Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"DNA isolation"”.
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Identification of endophytic bacterial communities associated with South African crops: sorghum bicolor (L. Moench), pennisetum glaucum and arachis villosulicarpa
… study was to investigate the effect of different DNA extraction protocols on mDNA yield and quality, as well as the diversity of endophytic bacteria retrieved from root and stem tissues (0.1g or 0.3g) of sorghum, pearl millet and groundnut. Protocols used include two classical methods (CTAB- and …
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Detection of Genetic Thrombophilia (Fa V Leiden & PT20210 mutation) using GeneXpert Technology with IQCP Implementation
… PCR method, it involves individual processing of DNA isolation, amplification, and detection using three (3) different instruments resulting to an increased turnaround time of 5 to 7 days and additional staff utilization. It is performed by repetitive manual sample pipetting and preparation of …
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Určování pohlaví kalouse ušatého (Asio otus) na základě PCR amplifikace DNA
… the sex of captured Long-eared Owl by DNA isolation from blood platelets in the bird quail and subsequent PCR reaction. This thesis also describes the PCR method, which is one of the most used methods in the todays laboratories of many different field of science. I also mention the …
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Defect of Mitochondrial Translation Machinery Enhances Hepatoma Cell Invasiveness
… and mitochondrial ROS level 5 5. Total genomic DNA isolation and sequencing of mitochondrial DNA fragments 5 6. Western blot analysis 6 7. Real-time reverse transcription-polymerase chain reaction (qPCR) 7 8. Gene expression profiling and data analysis 7 9. siRNAs transfection. 8 10. Cell …
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SURVEY OF Rickettsia spp. IN TICKS IN NACOGDOCHES COUNTY, TEXAS
… county and taxonomically identified. The DNA was extracted using DNeasy Blood and Tissue Kit, and the UltraClean Microbial DNA Isolation Kit. The genomic contents of the tick were subjected to PCR amplification to identify <em>Rickettsia </em>genus bacteria. Any ticks testing positive for …
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T-RFLP analysis of bacterial 16S rRNA gene sequences isolated from river otter (Lontra canadensis) scat and parasite screening for the presence of Toxoplasma gondii
… Cosumnes River Preserve (Sacramento County, CA). DNA was isolated from each sample with the MOBIO PowerSoil™ DNA Isolation Kit and 16S rRNA gene sequences were amplified from each sample. After digestion with Mspl, TRFLPs were analyzed in an ABI Prism™ 310 Genetic Analyzetin triplicate and data …
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DNA Fingerprinting: Identification of Organisms Using the Polymerase Chain Reaction and Various Primers
The study undertaken involved small scale DNA isolation from eight different fruits using a modified technique written for leaf material. Genetic analysis of this extracted DNA was performed by PCR. Four primers known to target specific DNA sequences were utilized: Analu, Bactoribo, HHFl, and …
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Molekulinė naminių pelėdų (Strix aluco) hemosporidinių parazitų diagnostika /
… from Kaunas Tadas Ivanauskas Zoological Museum. DNA isolation with ammonium acetate and nested PCR revealed that 27 out of 76 individuals were infected with parasites. After sequencing, the parasites were identified using BLAST algorithms in the NCBI genebank and MalAvi database. The study showed …
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Validation of a Pan-fungal polymerase chain reaction (PCR) assay for the detection and identification of medically important fungi in a diagnostic laboratory
… based on the microscopic detection, culture isolation, and detection of serological response. In recent years, there has been an increase in the utilization of molecular diagnostic techniques for the detection and accurate identification of fungal pathogens. This was a laboratory accuracy …
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The Use And Development Of Laser Microdissection To Separate Spermatozoa From Epithelial Cells For Str Analysis
… in cell separation methods from mixtures for DNA typing. This report describes the use of laser microdissection (LMD) for the separation of pure populations of spermatozoa from two-donor cell mixtures. In this study, cell separation was demonstrated by microscopic identification of …
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Analysis of the Relationship Between Growth Hormone Receptor Polymorphism rs6180 and Craniofacial Morphological Changes Associated with Herbst Appliance Therapy
… cheek swabs. The cells obtained have undergone DNA isolation with the Puregene method in microcentrifuge tubes (Gentra Systems, Minneapolis, MN). Upon termination of functional appliance therapy (post-Herbst), a final lateral cephalometric radiograph was taken. To analyze the genetic …
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Historical Genetic Analysis Of Horned Lark (Eremophila Alpestris) Specimens From The Prairie Pothole Region
… and identified three distinct mitochondrial DNA clades. This study aims to genetically characterize Horned Larks from the North Dakota region using the mtDNA NADH dehydrogenase subunit 2 (ND2) gene. Horned Larks were sampled from museum collections, with ages ranging from 13 - 120 years old. …
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Characterization of psb O mutants from cyanobacterium synechococcus PCC 7942 and expression of the wild-type gene in escherichia coli
… or mutant MSP is not toxic to the cells. Plasmid DNA isolation and restriction analyses of several of the mutant clones also confirmed the presence of the correct size inserts in the vector. However, upon sequencing several mutant clones, it appeared that losses and/or rearrangements of sequences …
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Identifying parasite virulence factors and host genetic and immunologic factors that contribute to severe malarial outcomes in Ugandan children
… and stored accordingly for future RNA and DNA isolation. We performed genotyping for endothelial protein C receptor (EPCR) polymorphisms, quantitative reverse-transcriptase PCR to estimate transcript levels of var genes encoding P.falciparum erythrocyte membrane protein 1 (PfEMP1), and used …
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Development of Bioassays Based on Nucleic Acid Amplification and Next-Generation Sequencing
… the nuclear ribosomal ITS2 region of plant DNA, which is known to support genus-level discrimination. We purified pollen-free DNA from honey, filtered or centrifuged to remove all pollens, using three different methods: (i) anti-dsDNA antibodies coupled to magnetic particles; (ii) Q …
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A Comprehensive Evaluation of a new Direct Amplification System (PowerPlex® 18D) in Forensic DNA Profiling
… tandem repeat typing is the primary method of DNA identification used in the field of forensic science. Over the past several years the need to improve on this method has moved to the forefront of research. Due to the increasing number of criminal cases and the substantial backlogs most …
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Identifikasi pola haplotipe DNA mitokondria udang jari (Metapenaeus elegans) Segara Anakan Kabupaten Cilacap Jawa Tengah menggunakan enzim restriksi HindIII
… adalah dengan mengidentifikasi pola haplotipe DNA mitokondria menggunakan enzim restriksi HindIII. DNA mitokondria hanya diturunkan dari induk betina dan memiliki variasi yang tinggi. Enzim restriksi HindIII telah digunakan dalam penelitian Klinbunga dkk (1998) dan memotong banyak fragmen pada …
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Studi pengujian metode isolasi DNA dari spesimen herbarium Rafflesia
… teknologi penyimpanan deoxyribonucleic acid atau DNA. Penyimpanan DNA diharapkan dapat mempertahankan kekayaan hayati yang terkandung di dalam suatu tumbuhan selama ratusan tahun. Mengumpulkan material segar Rafflesia adalah sulit, dan tidak selalu mungkin untuk mengumpulkan spesimen Salah satu …
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Application of Single-Molecule Analytical Methods for the Characterization of Multimeric Genetic Elements in Yarrowia lipolytica DSM 3286
A thorough examination via DNA sequencing of multimeric genetic elements or random integration events in production cell lines or in potential host genomes is a critical regulatory component in the assessment of potential health risks associated with therapeutic proteins and gene therapeutics. …
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Vaginal microbial diversity of the genital tract of South African adolescent females
… Clinic in Cape Town, South Africa. Bacterial DNA was extracted from lateral vaginal wall swabs using the MoBio Powersoil® DNA Isolation Kit after enzymatic digestion. Positive bacterial reference strains were cultured in MRS buffer and Schwedler's broth, after which the DNA was extracted using …
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