Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"DNA packaging"”.
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Structure and Function Study of Phi29 DNA packaging motor
… powerful nanomotor is employed by the tailed dsDNA virus to package the genome into a preformed protein shell during the process of replication. The bacteriophage phi29 is an excellent model for investigating the viral DNA packaging mechanism. The phi29 DNA packaging motor is composed of three …
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Relating the structure of the HSV-1 UL25 DNA packaging protein to its function
… is a minor capsid protein that is essential for packaging the full-length viral genome into preformed precursor capsid. It is also important in virus entry and recently has been implicated in the egress of the virus from the cell (Coller et al., 2007, Preston et al., 2008). The crystallographic …
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The role of the herpes simplex virus type 1 UL33 protein in DNA packaging
… essential for the cleavage of concatemeric viral DNA into monomeric genomes and their packaging into preformed capsids. Several lines of evidence have suggested that UL33, along with the UL15 and UL28 gene products, forms part of a terminase enzyme responsible for catalysing this process. This …
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TOWARDS AN UNDERSTANDING OF GENETIC SELECTION IN BREAST CANCER
… 812 stable contiguous genomic regions. Stable DNA targets were confirmed by quantitative PCR in six breast cancer cell lines. Stable regions contained 9,463 protein coding genes, of which expression of 7,489 were analyzed. We found 78% (5804/7489) of genes had both stable copy number and …
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Identification and Molecular Analysis of Dna In Exosomes
… functional cargo components, such as protein, DNA, and RNA. While protein and RNA exosome content has been extensively studied, very little work has been done to characterize exosomal DNA. Here, we demonstrate that exosomal DNA is heterogeneous and its packaging into exosomes is dependent on …
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CRAMP1 drives linker histone expression to enable Polycomb repression
… of the core histones (H2A, H2B, H3 and H4) in DNA packaging has been well-understood for decades, but the function of the linker histone (H1) remains enigmatic. H1 compacts nucleosome arrays in vitro, but the multiple H1 subtypes and variants encoded in mammalian genomes (11 in mice and humans) …
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Improved Dynamic Range, Quantitation, and Characterization of Histone H4 Post-Translational Modifications: A Top Down Mass Spectrometric Approach
"Intimately associated with DNA, histone proteins serve as both a structural scaffold for DNA packaging into the nucleus and an epigenetic means for the regulation of gene expression. One such histone-based mechanism for transcriptional regulation is post-translational modification (PTM) of …
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The Molecular Mechanism of Platelet Activation in Inflammation
Histones, typically confined to the nucleus for DNA packaging, exhibit a critical shift in function when released extracellularly, acting as damage-associated molecular patterns (DAMPs) that contribute to inflammation and thrombosis. This study investigates the role of extracellular histones in …
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Maximum Entropy Optimization: a General Approach to Study Ordered and Disordered Proteins Reveals Key Features of Protein Phase Separation
… 5, we combine MOFF with a chemically accurate DNA model to study the phase behavior of chromatin regulators that are crucial for heterochromatin organization and their interactions with DNA. Notably, a layered organization was observed in condensates formed by mixing HP1, histone H1, and DNA. …
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Effect of Naturally Occurring DNA Modifications on DNA Structure and Packaging
In eukaryotes, the genomic double-stranded DNA (dsDNA) coils around histones to form nucleosomes. Arrays of these nucleosomes bundle together to generate chromatin. Most DNA-related processes require interactions between chromatin-protected DNA and cellular machinery. Access of cell machinery to …
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Rate zonal density gradient ultracentrifugation analysis of repair of radiation damage to the folded chromosome of Escherichia coli
… of Escherichia coli and of unfolded chromosomal DNA was investigated by sedimentation on neutral sucrose gradients after irradiation with 60Co gamma-rays and ultraviolet light (2S4nm). Irradiation both in vivo and in vitro was used as a molecular probe of the constraints on DNA~packaging in the …
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Chromosomes and spermatozoa of the great apes and man
… to the content of highly repeated satellite DNAs in man and homologous sequences in the great apes. This has shown that the banding patterns are not informative about these sequences, and that they must reflect a degree of chromosome organization due to DNA packaging rather than DNA …
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Role of the Small Terminase Subunit Encoded by Staphylococcus Aureus Pathogenicity Island SaPI1 in Formation of SaPI1 Transducing Particles
… suggests that it plays a role in SaPI1-specific packaging into transducing particles by complexing with the 80α large terminase subunit and redirecting recognition to a pac site on SaPI1 DNA from 80α DNA. The effects of deleting the small terminase genes in SaPI1 and in a prophage copy of 80α are …
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Analysis, Optimization and Application of AAV Capsid Assembly using Escherichia coli
… delivery was also studied and strategies for DNA encapsidation were tested. Among 13 known human and nonhuman primate AAV serotypes, AAV serotype 2 (AAV2) is the most studied and AAV serotype 5 (AAV5) is the most genetically divergent serotype. To analyze AAV capsid assembly, AAV2 and AAV5 VP3 …
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Mechanisms and regulation of dsDNA break repair in the Sulfolobus genus of thermophilic archaea
DNA is constantly subjected to chemical and mechanical damage. The ability to repair the lesions sustained is essential for all life. Double stranded DNA (dsDNA) breaks are especially toxic as both antiparallel strands of DNA are severed. The most high fidelity mechanism available to repair this …