Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 33 for “"DNA metabarcoding"”.
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Algal Biodiversity Survey of the Middle Chattahoochee River Using DNA Metabarcoding
… the naked eye, so researchers have been using DNA metabarcoding, where DNA belonging to specific taxa can be isolated and identified from water samples. The objective of this study was to conduct an algal biodiversity survey on the middle Chattahoochee River system in Georgia. Water samples …
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Sample Collection and DNA Extraction Methods for Environmental DNA Metabarcoding in Headwater Streams
<p>DNA is found to be free and ubiquitous in the environment where it is no longer associated with the source organism, and is also known as environmental-DNA (eDNA). Methods optimized for specific environments may be able supplement insight to local taxa richness. With the advent of high …
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The role of sampling location and depth in the performance of lake plant environmental DNA metabarcoding
… and to ecosystem management. Environmental DNA (eDNA) methods permit broad yet detailed biodiversity sampling to be performed with minimal field effort, but uncertainty remains regarding the spatial resolution necessary for effective sampling. We analyze eDNA samples from twelve temperate …
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Using environmental DNA metabarcoding to monitor fish biodiversity and assess anthropogenic barrier impacts on the river Mersey, UK
… use of a novel molecular approach, environmental DNA (eDNA) metabarcoding, is now revolutionising biomonitoring. In this thesis, data collected from non-invasive monitoring techniques (camera recordings and eDNA metabarcoding) were combined to monitor Atlantic salmon migration at the Woolston weir …
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Environmental DNA metabarcoding and citizen science as a cost-effective and rapid tool for monitoring terrestrial mammalian species
The use of environmental DNA (eDNA) has become increasingly important as a surveying technique for monitoring rare, elusive and/or invasive species. eDNA metabarcoding (which targets multiple species simultaneously) has been frequently used to monitor aquatic species but more recently, has been …
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Análisis de la diversidad fúngica en rizosferas de ecosistemas Cantábricos
… ello, mediante técnicas de análisis molecular (DNA Metabarcoding) se ha realizado una identificación completa de los hongos asociados a especies de pastizal que se desarrollan en zonas alpinas de la Cordillera Cantábrica y a robledales atlánticos localizados, también, a lo largo de la Cordillera …
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Nematodes as indicators of sustainable soil health management practices in corn-soybean production
… addressed knowledge gaps by combining enhanced DNA metabarcoding tools with the standard morphological analyses to evaluate soil nematode communities under different crop management practices. The first study evaluated the use of soil DNA for profiling nematode communities. Compared to the …
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The relationship between population dynamics and dietary preferences within two snake genera (Storeria and Thamnophis)
… and <em>Thamnophis</em> and analyzed DNA metabarcoding data from fecal samples. I found no significant difference in the dietary diversity or proportion of nonnative prey in the diets of <em>Storeria</em> or <em>Thamnophis</em>. The diet of S. occipitomaculata was dominated by …
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e(lasmo)DNA : the role of environmental DNA (eDNA) analysis in marine fish biodiversity assessment, with special focus on elasmobranchs
… detection is inherently difficult. Environmental DNA metabarcoding, based on the retrieval of genetic traces (skin cells, metabolic waste, etc.) naturally released in the environment, is emerging as a non-invasive method for the detection and identification of rare and elusive species in a wide …
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Development of Bioinformatic Tools and Procedures to Computationally Support DNA Barcoding
… investigating nucleotide alignments as part of DNA barcoding and DNA metabarcoding. In addition, the existing barcode regions were investigated to discover if possible mini-barcodes could be used in order to improve identification in a wide range of plant species. The pipelines developed in the …
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Foraging ecology and population structuring of baleen whales in the western South Atlantic and eastern South Pacific
… to feeding grounds. In addition, (iv) I combine DNA metabarcoding, population genetics and stable isotope analysis to compare foraging patterns, genetic diversity, and population structure of present-day and pre-exploited populations of the sei whale, Balaenoptera borealis, in the western South …
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Valuing the ecosystem services provided by UK bats under climate change
… multispecies bat assemblages across the UK using DNA metabarcoding and ecological network analysis. I found that bat communities consume a diverse range of arthropod prey, including numerous agricultural pest species. Pest predation emerged from complementary feeding strategies across bat …
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Eukaryotic Parasites of Toxic and Bloom-forming Diatoms: Molecular insights in their diversity and physiology
… have been investigated in the field via DNA metabarcoding, highlighting swift parasitic outbreaks and suggesting a role in the regulation of diatom blooms. The same method resulted in the first molecular identification of a transatlantic distributed chytrid infecting the spring …
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Stress eating? Causes and consequences of variation in foraging behavior in a challenging world
… control birds. In Chapter 2, I use fecal sample DNA metabarcoding to show that adult birds with experimentally elevated corticosterone had diets composed of a lower percentage of macronutrient-rich aquatic insects than control birds. Combined, the results of my first two chapters suggest that …
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Assessing risk to native ecosystems: using exotic ants as a model
… and co-occurrence patterns, as well as employing DNA metabarcoding, to understand what invertebrate taxa are most at risk via predation. Furthermore, I investigated the potential disruption of floral visitor networks by exotic ants through conducting surveys across the flowering plant community …
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Honey Bee Colony Resource Acquisition, Population Growth, and Pollen Foraging in Diversified Native Grass-Wildflower Grazing System
… though further research is needed. Pollen DNA metabarcoding revealed that honey bees in both diversified and conventional pasture systems have similar diets, and that sown species were foraged upon primarily in the fall. Samples collected from diversified pasture systems yielded greater …
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Metabarcoding pollen to identify allergenic species of grasses (Poaceae) and ragweed (Ambrosia spp.) across six monitored sites in South Africa
… the genus level. This project aimed to introduce DNA metabarcoding techniques to classify and identify grass and ragweed species contributing to pollen allergies in various South African biomes. Methods Samples were selected from weeks with the highest grass pollen counts between October 2022April …
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Metabarcoding pollen to identify allergenic species of grasses (Poaceae) and ragweed (Ambrosia spp.) across six monitored sites in South Africa
… the genus level. This project aimed to introduce DNA metabarcoding techniques to classify and identify grass and ragweed species contributing to pollen allergies in various South African biomes. Methods: Samples were selected from weeks with the highest grass pollen counts between October 2022- …
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Exploring the eco-hydrological and predator movement effects of floating roads in the context of forest to bog restoration in the Flow Country
… including scat counts, camera trapping, DNA metabarcoding, in-situ water table depth, and remote sensing (INSAR/APSIS), to evaluate their usefulness for monitoring a wide range of ecosystem effects associated with floating roads, and comment on the scope for road-to-bog restoration in the …
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A System-Level Analysis of Migratory Shorebird Habitat Use, Diet, and the Influence of Climate-Driven Ecosystem Change in the Virginia Barrier Islands
… (May 14 – June 2, 2023 – 2024) and used fecal DNA metabarcoding to examine diet and prey selection on mudflats. Mudflats supported comparable shorebird numbers and invertebrate densities to barrier island sand substrate. The composition of shorebird and invertebrate communities differed across …
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