Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 52 for “"DNA hybridization"”.
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DNA Hybridization and Ligation for Directed Colloidal Assembly
… slow centrifugation. The salt requirement for DNA hybridization and ligation made it necessary to use slow salt addition through a dialysis membrane to achieve well-ordered colloidal crystals. Well-ordered hexagonally close-packed polystyrene colloid monolayers could be assembled by …
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DNA hybridization : fundamental studies and applications in directed assembly
Programmed self-assembly using non-covalent DNA-DNA interactions is a promising technique for the creation of next-generation functional devices for electronic, optical, and magnetic applications. This thesis develops the ability to tailor surfaces for the DNA-driven assembly of molecular, nano-, …
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New nitride-based devices for electrical detection of DNA hybridization
… new solid state sensors for the detection of DNA hybridization through electrical measurements. The first sensor is a surface acoustic wave device with tapered IDTs capable of giving us a tomographic image of the functionalized sensor surface. We investigated using an array of these type …
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A Sensor for DNA Hybridization Using Fluorescence and Electrical Impedance Detection
… has been established in this work. This basic DNA biosensor design involves the immobilization of thiolated single-stranded (ss) oligonucleotides (20-mer probe; 1.0 æM) on gold 7 mm half-moon transducer surfaces (with a 0.5 mm gap) to recognize its complementary (target) DNA sequence by …
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Characterization and optimization of PDMS microfluidic devices for rapid DNA hybridization
… of conducting rapid, flow-based, multiplexed DNA hybridization. Experimental results showed that flowing hybridization assays could detect similar concentrations of labeled probe as standard stationary microarrays, but in 1/100h of the time, using 2% of the sample volume. An 8-channel device …
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Design and construction of an apparatus for induction heating for controlling DNA hybridization
… nanoparticles attached to "molecular beacons" or DNA loop/hairpin structures. Testing was conducted to see if the heat would be sufficient to open the molecular beacon by dehybridizing the dsDNA. This was accomplished by developing a series of seven coils that were tested using a network analyzer …
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Biotin and Fluorescent Labeling of Ribonucleic Acids Using T4 Rna Ligase (Rna Modification, Rna-Dna Hybridization)
The biotin, fluroescein, and tetramethylrhodamine derivatives of P('1)-(6-aminohex-1-yl)-P('2)-(5'adenosine) pyrophosphate were synthesized and used as substrates with T4 RNA ligase. In the absence of ATP, the non-adenylyl portion of these substrates is transferred to the 3'-hydroxyl of an RNA …
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Charge-Modulated Field-Effect Transistor: technologies and applications for biochemical sensing
… investigated in literature: in particular, DNA hybridization detection represents a basic biological reaction for several, more sophisticated analysis in medical, pharmaceutical and forensic fields. The development of the research activity was centered on a specific biosensor, namely …
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DNA Biosensors Based on Gold-Coated Magnetic Nanoparticles
The interaction between DNA-functionalized surfaces/nanoparticles and free oligonucleotides in solution is the foundation of many devices such as DNA gene chips, therapeutic devices, and DNA biosensors. Electrochemical transduction provides a convenient means for studying DNA hybridization due to …
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MicroCantilever (MC) based nanomechanical sensor for detection of molecular interactions
… associated with: self-assembly of alkanethiol, hybridization of ssDNA, and the formation of cocaine-aptamer complexes were measured. Dissociation constant (Kd) for each molecular reaction was utilized to estimate the surface coverage of affinity complexes. In the cases of DNA hybridization and …
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A taxonomic re-appraisal of the Passeriformes (Aves) based on the morphology of the plantar surface of the foot
… studies, and more recent works (particularly DNA-DNA hybridization), is given. The present study attempted to analyse little used characters, i.e. those on the plantar surface of the foot, of over five hundred species of bird. Large amounts of variation and homoplasy were found. Compatibility …
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Application of molecular biology techniques in the assessment of microbial community responses to environmental perturbations
… density of representative taxa were evaluated by DNA-DNA hybridization of prokaryotic communities at stations above and below points of industrial effluent release. Preliminary studies in 1990 indicated a positive correlation (0.95) between traditional identification and enumeration and DNA-DNA …
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Genetic analysis of Ximenia Americana under natural conditions
Cross-species hybridization approaches have been used for genetic analysis of species that do not have complete genome arrays available. The study used the cross-species DNA-DNA hybridization approach to characterize Ximenia americana biological processes. Preliminary micro-array studies done in …
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Evolutionary genetics and systematics of fungi in the Collybia dryophila group
… populations was measured by the method of DNA hybridization. In general, there was good correspondence between mating compatibility and base sequence homology in the <i>C. dryophila</i> group. Allopatric, intercompatible populations, however, showed reduced homology when compared to that …
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Studies on the Vibrio alginolyticus sucrose utilization system cloned into Escherichia coli
… its insert determined by Southern blotting and DNA hybridization. The number and sizes of the polypeptide products encoded by plasmid pVSlOO were determined by DNAdirected cell-free protein synthesis. The capsule, produced by transformed and untransformed E. coli JA221 cells, was shown to be …
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Development of New Tools for Functional Genetics
… been applied to the photochemical regulation of DNA hybridization, DNAzyme catalysis, antisense gene regulation, RNA folding, and protein function.
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DIRECT REPEATS AND DELETIONS IN MITOCHONDRIAL DNA: CASUAL AND EVOLUTIONARY ASPECTS
… to understand the role of sequence motifs and DNA misalignments in mitochondrial DNA (mtDNA) deletion mutagenesis. Meta-analysis of age-related deletion breakpoints indicated that in human and rhesus monkey but not in mouse and rat, the average distance observed between a mtDNA breakpoint and …
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DNA-DNA Checkerboard Hybridization to Identify Bacteria in Human Plaque Samples
<p>Previous research has shown that using DNA probes to identify the presence of specific bacteria in human plaque samples is superior to the commonly used anaerobic cultural procedures. Some periodontal pathogens are either uncultivable or very difficult to grow, such as<em> Prevotella intermedia, …
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Iron Acquisition in <em>Rhodococcus erythropolis</em> Strain IGTS8: Characterization of a Mutant that Does Not Produce a Siderophore.
… N5-59 is not defective in siderophore uptake. A DNA hybridization probe was prepared using DNA flanking the site of insertional mutation in strain N5-59. This probe was then used to clone a 6 kilobase pair, <em>Pst</em>I restriction fragment from the chromosome IGTS8. This cloned DNA is expected …
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PNA-protein conjugates for nano-scale modeling of protein aggregates
… protein assemblies directed by precise PNA-DNA hybridization. The signatures of assembly were studied via FRET, providing a powerful tool which should be effective in live system imaging. Two model systems were developed in this study. In the first model system, site-selective conjugation of …
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