Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 19 of 19 for “"DNA modifications"”.
-
Covalent DNA Modifications in Phage and Bacterial Dynamics
… enzymes and CRISPR systems, and the covalent DNA modifications that neutralize them. Multiple targeted nucleases equip bacteria with an innate immune response against phage, and CRISPR systems provide an adaptive immune response. I report three main studies. 1) To study the human gut …
-
Dynamic DNA Nanotechnology for Probing Single Nucleotide Variants and DNA Modifications
In the last decades, various DNA hybridization probes have been developed that attempt to conquer the challenge of single-nucleotide-variants (SNVs) detection. Even though a powerful toolbox including the toehold-exchange reaction, the dynamic ‘sink’ design, and the polymerase chain reaction (PCR) …
-
Effect of Naturally Occurring DNA Modifications on DNA Structure and Packaging
In eukaryotes, the genomic double-stranded DNA (dsDNA) coils around histones to form nucleosomes. Arrays of these nucleosomes bundle together to generate chromatin. Most DNA-related processes require interactions between chromatin-protected DNA and cellular machinery. Access of cell machinery to …
-
The Placenta Methylome: Novel Insights into DNA Modifications in the Human Placenta
The DNA methylation profile of the placenta is unique as compared to other somatic tissues and is marked by global hypomethylation. The placental methylome has been shown to modulate developmental processes, intrauterine exposures, and placental function. However, the precise mechanisms underlying …
-
Statistical and computational approaches for the analysis of high-throughput epigenomic data /
Epigenetic mechanisms, such as DNA modifications, are of great importance in all living things. Despite the importance of DNA modifications, many of the difficulties involved in establishing and characterizing epigenetic profiles discourage researchers from following this path of research. TOP-seq …
-
Characterization of a novel family of nucleolus localized RNAs
… are composed of tandem copies of hundreds of rDNA repeats. However, the sequence beyond the rDNA array within the p-arms of NOR containing acrocentric chromosomes is largely unknown. In human cells, ~400 copies of rDNA repeats code for rRNAs. Interestingly, at any time point only ~50% of rRNA …
-
Characterising the role of TET2 in oestrogen receptor positive breast cancer
… are thought to be capable of binding methylated DNA, whilst TET2 is also implicated in DNA methylation pathways through its iterative conversion of methylated DNA to several additional DNA modifications, 5-hydroxymethylcytosine, 5-formylcytosine, and 5-carboxylcytosine. These results implied a …
-
Developing Novel Methods to Identify RNA-Associated Mechanisms for Inheritance
… was previously thought to be limited to DNA modifications and DNA binding proteins. But recent studies have expanded our understanding of inheritance to include RNA and RNA binding proteins. We currently lack methods to identify and enrich for RNA binding proteins that might be involved …
-
Studies on the detection of nucleotides and oligonucleotides by mass spectrometry
… and modified nucleotides such as DNA adducts by mass spectrometry. DNA adducts are important because they are formed during chemical carcinogenesis as well as during anti-cancer chemotherapy. However, DNA adducts are not routinely monitored due to difficulties associated with their …
-
Exploiting Lentiviral Integrations to Profile Chromatin
Current methods for profiling histone modifications and protein localisation along chromatin suffer from critical limitations: the gold standard, ChIP-seq, relies on high-quality antibodies, while other antibody-free techniques like DamID are limited by the availability of specific DNA sequences. …
-
A photochemical method for the detection of cytosine modifications in DNA
Covalent modifications to DNA bases represent an important form of epigenetic information which contributes to the regulation of gene expression in many cellular processes throughout development and differentiation. Aberrant modification patterns can also signal or drive the emergence of genetic …
-
Recombination and Excision: DNA Repair Proteins in Prokaryotic Host-Virus Conflicts
… My thesis investigates how mechanisms of DNA repair, specifically recombination and base excision, have been co-opted by phages and bacteria to execute non-canonical immune and counter-immune functions in prokaryotic host-virus genetic conflicts. CRISPR-Cas adaptive immune systems, found …
-
Design and Applications of Split-Luciferase Systems in Vitro and in Cellulo
… system as applied to the specific detection of DNA, DNA modifications, and small molecule kinase inhibitors both in vitro and in cellulo. We have previously developed one of the first direct methods to detect any double stranded DNA sequence by attaching DNA binding domains, specifically …
-
Fundamentals of Excess Electron Transport in Biologically Relevant Systems
… lesions that form via ultraviolet irradiation of DNA. While the distribution of thymine dimers is non-random, it is also not yet predictable. Thymine dimer accumulation is likely to be controlled by both its formation and reversion. Formation of thymine dimers occurs via direct excitation of …
-
Regulation of nucleosome dynamics
… called nucleosomes, where 147 base pairs of DNA are wrapped around a protein core. Stable packing of DNA in nucleosomes imposes a barrier for accessibility of genetic code on DNA for replication, transcription, and repair. The dynamics of nucleosomal DNA provide a mean for gene regulation by …
-
Programming of cardiovascular disease: an exploration of epigenetic mechanisms
… involvement of more recently identified cytosine modifications; 5-hydroxymethylcytosine (5hmC), 5- formylcytosine (5fC) and 5-carboxylcytosine (5caC), has not been characterised during germ cell ontogeny. Using immunofluorescence to study DNA modifications during late gestation I identified that …
-
Development Of Single-Cell Transcriptomic & Epigenomic Sequencing Technologies To Assess Cell-Type-Specific Gene Regulatory Programs In Mammalian Brains
… technology to assess the relationship of two DNA modifications with transcriptional outcomes in the same single cell. The current state-of-the-art DNA methylome profiling technologies rely solely on bisulfite conversion, which cannot distinguish 5hmC from 5mC. Because 5hmC is significantly …
-
DIABETES IMPAIRS THERAPEUTIC EFFECT OF ENDOTHELIAL PROGENITOR CELL EXOSOME-MEDIATED MYOCARDIAL REPAIR
… that epigenetic mechanisms, such as histone and DNA modifications for gene silencing, promote diabetes-induced vascular complication. Therefore, we hypothesized that diabetic EPCs produce exosomes of altered and dysfunctional content that compromise their reparative function in ischemic heart …
-
DEVELOPING CHEMICAL TOOLS TO MAP MOLECULAR MECHANISMS THAT DRIVE DISEASE
… by molecular level events. Within this context, DNA encodes for cellular instructions and proteins execute these genetic protocols to ensure the cell’s components are functioning properly. Thus, the underlying biochemistry within the cell is tightly regulated to ensure vitality. However, when …