Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 22 for “"DNA replication initiation"”.
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Developmental regulation of DNA replication initiation in Drosophila
… a powerful system for the study of metazoan DNA replication. Amplification produces 100kb gradients of amplified DNA through repeated rounds of origin firing and bidirectional movement of replication forks from these origins. The Drosophila Follicle Cell Amplicon at the cytological location …
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Mechanism and importance of Mcm2-7 double-hexamer formation during DNA replication initiation
… and accurately in each cell cycle. Thus, DNA replication is a highly-regulated multi-step process that ensures the genome is duplicated only once per cell cycle. In eukaryotic cells, initiation of DNA replication begins with loading of two heterohexameric Mcm2-7 helicases around origin DNA …
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Analysis of metazoan DNA replication initiation using Drosophila gene amplification as a model system
… specification and developmental regulation of DNA replication in vivo. We mapped all follicle cell amplicons using a comparative genomic hybridization strategy and identified two new amplicons. We determined the precise localization of the origin recognition complex (ORC) on a genome-wide level …
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Delineation of the molecular mechanisms underlying DNA replication initiation and changes in gene copy number during Drosophila development
The study of differential DNA replication programs in Drosophila has provided important insight into the molecular control of replication initiation and fork progression during development. We investigated the mechanisms by which binding of the origin recognition complex (ORC) and replication fork …
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Investigation of Cdc45 function during helicase activation
Eukaryotic DNA replication requires the stepwise loading of Mcm2-7 helicase complexes at origins of replication. These loaded helicases need to be activated during DNA replication initiation to unwind the DNA double helix and provide a ssDNA template for replicative DNA polymerases. Eukaryotic …
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Identifying a role for Ciz1 in the DNA damage response
In proliferating cells, cell cycle arrest and DNA repair are induced upon detection of DNA damage. The DNA damage response (DDR) to double strand DNA breaks is mediated by phosphatidylinositol 3-kinase-like protein kinases (PIKKs), which propagate DDR signalling by phosphorylating SQ/TQ motifs in …
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Origins of non-coding Y RNA family and its role in DNA replication
… RNAs that are required for chromosomal DNA replication initiation in vertebrates. Specifically, Y RNAs play an essential role in the establishment of new DNA replication forks during G1 to S phase transition. However, the mechanism by which Y RNAs contribute to initiation of DNA …
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The genome-wide analysis of the specification and activation of human chromosomal DNA replication origin sites
Background: Human DNA replication is a critical cellular process that initiates at tens of thousands of DNA replication origins. Next-generation sequencing (NGS) enabled the elucidation of some characteristics that define human replication origins. Initiation-site sequencing (iniSeq) utilises the …
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Does inappropriate DNA replication provide a mechanism for the de novo acquisition of drug resistance?
… investigates the ability of DTP cells to undergo DNA replication across multiple in vitro NSCLC DTP model systems. Interestingly, all DTP cells were able to undergo DNA replication rather than a specific subpopulation, and a large proportion of DTP cells are undergoing DNA replication at a given …
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Single-molecule studies of the mechanism of eukaryotic helicase activation
Eukaryotic DNA replication is a fundamental process that must occur accurately and only once per cell cycle. To ensure that origins only initiate once per cell cycle, the events of DNA replication initiation are temporally separated to different phases of the cell cycle. This regulation separates …
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Mechanism of Mcm10 function during DNA replication
… do so with limited errors. In eukaryotic cells, DNA replication is accomplished by a multi-stage process involving numerous protein assemblies. The core component of this process is the replicative DNA helicase, Mcm2-7. Mcm2-7 complexes are loaded around origins of DNA replication during G1 …
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Regulation of DNA replication by PP2A family phosphatases
In eukaryotes, DNA is replicated once and only once per cell cycle from hundreds of replication initiation sites (origins) across the genome. This process is strictly controlled as mis-regulation can cause replication stress and genome instability, characteristics of cancer. S phase …
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Regulation of cell fate asymmetry in Caulobacter crescentus by a complex of two component signaling proteins
… in predivisional cells. CtrA controls DNA replication, polar morphogenesis and cell division, and its cell-type and cell cycle-specific regulation is essential to the life cycle of Caulobacter. In swarmer cells, activated CtrA binds to the origin of replication and holds cells in G1. In …
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Molecular Characterization of Mth203 protein
… available concerning eukaryotic and bacterial replication, less is known about the molecular basis of replication initiation in archaea. In general, the archaeal proteins are simplified versions of their eukaryotic counterparts. Therefore, they are potentially simple model systems to understand …
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Molecular characterization of Mth203 protein
… available concerning eukaryotic and bacterial replication, less is known about the molecular basis of replication initiation in archaea. In general, the archaeal proteins are simplified versions of their eukaryotic counterparts. Therefore, they are potentially simple model systems to understand …
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Single-molecule studies of eukaryotic helicase loading
… of their progeny. The first step in eukaryotic DNA replication initiation is the loading of the heterohexameric Mcm2-7 helicase. In eukaryotes helicase loading is tightly regulated to ensure that this event only occurs during the GI phase of the cell cycle. Because helicase activation can only …
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Functional dissection of the S. cerevisiae helicase activating factor Cdc45
Eukaryotic DNA replication initiation requires two essential steps: helicase loading and activation. During the G1 to S transition, loaded Mcm2-7 helicases are activated by the S-CDK (S phase cyclin-dependent kinase)- and DDK (Dbf4-dependent kinase)- dependent recruitment of the helicase activating …
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Role of ORCA/LRWD1 in the maintenance of telomere integrity
… integrity is maintained by the coordination of replication, heterochromatin, and DNA damage repair machinery. In this thesis, I describe how Origin Recognition Complex-associated (ORCA/LRWD1), regulates telomere integrity in Alternative Lengthening of Telomeres (ALT) -positive cells. In the …
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Genome-wide Footprinting Uncovers Epigenetic Regulatory Paradigms by Revealing the Chromatin Occupancy Landscape
… and plasticity to modify transcription and replication programs, yielding a myriad of differentiated cell types and survival mechanisms to adverse environmental conditions. As these genomic processes require precise localization of DNA-binding factors, their dynamic temporal and spatial …
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Biochemical and Biophysical Characterization of Replicative Helicase Loading by <i>Vibrio cholerae</i> DciA, Crystal Structure of a Monoclonal Antibody Bound to a C-Terminal Fragment of EBNA-1 from Epstein Barr Virus
<p>DNA replication initiation is mediated by the binding of an initiator protein to genomic origins of replication to begin the process of loading replicative helicases onto the genome. The loading of the helicase requires the action of an accessory protein, named a “loader”. Studies on …
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