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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"DNA hydrolysis"”.
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DNA hydrolysis and genome editing applications of GIY-YIG family homing endonucleases
… are most efficient when a double-strand DNA break is introduced at the loci where the modification is desired. Different classes of naturally occurring DNA endonucleases, including homing endonucleases, have therefore been explored as candidates for genome modification studies as they …
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Generation of chickenized 3D8 scFv antibody from mouse monoclonal anti-DNA antibody by CDRs-grafting
… that has ability of cellular internalization and DNA/RNA-hydrolyzing activity. It has been reported that cells treated with 3D8 scFv protein showed the down-regulation against replication of RNA-genomed viruses and this anti-viral activity should be due to the cellular internalizaing and DNA/RNA- …
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Deoxyribozymes to hydrolyze DNA phosphodiester bonds
In nature, the main role of double-stranded DNA is storage of genetic information. However, once a DNA oligonucleotide is freed from its confining complementary strand, it can fold into complex three-dimensional structures that support catalytic activities. These catalytic single-stranded DNA …
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Optimisation of DNA extraction from teeth submerged in sea water in False Bay, South Africa
Extracting forensically useable DNA from human remains recovered from an open marine environment is problematic, and in some cases, impossible. The reason is unclear given the lack of research on marine decomposition, DNA survival in seawater, and possible methods to optimise the DNA extraction …
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The Development of New Tools to Investigate Alphavirus Replication Kinetics
… reverse-transcription. We also determined that DNA hydrolysis probes increased the sensitivity of ssqPCR assays when compared to a double-stranded DNA-specific dye, SYBR Green. Using this information, we determined the replication kinetics of two different genotypes of o'nyong nyong virus (ONNV) …
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Bioinspired Catalysts for Phosphoryl Transfer Reactions
… enzymes, including the exonuclease subunit of DNA polymerase I, a mechanism involving two metal ions and double Lewis-acid activation of the substrate, combined with leaving group stabilisation, has been proposed. Aim of this thesis was the study of low-molecular-weight models of phosphoryl …
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Catalysis by DNA enzymes: roles of lanthanide(III) ions and DNA aptamer modules
For many years scientists considered DNA and RNA to be merely the means for the storage and transmission of genetic information. Catalytic roles of some RNA molecules, called ribozymes, were discovered in the early 1980s. Considering structural similarities between RNA and DNA, it was reasonable to …
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Identifizierung und Charakterisierung von thermostabilen Uracil Glykosylasen von Archaeon Methanobacterium thermoautotrophicum und Bakterium Thermus thermophilus
… Uracil und T/G, U/G "Fehlpaarungen" spezifischen DNA-Glycosylasen postuliert. Mit dem Ziel das Wissen über U/G und T/G entfernende Enzyme zu vertiefen, lag der Fokus dieser Arbeit in der Isolierung und enzymatischen Charakterisierung unterschiedlicher und neuartiger Uracylglycosylasen zweier …