Global ETD Search
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Showing 1 to 4 of 4 for “"DNA microinjection"”.
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Bovine embryo microinjection, culture, microsurgery, and DNA analysis by the polymerase chain reaction technique
… of cellular material from bovine morulae for DNA amplification by the polymerase chain reaction technique (PCR), and evaluated the use of DNA microinjection, in vitro culture, morula bisection, and PCR for production and selection of transgenic bovine preimplantation embryos. In vivo …
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Control of endometrial secretion in cattle and production of transgenic swine
… schemes. Pronuclear microinjection and zygote culture in excised mouse oviducts also was assessed. Synchronization/superovulation procedures were: 1) sows observed for estrous behavior (NAT), 2) cyclic gilts synchronized with altrenogest (ALT) for 15 to 19 d and …
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Gene injection in the bovine: effect of time of microinjection and nuclear transfer technologies
… in vitro. Experiment 1 examined the effect of DNA microinjection at 11, 15 and 19 h after fertilization (haf) on survival rate and DNA detection frequency by polymerase chain reaction (PCR). There was no difference in transgene detection frequency between treatments (53% at 11; 50% at 15; 48% …
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In-vitro developmental potential of bovine oocytes obtained by transvaginal follicular aspiration as related to their morphological quality and after microinjection of DNA
… follicular aspiration (TVFA) and following DNA injection was examined. Eight cows were subjected to twice weekly TVF A for 16 wk. Oocytes retrieved were graded and placed in an in-vitro maturation, fertilization and co-culture (IVMIIVFIIVC) program. Two thirds of oocytes were injected with …