Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 10 of 10 for “"DNA enzyme"”.
-
The Effect of Skewed Elevation in dNTP pools on Loss of Heterozygosity in Saccharomyces cerevisiae
DNA precursors, deoxyribonucleotide triphosphates (dNTP), are the basic components of DNA. Enzyme ribonucleotide reductase (RNR) tightly regulates the levels of dNTP pools to ensure high fidelity DNA replication and repair. Misregulated levels of dNTP pools cause mutagenesis which impacts genome …
-
From Micro- to Nano-Scale: Applications of Solid-Phase Enzymatic Reactors for Biopolymer Disassembly
<p>The process of immobilizing enzymes onto solid supports for bioreactions has some compelling advantages compared to their solution-based counterpart including the facile separation of enzyme from products, elimination of enzyme autodigestion, and increased enzyme stability and activity. We …
-
DNA catalysts for amide bond cleavage and for lysine side chain modification
… is known to exploit both proteins and RNA as enzymes. No natural enzymes, however, have been discovered that are made of DNA. One can think of both RNA and proteins as large biopolymers with the potential to form complex secondary and tertiary structures capable of doing catalysis. Given the …
-
Catalysis by DNA enzymes: roles of lanthanide(III) ions and DNA aptamer modules
For many years scientists considered DNA and RNA to be merely the means for the storage and transmission of genetic information. Catalytic roles of some RNA molecules, called ribozymes, were discovered in the early 1980s. Considering structural similarities between RNA and DNA, it was reasonable to …
-
DNA enzymes for tyrosine PEGylation and azido-adenylylation of peptide and protein substrates
Proteins and RNA are used as enzymes in nature, while DNA is used for the storage and transfer of genetic information. Proteins and RNA are biopolymers that can fold into complex secondary and tertiary structures to enable substrate binding and catalysis. Given the structural similarity to RNA, …
-
The Diversion of Macromolecular Synthesis in L-Cells Towards Ends Dictated by Mengovirus
… viruses have provided much knowledge about the enzymes involved in bacteriophage multiplication and the physiological changes wrought in bacteria by infection (Cohen, 1961; Kornberg, 1961). In attempting to provide comparable information about mammalian viruses, we must of necessity follow their …
-
Nucleobase-modified deoxyribozymes for amide and peptide hydrolysis
Nature has evolved proteins and RNA as enzymes. These biopolymers can fold into complex structures to enable catalysis. DNA is primarily double-stranded and is non-catalytic in nature. However, considering the structural similarity to RNA, single-stranded DNA should also be able to form complex …
-
In vitro selection of monovalent metal ion-dependent DNAzymes
DNA is no longer thought of as only the purveyor of genetic information, but also as a highly programmable and functional biopolymer. It was discovered in 1994 that some single-stranded DNA sequences can fold into complex tertiary structures to catalyze reactions, these DNA sequences are called …
-
Cytochrome c-DNA and cytochrome c-enzyme interactions for the construction of analytical signal chains
… surfaces. With the help of the biopolymer DNA, the redox protein assembles into electro active multilayer (ML) systems, providing a biocompatible matrix for the entrapment of proteins. In this study the characteristics of the cyt c and DNA interaction were defined on the molecular level for …
-
Biophysikalische Charakterisierung und biochemische Steuerung von Methyltransferase-DNA-Wechselwirkungen
… biophysical methods for the characterisation of DNA methyltransferase (MTase) interactions with DNA and their methodical direction by means of biochemical approaches. Taking the MTase M.TaqI as a paradigm, the kinetic analysis of these interactions is performed by stopped flow measurements with …