Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 33 for “"DNA methyltransferases"”.
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DNA Methylation and DNA Methyltransferases in the Clonal Raider Ant, Ooceraea biroi
<p>DNA methylation and DNA methyltransferase enzymes (DNMTs) are ubiquitous and predate the origin of eukaryotes. In animals, DNA methylation primarily is carried out by DNMT1, which targets hemi – methylated DNA and maintains methylation patterns through cycles of cell replication, and DNMT3, the …
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DNA and Ligand Binding and Kinetic Properties of Wild-Type and Mutant RsrI [n6-Adenine] DNA Methyltransferases
… characterization of M•Rsr I in vitro, DNA binding analysis in vivo was performed using the bacteriophage P22-based challenge-phage assay. I isolated a catalytically impaired mutant, M•RsrI (L72P), that, unlike wild-type M•RsrI, binds in a methylation-sensitive, site-specific …
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Predicting protein-DNA interactions using statistical modeling and biophysical representations of high-throughput sequencing data
… or protein complexes, and their interaction with DNA by applying statistical modeling on high-throughput sequencing data. First, we investigate the action of DNA methyltransferases in a yeast species naturally free of DNA methylation. Using bisulfite-seq and RNA-seq, we measure DNA methylation …
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Mechanistic studies of Fusobacterium genetic and defense systems
… systems with a focus on the role of DNA methyltransferases and endonucleases in host defense and genetic recalcitrance in several strains of Fusobacterium through bioinformatic and biochemical approaches. Altogether, over 15 DNA methyltransferases were characterized. Most notably, we …
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Studies of a DNA modification methyltransferase from Rhodobacter sphaeroides
The DNA methyltransferases represent an interesting class of enzymes for the study of protein-DNA interactions due to their high specificity, structural simplicity, and biological importance. RsrI methyltransferase (M$\cdot$RsrI) from Rhodobacter sphaeroides recognizes duplex d(GAATTC) and deposits …
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XRCC1 & DNA MTases : direct and indirect modulation of inflammation-induced DNA damage
… exposure to RONs. We further hypothesize that DNA methyltransferases are responsible for the memory of genotoxic insult, and the epigenetic propagation of genomic instability, following exposure to genotoxins. Here, we exploited cell lines engineered to carry deficiencies in BER to study repair …
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Design and synthesis studies of new s-adenosyl-l-methionine analogues
… and methionine catalyzed by adenosyltransferase. Methyltransferases (MTs) transfer the activated methyl group from the sulfur center to a specific position in a variety of substrates, e.g., DNA, RNA, proteins, and secondary metabolites. Methyltransferases have been linked to various diseases, …
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The Role of DNA Methylation in Addiction and Depression
Despite abundant expression of DNA methyltransferases (Dnmt’s) in brain, the regulation and behavioral role of DNA methylation remain poorlyunderstood. I found that Dnmt3a expression is persistently regulated in nucleus accumbens (NAc),a key brain reward region, by chronic cocaine or chronic social …
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Synthetic approaches to the understanding of DNA nucleobase methylation
DNA methylation is a major source of genetic variation and cancer. Methylation occurs when nucleophilic DNA bases react with methylating agent methyl methanesulfonate (MMS), dimethyl sulfate (DMS), N-methyl-N-nitrosourea (MNU), N-methyl-N-nitro-N-nitrosoguanidine (MNNG), etc. …
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An investigation of the epigenetic and transcriptional changes which follow Epstein-Barr virus infection of germinal centre B cells
… B cells is followed by the up-regulation of the DNA methyltransferases, DNMT3A, and the down-regulation of DNMT1 and DNMT3B, a pattern of expression which is re-capitulated in HL cell lines. I have also shown that the major EBV oncogene, LMP1, is responsible for the down-regulation in GC B cells …
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Molecular Characterization of Mammalian De Novo DNA Methyltransferase Chromatin Recruitment
… diseases. The genomic localization of de novo DNA methyltransferases 3A (DNMT3A) and 3B (DNMT3B) is facilitated in part by chromatin "reader" domains to establish DNA methylation patterns genome-wide. Recent work has highlighted a role for the PWWP reader domain in directing recruitment of …
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Investigating Epigenetics as a Mechanism to Regulate Bacterial Heterologous Expression
Epigenetic DNA methylation in bacteria is involved in phage defense, and it can also affect gene transcription. Epigenetic methylation as part of restriction-modification systems is frequently considered a barrier in synthetic biology since it can prevent the introduction of heterologous genes to …
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Application of High-Performance Liquid-Chromatography Mass-Spectrometry Platform to Study Metabolism and Epigenetic Control of Metabolism
… of the information flow in biology, along with DNA, RNA, and proteins. Metabolomics is an analytical approach that seeks to comprehensively analyze naturally occurring small molecules and quantify their dynamic changes in biological systems. In recent years metabolomics has begun to provide …
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The role of Ten Eleven Translocation enzymes in the hair follicle mesenchyme
… complete reversal of hair loss. Tet2 promotes DNA demethylation by the hydroxylation of 5mC to 5hmC which in turn causes gene transcription activation. Dermal papilla (DP) cells located within the hair follicle are responsible for the regulation of development and the growth of hair follicles. …
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Contribution of Lsh to DNA methylation reprogramming in embryonic stem cell, epiblast stem cell and embryoid body model systems
DNA methylation is a key epigenetic mark which undergoes global reprogramming during early mammalian embryonic development, resulting in almost complete erasure of the mark after fertilisation of the zygote. Genome-wide patterns of DNA methylation are subsequently re-established in the implanting …
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Kupplung der DNA-Methyltransferase M.SssI mit Triplehelix-bildenden Oligodesoxynucleotiden
DNA modifying enzymes, like DNA methyltransferases (DNA MTases) and restriction endonucleases (REases), could in principle be used to map or manipulate genomes. However, these enzymes are inappropriate for that purpose because of their generally short recognition sequences. An increase of their …
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Regulation and activity of the 5-methylcytosine DNA glycosylase ROS1 contributes to DNA methylation patterning across development
DNA methylation patterning is a consequence of opposing activities of DNA methyltransferases and DNA demethylases. A 5-methylcytosine DNA glycosylase, ROS1, removes DNA methylation from the Arabidopsis genome. In flowering plants, two distinct female gametes, the egg cell and the central cell, are …
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Targeted Epigenetic Editing to Increase Adult Pancreatic Β-Cell Proliferation
… with age. I hypothesized that changes in DNA methylation contribute to the age-related decline in proliferative capacity in human β-cells, and that altering the DNA methylome in a targeted manner could improve proliferative capacity. To investigate this hypothesis, I sought to profile the …
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Investigating the impact of pharmacological exposures on the developing epigenome
… myeloid leukaemia. Following incorporation into DNA, both drugs have been demonstrated to induce depletion of DNA methyltransferases (DNMTs), removal of DNA methylation and induction of DNA damage. I show that treatment of mouse embryonic stem cells (mESCs) with 5-azacytidine and decitabine, …
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Methyl-CpG-Binding domain proteins and histone deacetylases in the stage-specific differentiation of olfactory receptor neurons
DNA methylation-dependent gene silencing, catalyzed by DNA methyltransferases (DNMTs) and mediated by methyl binding domain proteins (MBDs) and histone deacetylases (HDACs), is essential for mammalian development, with the nervous system demonstrating particular sensitivity to perturbations. Little …
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