Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"DNA melting"”.
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Mechanistic studies on transcription activation via DNA looping in a prokaryotic promoter-enhancer system
… the RNA polymerase at the promoter by means of DNA looping (closed complex) and induces DNA melting of the promoter DNA by RNAP-sigma54 (open complex). The following aspects of this process were studied: (1) Three different sigma54-specific promoter sequences were analyzed in binding studies and …
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Molecular typing of virulence genes in enterotoxigenic Bacillus cereus
… dye and its florescence increases as a result of DNA amplification during real-time PCR. A second-derivative plot is obtained at the end of the PCR run, where amplicons are differentiated based on their DNA melting temperature (Tm). DNA was extracted from Tryptic Soy Broth (TSB) and 2.5% Nonfat …
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PNA-Ligand Bioconjugates as Potential Building Blocks for Sequence-Specific, Metal-Mediated DNA-/RNA-Cleavage
… of artificial enzymes for sequence-specific DNA and RNA cleavage is one of the most challenging problems in modern biotechnology, since the commercially available biotools are restricted to a limited number of promoter sequences. Possible nuclease mimics consist of a recognition- and a …
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Structural and Computational Studies of RNA Polymerase
… details of bacterial promoter recognition and melting. In addition, our understanding is generally limited to highly conserved elements of the structure, with little focus on the bacterial lineage-specific domain insertions. Furthermore, we lack information about the hidden functional residue …
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Cryo-EM Studies of Bacterial RNA Polymerase
… and (4) Eσ<sup>70</sup>-dependent promoter DNA melting intermediates stabilized by the TraR transcription factor. Using cryo-EM, I captured RNAP structures that were intractable to crystallization, visualized multiple RNAP conformational states populated in solution, deconvoluted RNAP …
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Development of a T-Loop Assay to Investigate T-Loop Dynamics and Structure
… to the difficulty of isolating and visualizing DNA with intact loop structures. Thus, we have developed novel methods to isolate DNA such that biochemical assays and microscopic visualizations for authentic t-loops are now possible. Digestion of proteins that stabilize t-loop and significant …
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Nanoparticle Biofunctionalization for Self-Assembly and Energy Transfer Applications
… quantum dots (QDs), and quantum rods (QRs) with DNA and proteins for self-assembly, energy transfer and drug delivery applications.</p> <p>In the first project, we explored the ability to assemble QDs into clusters with a high degree of control through DNA-mediated interactions. The hydrophobic …
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Properties of Nanoscale Biomaterials for Cancer Detection and Other Applications
… [3]. Finally, a novel method for measuring the melting point of DNA in solution using capacitance measurements is presented. This method allows for the determination of the melting temperature as well as the melting entropy and melting enthalpy of DNA strands. Two different short strands of DNA, …