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Showing 1 to 20 of 138 for “"DNA polymerase"”.
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The Characterization of Mip1 DNA Polymerase
… are the only organelle to possess their own DNA outside of the nucleus. A single polymerase is primarily responsible for the replication and repair of mtDNA. In humans and other animals, this enzyme is known as polymerase gamma (Pol γ) and it is orthologous to Mitochondrial DNA Polymerase 1 …
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The Characterization of Mip1 DNA Polymerase
… are the only organelle to possess their own DNA outside of the nucleus. A single polymerase is primarily responsible for the replication and repair of mtDNA. In humans and other animals, this enzyme is known as polymerase gamma (Pol γ) and it is orthologous to Mitochondrial DNA Polymerase 1 …
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Mcm10 regulates the turnover of DNA polymerase alpha.
… Critical for Mcm10's role at the fork is its DNA binding activity. Utilizing the crystal structure of the conserved internal domain of X. laevis (x) Mcm10, we identified residues that, when mutated, significantly reduced xMcm10's ability to bind DNA in vitro. Importantly, the corresponding …
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Disruption of Dna Polymerase Zeta Promotes An Innate Immune Response
<p>DNA polymerase ζ (pol ζ) is a specialized polymerase that protects cells from the consequences of DNA damage and stress. Without pol ζ function mammalian cells and yeast are exquisitely sensitive to genotoxic stresses including ultraviolet irradiation and platinum compounds such as the …
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The molecular basis of nucleotide recognition for T7 DNA polymerase
DNA replication demands extraordinary specificity and efficiency of catalysis from a DNA polymerase. Previous studies on several DNA polymerases suggested that a rate-limiting conformational change preceding chemistry accounts for the high specificity following the induced fit mechanism. However, …
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DNA polymerase interactions and their role In translesion synthesis substitutions.
Translesion synthesis (TLS) is a DNA damage tolerance mechanism that allows DNA replication to continue after a high fidelity (HiFi) polymerase stalls at a lesion. Upon stalling, the HiFi polymerase must be substituted with a TLS polymerase. The reverse substitution must occur promptly after lesion …
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Expression and Functional Analysis of Hepatitis B Virus DNA Polymerase
PART I: Expression of chimeric DNA polymerase of human and duck hepatitis B virus Hepatitis B Virus (HBV) DNA polymerase is a multifunctional protein with four domains, terminal protein (TP), spacer, reverse transcriptase (RT), and RNaseH. Since HBV DNA polymerase is very unstable and rapidly …
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The Isolation and Partial Purification of DNA Polymerase-Beta from Soybean
… to those previously determined for animal polymerase-beta. Therefore, since the structure of polymerase-beta shows significant evolutionary conservation in both plant and animal species and given strong functional correlations among species, polymerase-beta performs a vital role in plants …
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Dna Polymerase Θ (Polq) and The Cellular Defense Against Dna Damage
<p>In mammalian cells, DNA polymerase θ (POLQ)<strong> </strong>is an unusual specialized DNA polymerase whose <em>in vivo</em> function is under active investigation. The protein is comprised of an N-terminal helicase-like domain, a C-terminal DNA polymerase domain, and a large central domain that …
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Studies on Mutants of the Dna Polymerase Gene of Bacteriophage-T4
Made available in DSpace on 2014-12-09T22:04:19Z (GMT). No. of bitstreams: 1 6915258.pdf: 2802099 bytes, checksum: 750fc3cf30b1195fca2df2e5b2c293ff (MD5) Previous issue date: 1969
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Analysis of DNA polymerase activities involved in bovine parvoviral DNA replication
The polymerase activities involved in bovine parvoviral (BPV) DNA replication in vivo and in vitro have been described. In the in vitro system, purified and partially purified enzymes were used and the replication products were analyzed by gel electrophoresis and restriction enzyme digestion. DNA …
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Unveiling Dna Polymerase Synthesis And Proofreading Activities - One Molecule At A Time
<p>DNA polymerases maintain the genome integrity from one generation to the next by faithfully synthesizing new DNA and by participating in DNA repair processes. The Klenow fragment of E. coli DNA polymerase I has served as a model polymerase for decades because it is straightforward to purify, …
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The effect of oxidized guanines on DNA synthesis by DNA polymerase β
DNA replication is the fundamental biological task of duplicating genetic information for passing on to a daughter cell. DNA polymerases are the molecular machines that read the parent DNA strand and instruct the addition of correct corresponding nucleotides (also termed fidelity) to form a new DNA …
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Structure-Guided Development of Antifungal Protein Farnesyltransferase Inhibitors and DNA Polymerase Engineering
… In the second study, an intein was inserted into DNA polymerases to produce temperature controlled enzymes. The intein controlled DNA polymerases only showed activities after intein splicing triggered by high temperature (>60oC), enabling the capability of conducting “hot-start” reactions by …
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Effect Of Dietary Folate Restriction On Colon Carcinogenesis In Dna Polymerase β Haploinsufficient Mice
… (WT) animals, as did a BER deficiency in DNA polymerase Β haploinsufficient (Β-pol+/-) mice exposed to 1, 2-dimethylhydrazine (DMH), a known colon and liver carcinogen. We combined both folate restriction and a BER deficiency to determine the fate of colon tissue after exposure to DMH. Of …
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A Rapid and Power-Efficient Laser-Induced Graphene Micro-Thermocycler for DNA Polymerase Chain Reaction
By amplifying millions or billions of copies of a DNA’s specific segment, polymerase chain reaction (PCR) has a wide range of applications in DNA cloning, forensics, medical, and infectious disease detection. As a result, the technology is becoming more and more widespread as the COVID-19 pandemic …
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DNA polymerase beta inhibitor pamoic acid : toxicity to metakaryotic human cancer stem cells (HT-29)
… and tumor growth, use a peculiar mode of DNA synthesis and segregation that involves inter alia expression of large amounts of RNA polymerase beta during DNA synthesis. It was hypothesized that an inhibitor of DNA polymerase beta would be toxic to metakaryotic stem cells at concentrations …
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PFU DNA polymerase--a study of amplification error rate and subsequent impliations for high fidelity mutational spectrometry
Thesis (M.S.)--Massachusetts Institute of Technology, Dept. of Civil and Environmental Engineering, 1994.
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