Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 59 for “"DNA fragmentation"”.
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Aneuploidy and DNA fragmentation in morphologically abnormal sperm
… the connection between sperm morphology and DNA integrity. Sperm morphology will be evaluated on its ability to identify the level of chromosomal abnormalities or fragmented DNA in sperm. To further focus this investigation on sperm morphology, men with infertility isolated to abnormal sperm …
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Patterns of post-translational histone modifications, chromatin condensation and DNA fragmentation during apoptosis
… Fragmentierung des Chromatins durch die über die DNase CAD (DFF40) katalysierte Spaltung nucleosomaler DNA deutlich zu erkennen. Des Weiteren haben post-translationale Histonmodifikationen wie Azetylierung, Methylierung und Phosphorylierung großen Einfluss auf die Bildung kompakter …
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Untersuchungen zur Apoptose durch das Kontakthapten NiCl 2 in humanen Nabelschnurendothelzellen
… ein; dieser blockierte die NiCl/CHX-vermittelte DNA-Fragmentation und Apoptose vollständig. Im Rahmen physiologischer wie auch pathophysiologischer Vorgänge werden Endothelzellen oxidativem Streß in Form reaktiver Sauerstoffradikale ausgesetzt, die zu einer Heraufregulation von Fas und seines …
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Ecotoxicology of marine biotoxins in bivalve shellfish
… indicated a chemically-induced response to OA. DNA fragmentation was measured in the haemolymph and hepatopancreas cells post OA-exposure in Mytilus edulis and Crassostrea gigas. A significant increase in DNA fragmentation was observed in both species over time, even at the lowest OA …
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Investigation of the genotoxic potential of the marine biotoxins okadaic acid and azaspiracids
… investigate genotoxicity in the present study, DNA fragmentation was detected using the COMET assay. In contrast to most other available studies, two further endpoints were included. The Trypan Blue Exclusion assay was used to provide information on possible cytotoxicity and assess the right …
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Comparative evaluation of different extenders of bull semen stored under different conditions
… 48 and 72 hours. Morphology, viability and sperm DNA fragmentation were analysed after 72 hours. The study was replicated four times and data was analysed by ANOVA. Triladyl had higher sperm viability rate and total motility rate for 72 hours (P<0.01). However, Ham’s F10 had higher progressive …
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Mammalian cell cultures as a model system for the determination of cytotoxicity induced by cholesterol oxidation products
… increase in caspase-3 activity in HepG2 cells. DNA fragmentation is regarded as the biochemical hallmark of apoptosis, therefore the comet assay as a measure of DNA fragmentation was assessed as a measure of apoptosis. The level of DNA fragmentation induced by 7β-OH, as measured using the comet …
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The role of the Bcl-2 family in proliferation and apoptosis and in mediating the development of lymphatic diseases
… of apoptosis in any given cell; these include DNA fragmentation and chromatin condensation, "blebbing" of the plasma membrane, and subsequent phagocytosis of the resulting cell fragments by adjacent cells (Kerr et al. 1972 and Wyllie et al. 1980).
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Oxidative Stress Based Strategies For Enhancing The Efficacy of Histone Deacetylase Inhibitors (Hdaci)
… potential for both combinations. Interestingly, DNA fragmentation and caspase-3 activity was only blocked by NAC in cells treated with vorinostat-adaphostin; but not with entinostat-adaphostin. These results suggest that different redox mechanisms are involved in the induction of ROS-mediated …
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Nanosecond Pulsed Electric Field Effects on Cell Cycle and Apoptosis
… plasma membrane, caspase protease activation, DNA fragmentation, release of cytochrome c from the mitochondrion, and membrane blebbing. An important regulatory protein in the apoptotic pathway is p53. The p53 protein functions to modulate the cell cycle by arresting cells in the G1 and G 2 …
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Protective effect of Nicotinamide on High glucose (HG)/Palmitate (PA)-induced Glucolipotoxicity to INS-1 beta cells
… viability reduction and HG/PA-induced DNA fragmentation. Knock down or overexpression of CD38 did not protect HG/PA-induced viability reduction and HG/PA-induced Caspase3 induction. Resveratrol, SIRT activator, slightly augmented HG/PA-induced cell death. But Sirtinol, SIRT inhibitor …
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Relationships between in vivo and in vitro heterospermic ranking, embryo development, and sperm characteristics of Holstein and Jersey bulls
… CASA) and viability (flow cytometric analysis of DNA fragmentation, acrosome integrity, plasma membrane integrity and disorder, and intracellular Ca2+), or standardized to equal numbers of motile sperm and recombined 1:1 resulting in the 9 H/J pairings for heterospermic IVF (H-VITRO-2). A …
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<i>In Vivo</i> Murine Melanoma Tumor Responses to Nanosecond Pulsed Electric Field Treatment
… on the melanomas <em>in vivo</em>, ranging from DNA fragmentation, caspase activation, nuclear damage, apoptosis induction, damage to pre-existing infra-tumoral vessels and neovascular inhibition. These tumor responses were expressed by histological and biochemical changes in both short and long …
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Trasduzione del segnale e poliamine nell'apoptosi di cellule cardiache
… of the release of 14C-CO2 from 14C-ornithine. DNA fragmentation was visualized by the method of terminal transferase-mediated dUTP nick end-labeling (TUNEL), and DNA laddering on agarose gel electophoresis. Cytochrome c was detected by immunoflorescent staining. Activation of signal …
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Molecular regulation of neuronal apoptosis in PC12 cells.
… apoptosis were examined by RT-PCR. Cell death, DNA fragmentation and morphological changes associated with apoptosis were evident within 8 hours after exposure to VM26 indicating that VM26 is capable of inducing apoptosis. Within 8 hours of VM26 exposure, clusterin (TRPM-2) and IGFBP2 …
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Induction of Apoptosis and Necrosis By Infectious Pancreatic Necrosis Virus in Chinook Salmon Embryo Cells
… Hoescht 33256 fluorometric analysis of isolated DNA. Data indicate that CHSE-214 cells are capable undergoing spontaneour apoptosis. Furthermore, IPNV (LWVRT) induces severe necrosis by 16 hr post-inoculation and mild apoptosis by 24 hr post-inoculation as indicated by epifluorescence microscopy. …
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Synergic Effect on Cell Death by Co-treatment with TRAIL and Human Anti-DR5 Antibody
… were dying with both apoptotic (blebbing and DNA fragmentation) and autophagic features (autophagosome containing organelles). Analyses of each signaling markers (apoptosis; PARP cleavage and autophagy; increased LC3-II form) induced by TRAIL, HW1 and combination of them showed that the …
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Investigation and validation of mammalian telomeres as biomarkers for health and disease
… TL, with a specific focus on the effects of DNA extraction methodology, tissue-specific telomere dynamics, and telomere-specific oxidative damage. A mouse adapted monochromatic multiplex qPCR (mmqPCR) assay was developed and validated to measure relative TL, complemented by a …
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Investigations on the effects of Typha capensis on male reproductive functions
… (determined by means of Annexin V-Cy3 binding), DNA fragmentation (determined by means of the TUNEL assay). Part 3: NMR spectrometric analysis was performed on a 13C spectra were recorded at 400 MHz. TM3-Leydig cells and LNCaP cells incubated with different concentrations of bioactive compounds …
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