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Showing 1 to 13 of 13 for “"DNA purification"”.
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DNA Purification Process Optimization at Life Technologies Corporation
<p>This project focused on optimizing the plasmid DNA purification process at Life Technologies. These plasmids are designed to code for specialized proteins used by research universities, national laboratories, or research companies. Once cultivated and harvested, the plasmids must be analyzed for …
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A Novel Forensic DNA Profiling Method Based On Molecular Beacons Without Dna Purification
… along with the ever-increasing reliance on DNA to solve crimes, has led to a large DNA sample backlog, with violent crime turnaround taking an average of 103 days. The time and resource investment required for STR analysis is significant, and not all samples generate useful profiles. </p> …
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Identification of endophytic bacterial communities associated with South African crops: sorghum bicolor (L. Moench), pennisetum glaucum and arachis villosulicarpa
… study was to investigate the effect of different DNA extraction protocols on mDNA yield and quality, as well as the diversity of endophytic bacteria retrieved from root and stem tissues (0.1g or 0.3g) of sorghum, pearl millet and groundnut. Protocols used include two classical methods (CTAB- and …
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Conjugation of oligonucleotides for drug delivery via a linker derived from Meldrum’s acid and A capture-release method to synthesize long oligonucleotides
… describes a new catch and release method for DNA purification. This method has potential to simplify the isolation of full-length long oligonucleotides. For this project we aimed for the synthesis of a propargylated glycerol phosphoramidite as a 5’-end label. Incorporation of this alkyne into …
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Digital amplification for pathogen detection in bloodstream infections from whole blood
… enrichment through blood culture prior to DNA extraction. Nonetheless, state of the art diagnostic tests that detect pathogens directly from blood are available with limitations. Increased amount of sample processing and purification is required before detection of pathogens and turnaround …
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Historical Genetic Analysis Of Horned Lark (Eremophila Alpestris) Specimens From The Prairie Pothole Region
… and identified three distinct mitochondrial DNA clades. This study aims to genetically characterize Horned Larks from the North Dakota region using the mtDNA NADH dehydrogenase subunit 2 (ND2) gene. Horned Larks were sampled from museum collections, with ages ranging from 13 - 120 years old. …
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Emulsion templated assembly of composite particles
… to offer a sustainable alternative to commercial DNA purification kits for resource constrained labs. Magnetic photocatalysts (TiO2/Fe3O4/SiO2) were prepared for use as an easily recoverable system for adsorption and light-induced degradation of synthetic dyes (e.g. Rhodamine B and Methylene blue) …
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Genetics and Ecology of the Invasive Marine Alga, Sargassium Miuticum within Wales: A Multidisciplinary Approach to a Management Problem
… for this non-native alga. Based on the DNA sequencing markers investigated, both the invasive and native populations of S. muticum display low variability. Analysis of the TrnW_I mitochondrial intergenic spacer regions revealed the occurrence of a previously unrecorded haplotype from the …
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Accelerated Sepsis Diagnosis by Seamless Integration of Nucleic Acid Purification and Detection
… to expedite and automate the ``nucleic acid purification - qPCR sequence detection'' workflow.</p><p><bold>Methods and Results</bold> This task is evaluated to be best approached by combining immiscible phase filtration (IPF) and digital microfluidic droplet actuation (DM) on a fluidic …
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Development of a rapid molecular method for detection of C. perfringens and other pathogens in water sources
… as a tool to detect deoxyribonucleic acid (DNA) from two waterborne pathogens, i.e., Clostridium perfringens (C. perfringens) and Legionella pneumophila (L.pneumophila). In-house designed primers targeting the C. perfringens phospholipase C gene (plc) and previously published primers to …
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Nucleic acid sample preparation using magnetic ionic liquids as cell lysis and DNA extraction solvents
… Cell lysis is generally the first step in DNA sample preparation where the cell membrane is disrupted to release intracellular components such as DNA. Moreover, the isolation and enrichment of nucleic acids is essential to prevent false negative results from enzymatic detection methods that …
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Development and application of a PCR multiplex to assess the quality and quantity of forensic DNA extracts
Isolation of DNA from skeletonised human remains can be problematic. In addition to DNA degradation, enhanced by high temperature and humidity, there are often potent polymerase chain reaction (PCR) inhibitors present within the samples. It is therefore important to extract the maximum amount of …