Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 48 for “"DNA profiling"”.
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Marketised forensic DNA-profiling in England & Wales
… had a marked impact upon the field of forensic DNA analysis, and may affect the way in which expert DNA evidence is constructed. This empirical research project seeks to assess the impact that these public policy, and organizational, developments, have had on the perspectives of forensic …
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Assessing the effects of subpopulations on the application of forensic DNA profiling.
… frequencies based on a standard SGM Plus® DNA profile. These estimations will typically include correction factors to take into account issues such as substructuring of populations and sampling inefficiencies. It has been shown previously that regional genetic variation within the UK …
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A Novel Forensic DNA Profiling Method Based On Molecular Beacons Without Dna Purification
… Currently, the accepted method of analysis for profiling is Short Tandem Repeat (STR) profiling. This is a lengthy process, typically taking up to 3 days. The time necessary to generate an STR profile, along with the ever-increasing reliance on DNA to solve crimes, has led to a large DNA sample …
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Evaluating the use of capillary electrophoresis in the forensic DNA profiling of burnt teeth
… temperatures; making them a possible source of DNA from burnt human remains. DNA profiling is the current gold standard in forensic human identification, however, limited literature pertaining to DNA profiling of burnt teeth exists. The aim of this study was thus to evaluate the success of …
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Evaluation of forensic DNA profiling success on teeth that have been submerged in the ocean
… identification difficult. In these instances, DNA analysis becomes an invaluable tool for identification. Nonetheless, there is a notable gap in existing literature regarding the use of DNA from teeth for identifying human remains recovered from marine environments. This study therefore aimed …
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Evaluation of direct PCR for forensic DNA profiling and the development of a direct PCR multiplex
… of sample was explored in this study. Genomic DNA preparations at various concentrations and buccal cell counts were deposited on commonly encountered substrates, recovered and amplified using direct PCR before subjecting them to capillary electrophoresis. The electropherograms obtained were …
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The application of DNA profiling to the identification of victims in the Gulf war (1990-1991)
… need to improve the methodology employed in the DNA profiling of the Kuwaiti victims of the First Gulf War (1990-1991). The main challenges have involved developing the methodology in an attempt to increase the DNA recovery from the skeletal remains and also assess the preservation of DNA in the …
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A Comprehensive Evaluation of a new Direct Amplification System (PowerPlex® 18D) in Forensic DNA Profiling
… tandem repeat typing is the primary method of DNA identification used in the field of forensic science. Over the past several years the need to improve on this method has moved to the forefront of research. Due to the increasing number of criminal cases and the substantial backlogs most …
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Evaluating the role and impact of forensic DNA profiling on key areas of the criminal justice system
The advent of the modem technique of forensic DNA profiling has resulted in a lively union between one of the more advanced and dynamic disciplines of modern science and what is, arguably, society's most revered, influential and complex institution, the criminal justice system (CJS). The alliance, …
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DNA profiling of single sperm cells and single skin flakes in forensics using micromanipulation and whole genome amplification.
In forensic DNA profiling, the occurrence of complex mixed profiles is currently a common issue. Cases involving intimate swabs or skin flake tape liftings are prone to mixed profiles, because of more than one donor contributing to a DNA sample. By DNA profiling of single spermatozoa and skin …
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Purification of human dna for dna profiling from body fluid contaminated soil using superparamagnetic iron oxide nanoparticles (spions).
Extraction of good quality DNA from soil samples is difficult due to the co-extraction of humic acid and phenolic compounds which can inhibit downstream DNA profiling of samples. Several methods are available for DNA extraction from soil including a number of commercially available kits. We used …
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Dna Profiling of Captive Roseate Spoonbill (Ajaia Ajaja) Populations As a Mechanism of Determining Lineage in Colonial Nesting Birds.
Roseate spoonbills are colonial nesting birds with breeding grounds extending from the United States Gulf coast to the pampas of Argentina. The U.S. population suffered a severe bottleneck from 1890 to 1920. The population's recovery was slow and partially credited to migrations from Mexican …
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Are human identification methods effectively utilised? a retrospective review of unidentified remains between 2019-2020 at Salt River mortuary, Cape Town, South Africa
… methods exist ranging from visual recognition to DNA profiling. While DNA is utilised at Salt River Mortuary (SRM), there is slow turnaround and seldom feedback from the state laboratory. Thus in 2020, SRM began submitting samples for DNA profiling to a private laboratory (Unistel) as well. It is …
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Investigating the effect of NucleoSpin® Forensic Filters on DNA recovery from swabs
… method for human identification is forensic DNA profiling. Since trace evidence is often retrieved in small amounts, the optimal recovery of DNA from these samples is crucial. Methods for the recovery of touch DNA from swabs typically make use of a spin basket or filter, combined with a …
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Human provenancing - combined isotopic and genetic profiling of limited bone and teeth material of ancient human remains
… archaeology and anthropology. To date, DNA profiling is still the most effective approach for human identification. Despite the success story of DNA profiling, there is a constant search for additional methods to aid in the identification of human remains. In recent years increasingly …
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Investigation into DNA recovered from human teeth for forensic applications
… compromised, hard tissues may provide the only DNA source for identification. The QIAamp® DNA Investigator Kit is used in forensic laboratories worldwide, including in South Africa, to extract DNA for identification purposes. However, in local forensic casework, the DNA recovered from teeth is …
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Sex estimation of unidentified human remains: Concordance between morphological anthropological assessment and DNA analysis
… but were biologically sexed male based on DNA analyses. This study aimed to assess the agreement of sexing methods in a Western Cape South African forensic sample of skeletonised individuals (n=126), who were morphologically estimated to have biological sex as female (n=41). Of the 41 …
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Forensic human identification: Generating Y-STR data for the South African population
… ~9% of cases remained unidentified each year. DNA analysis was performed in 23.5% of cases. At the time of this study, unidentified bodies were in storage for up to two years, pending pauper burial. DNA profiling assists forensic human identification, and the analysis of markers on the …
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Efficiency of Using Commercial DNA Extraction Kits and the Organic Extraction Method in Removal of Polymerase Chain Reaction Inhibitors
… determination of a perpetrator of a crime using DNA profiling is obtaining good quality DNA. The substrate on which the body fluid is located may contain substances that can co-extract with DNA and may inhibit subsequent PCR analysis. In this study, the efficiency of the removal of such …
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Prediction of age from DNA
Currently DNA profiling methods only compare a suspect’s DNA with DNA left at the crime scene. When there is no suspect, it would be useful for the police to be able to predict what the person of interest looks like by analysing the DNA left behind in a crime scene. Determination of the age of the …
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