Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 25 for “"DNA probe"”.
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Construction and Use of Species-Specific Dna Probes for the Detection, Identification, and Enumeration of Human Colonic Bacteroides Species (Anaerobes, Hybridization, Microecology, Feces)
… which exploits differences in chromosomal DNA sequences among Bacteroides species. Random fragments of chromosomal DNA that are unique to each of 8 major colonic Bacteroides species were obtained and used to construct species-specific DNA probes. A procedure was devised whereby Bacteroides …
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Investigating Poloidal Bias in DNA Minicircles and Overhang influence on DNA Dehybridization
DNA is a dynamic molecule that undergoes constant physical manipulation by proteins. For instance, it experiences extreme bending when packaged into nucleosomes and undergoes strand separation (dehybridization) during transcription. Single-molecule assays offer a powerful approach to uncover the …
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Innovative molecular methods for fish species differentiation
… fish species have been developed. A DNA microarray was established using a set of 100 universal oligonucleotide probes. DNA probe signal patterns were observed to be speciesspecific, enabling species identification of unknown fish samples by comparing the generated DNA probe signal …
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Tuberculous pleural effusions : a prospective study of rapid diagnostic tests (adenosine deaminase, antigen capture enzyme-linked immunosorbent assay, and the polymerase chain reaction) and evaluation of a radiometric mycobacterial culture system
… immunosorbent assay (ELISA), and a specific DNA probe after amplification with the polymerase chain reaction. ADA was found to have a sensitivity of 0.77 and a specificity of 0.83 in the 109 patients tested, and values were significantly higher in tuberculosis patients compared with the other …
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DNA Biosensors Based on Gold-Coated Magnetic Nanoparticles
The interaction between DNA-functionalized surfaces/nanoparticles and free oligonucleotides in solution is the foundation of many devices such as DNA gene chips, therapeutic devices, and DNA biosensors. Electrochemical transduction provides a convenient means for studying DNA hybridization due to …
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DNA methylation detection using an engineered methyl-CpG-binding protein
DNA methylation, specifically the methylation of cytosine bases, is an important biomarker, as abnormal DNA methylation patterns are found in many different types of cancer. Currently, a small number of cancer hospitals evaluate the methylation status of the MGMT gene promoter to determine the best …
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Relationship of plasmids in Mycobacterium avium, Mycobacterium intracellulare, and Mycobacterium scrofulaceum
… avium isolate</i>, MDl, was cloned and used a a DNA probe to examine the relationship of MAIS plasmids. The pVT2 probe hybridized with plasmids isolated from MAIS strains from the environment, from patients without AIDS with pulmonary infections, and from AIDS patients with disseminated MAIS …
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Clinicopathological and molecular characterization of gastroesophageal junction (GEJ) adenocarcinoma before age of 40 years
… fluorescence in situ hybridization (FISH) DNA probe set specific for the locus specific regions 9p21 (p16), 20q13.2 and Y chromosome. We evaluated 663 patients with GEJ adenocarcinoma and further divided them into 2 age-groups of <or= 40 and >or= 50 years, rispectively. FISH with selected …
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Species-specific DNA probes for the identification of Actinobacillus actinomycetemcomitans
A DNA probe was developed for the identification of the periodontal pathogen, Actinobacillus actinomycetemcomitans. Chromosomal DNA was extracted from A. actinomycetemcomitans, digested with a restriction enzyme, Sau3A, ligated to plasmid DNA (pUC18) and transformed into JM109 cells to give a …
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Novel DNA probes for sensitive DNA detection
The ability to detect and interrogate DNA sequences allows further understanding and<br/>diagnosis of genetic disease. The ability to perform such analysis of genetic material<br/>requires highly selective and reliable technologies. Furthermore techniques which can use<br/>simple and cheap …
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Characterization of inter-genomic and telomeric G-quadruplex structures through atomic force microscopy
The G-quadruplex DNA structure, a secondary folding motif of DNA, is found throughout the genome. The dynamics of these structures has been seen to affect processes such as telomerase elongation and regulation of gene transcription. Typical investigation techniques require these structures to be …
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Molecular Study of the B19 (Human) Pathogenic Parvovirus
… (human) parvovirus is a small single stranded DNA virus of 5.4 kilobases. B19 is specific for erythroid progenitor cells and has been propagated in vitro only with human erythroid bone marrow. Replication of viral DNA and the viral protein products of B19 appear similar to those of other animal …
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DNA hybridization : fundamental studies and applications in directed assembly
Programmed self-assembly using non-covalent DNA-DNA interactions is a promising technique for the creation of next-generation functional devices for electronic, optical, and magnetic applications. This thesis develops the ability to tailor surfaces for the DNA-driven assembly of molecular, nano-, …
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Optimization of Molecular Beacon-Based Multicomponent Probes for Analysis of Nucleic Acids
… of single nucleotide substitutions (SNS) in DNA and RNA has a growing importance in biology and medicine. One traditional approach for recognition of SNS takes advantage of hybridization probes that bind target nucleic acids followed by measuring ?Tm, the difference in melting temperatures of …
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Detection of Babesia species in domestic and wild Southern African felids by means of DNA probes
… and differentiate between blood parasites using DNA probes. The RLB consists of three basic steps, the first being amplification of the variable region (V4) in the 18S rRNA gene using genus-specific primers where one is labelled with biotin. This is followed by a blotting step, where the …
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Genetic and biochemical interactions between the DNA replication initiator and a chromosome architecture protein in Bacillus subtilis
… and epistatic relationship between the DNA replication initiator DnaA and the nucleoid-associated protein Rok in Bacillus subtilis. Using ChIP-seq, I characterized the genome-wide association of DnaA and its regulator YabA. I found that DnaA and YabA associated with over 30 chromosomal …
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Understanding the functions of HMGB proteins in the mechanism of action of cisplatin
High mobility group box (HMGB) proteins are DNA-binding proteins that regulate many important DNA-related processes. They are known to recognize the major lesion present in cisplatin-modified DNA, and have been assumed to increase cisplatin cytotoxicity by "shielding" the damaged site from the …
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Dynamics of DNA Breathing and Folding for Molecular Recognition and Computation
… development of the molecular beacon, as a new DNA probe for DNA genotyping, DNA computation and biophysical studies of DNA conformations. Molecular beacons are single-stranded DNA molecules that form a stem-and-loop structure. A fluorophore and a quencher are grafted at their two ends to report …
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Genetic, environmental, and physiological factors involved in the precocious sexual maturation of chinook salmon (Oncorhynchus tshawytscha)
… and non-accelerated groups respectively. DNA fingerprinting was used to detect differences in allele distribution between precocious males and randomly selected fish, such differences indicate genetic involvement. Two oligonucleotide DNA fingerprinting probes were developed, however the …
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Towards smart molecular biosensors: Modulation of DNA strand displacement reactions with transcription factors for robust and tunable arsenic quantification
… in synthetic biology tools, such as isothermal DNA amplification, CRISPR-Cas and DNA strand displacement (DSD), have accelerated this transition and the ongoing COVID-19 pandemic has made biological testing ubiquitous. Nucleic acid diagnostics have been at the forefront of this trend, …
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