeloid derived suppressor cells (MDSCs) to support rather than suppress T cell activity, converting CRPC and PDAC to …
-
Sensing with Nanomaterials: Controlling Transport, Single Molecule Detection, and Voltage Sensing
… disruptive membranes for ionic selectivity and sensing based applications. Atomic thinness of these materials affords a unique environment in an all-surface material to unlock challenges towards improving desalination, energy harvesting and DNA sensing. Within this thesis the primary focus will …
-
Toward the Rational Design of Better Antivirals: The Development of cGAMP as an HIV-1 Anti-Retroviral and the Genetic Surveillance of WNV Evolution
… immune detection by the cGAS-STING cytosolic DNA sensing pathway. A small cyclic di-nucleotide, cGAMP, activates the same pathway by directly binding STING. Treatment with cGAMP, delivered by ultra-pH sensitive nanoparticles or by liposomes, in human peripheral blood mononuclear cells (PBMCs) …
-
Advances in Microfluidics and DNA Nanotechnology for Biomolecular Analysis
The sensing and characterisation of biomolecules is crucial for medical diagnostics and an understanding of biological systems. Proteins play a leading role in biology, by performing the majority of biochemical and biophysical processes that maintain homeostasis. As such, they and their …
-
Biological, Clinical, and Functional Implications of Cell-Free DNA Topology in Cancer
Cells can release their DNA into the extracellular space and circulation by active mechanisms or as a product of cell death. In oncology, this cell-free DNA (cfDNA) has emerged as a promising source of cancer biomarkers because it contains actionable information about a patient’s disease and can be …
-
Investigations into the vaccinia virus immunomodulatory proteins C4 and C16
… has two reported functions. Firstly, it inhibits DNA-PK-mediated DNA sensing, and this study found that C4 can perform this function as well. Like C16, C4 associates with the Ku heterodimer to block its binding to DNA leading to reduced production of cytokines and chemokines. For both proteins, …
Page 1 of 2