Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"DNA metabolism"”.
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Functional Roles of Nucleases in DNA Metabolism and Genome Stability
… for genome stability. Both areas focus on DNA repair pathways that require the action of nucleases, specifically Exonuclease 5 and Ribonuclease H2. First, I describe the biochemical and molecular characterization of the novel Exonuclease 5 family of enzymes from S. cerevisiae, S. pombe, and …
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BIOLOGICAL CHARACTERIZATION OF TWO PUTATIVE DNA METABOLISM ENZYMES IN DEINOCOCCUS RADIODURANS
… protein that translocates double-stranded DNA during cell division. In archaea, herA is an essential gene that encodes an enzyme believed to be important for recombinational DNA repair. It is typically found in an operon with a gene that codes for a nuclease, nurA. Homologs of herA and nurA …
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Production of poly(adenosine diphosphate-ribose) polymerase-1: development of an efficient production and purification protocol
… in a range of activities associated with DNA metabolism, playing a key role in maintaining the integrity of DNA and chromatin structure. As such, this enzyme is likely to provide a useful target when using a rationale drug design approach to develop pharmaceutical reagents including cancer …
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Mechanisms of Cell Death Initiated in Herpes Simplex Virus Thymidine Kinase Expressing Colon Tumor Cells Treated with Ganciclovir and UCN-01
… by studying the effects on the cell cycle, DNA metabolism and damage signaling, and finally tested in the transgenic adenocarcinoma of mouse prostate (TRAMP) model. Colon tumor cells treated with GCV arrest in S-phase, and cells treated with the combination of UCN-01 and GCV also undergo …
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Using "Designer" Nucleosomes to Study Enzymatic Crosstalk Between Histone Ubiquitylation and Histone Methyltransferases
… for signal transduction affecting all types of DNA metabolism. Histone proteins in particular are extensively post-translationally modified (PTM), and many of these individual modifications have been studied in depth. I have been interested in how the complex repertoire of histone PTMs are …
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The molecular bases of tryptophan replacement effects in RecA: A biochemical and biophysical characterization of single- and null- tryptophan mutant Escherichia coli RecA proteins
Recombinational DNA repair is an essential component of DNA metabolism and the proteins required for recombinational repair pathways are highly conserved. The Escherichia coli RecA protein is studied as a prototypic strand exchange protein with structural and biochemical similarity to the archaeal …
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Subcellular localization and visualization of RecA and ImuAʹ in mycobacteria
… assault. The potential target: mycobacterial DNA metabolism. For this, an in-depth understanding of the mechanisms of DNA repair and mutagenesis in mycobacteria is required. RecA and ImuAʹ are DNA damageinducible proteins implicated in DNA damage repair and tolerance in Mtb. RecA is a key …
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Environmental proteome profiling applied to the study of polybacterial metal resistance and adaptation
… with proteins involved in protein and DNA metabolism being the most largely modulated. In the second case study, an unsequenced mixed culture was exposed to cadmium and had its dynamic response analyzed. While the community responded significantly to all shock durations, the greatest …
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Structure/function analyses indicate novel roles for mycobacterial DnaQ homologs in genome maintenance
Mycobacterial DNA metabolism is of increasing interest as both an underexplored source of new targets for anti-tuberculosis (TB) drug development and for its potential role in the emergence of drug-resistant Mycobacterium tuberculosis strains. However, the redundancy implied by the sizable …
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Genetic and biochemical analysis of nitrogen metabolism in ruminal anaerobic bacteria: a comparison between ruminococcus albus 8 and prevotella ruminicola 23
… Prevotella ruminicola 23 during growth on, and metabolism of, different nitrogen sources. Both R. albus 8 and P. ruminicola 23 were grown using different nitrogen sources; ammonia, urea, or peptides for R. albus 8 and ammonia or peptides for P. ruminicola 23 as the sole nitrogen source. Gene …
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Functions of Human DNA2 and its Protein Partners in DNA End Resection
Living organisms must keep their DNA content intact to assure the integrity of their genomic information. DNA replication and DNA damage repair are the main efforts to accomplish this task. Hence, the failure of these mechanisms could lead to genomic instability and subsequently development of …
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Nuclear export signals (NESs) in Arabidopsis thaliana : development and experimental validation of a prediction tool
… activity include 9 transcription factors and 2 DNA metabolism-related proteins. Furthermore, it was established that the amino acid residues located between the hydrophobic residues in the NES as well as the protein structure of the regions around the NES could modify the nuclear export activity …
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Developing high-resolution methods to study DNA and RNA dynamics
Nucleic acids, including DNA and RNA, are one of the most important biomacromolecules inside the cell. DNA stores genetic information, while RNA has more versatile roles including conveying and deciphering genetic information, catalyzing biological reactions, conducting post- transcriptional …
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Identification of a gene that makes-plants-gigantic-1: characterization of mpg1, a novel mutant of rice
… increase plant biomass by engineering a transfer DNA (T-DNA) expression cassette that drives the overexpression of sucrose transport in rice phloem. The hypothesis simply suggested transporting more carbon (sucrose) from leaf tissue to heterotrophic tissues in the plant would increase the biomass …
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Characterization of an agrobacterial plasmid inducible for transfer by mannopine and evolution of the core replication and transfer functions of repabc plasmids with class i quorum-sensing and transfer systems
… reasons. First, they can carry large amounts of DNA, thereby conferring a wide variety of important characteristics. Some of these traits are important for the biology of the bacteria that harbor them. For example the repABC plasmids in species of Agrobacterium can encode virtually all of the …
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Characteristics of an Endonuclease Associated with Adenovirus Types 2 and 12
… cells but abortively infects hamster cells. The metabolism of the DNA of these adenoviruses was determined in both human cells and hamster cells. The adenovirus DNA, both the parental DNA and the newly synthesized DNA, is found as three species within the cells. The species which sedimented …