Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 21 for “"DNA ends"”.
-
HMO2, a yeast HMGB protein that preferentially binds to DNA ends
DNA damage is a common hazard that all cells have to combat. Saccharomyces cerevisiae HMO2 is a high mobility group protein (HMGB) that is a component of the chromatin remodeling complex INO80, which is involved in double strand break repair. I show here using DNA end-joining and exonuclease …
-
Characterization of a Novel 53BP1-Dependent Mechanism that Promotes Non-Homologous End Joining of Deprotected Telomeres by Increasing Chromatin Mobility
… the phosphorylation of H2AX) and the binding of DNA damage response factors (e.g. MDC1, 53BP1). Although several lines of evidence have pointed to a role for some of these factors in DSB repair through non-homologous end-joining (NHEJ), the mechanism of their contribution has not been …
-
Single-molecule studies reveal mechanisms of human DNA double-strand break repair
DNA damage is ubiquitous to all organisms and very complex pathways have evolved to recognize and repair these lesions. The most deleterious DNA damages are double-strand breaks (DSBs), and a single unrepaired DSB can lead to cell death. In human cells, there exist two canonical pathways of DSB …
-
Prediction and Investigation of Novel Proteins in DNA Double Stranded Break Repair
DNA double stranded breaks (DSBs) are the most genotoxic forms of DNA lesions, causing fragmentation of the DNA strands. Mis-repaired and unrepaired DSBs lead to chromosomal rearrangement and genomic instability promoting tumorigenesis. DSBs are primarily repaired by two independent and highly …
-
ARTEMIS AND METNASE MEDIATED PROCESSING OF 3΄-BLOCKED DNA LESIONS: ROLE IN RADIO/CHEMORESISTANCE AND DNA REPAIR
<p>DNA double-strand breaks (DSB) with chemically modified end-termini are the most significant lesions resulting from radio/chemotherapeutic intervention of cancer and non homologous end-joining (NHEJ) factor Artemis nuclease has been implicated in the repair of such breaks. To examine whether the …
-
Identification of MMS22 as a regulator of DNA repair
Obstacles such as DNA damage can block the progression of DNA replication forks. This is a major source of genome instability that can lead to cell transformation or death. The budding yeast MMS1 and MMS22 genes were identified in a screen for mutants that were hypersensitive to DNA alkylation that …
-
Identification and molecular characterisation of a putative gene for cell detaching factor from Trichomonas vaginalis.
… of the protein and its expression. A cDNA clone (CDF-2) was identified in a Tv cDNA library by immunological screen with rabbit anti-serum prepared against a purified preparation of CDF. This clone contained an open reading frame (ORF) that was believed to represent a partial coding …
-
Biochemical, biophysical and structural study of the nucleosome-MeCP2 complex
… as a protein that recognizes the genetic DNA methyl-CpG mark and it was thought to repress gene transcription by recruiting histone deacetylases. Recent studies show that MeCP2 can both repress and activate gene transcription. It also binds chromatin in the absence of the methylation mark, …
-
Structural and functional effects of histone variant, H2A.Bbd, on the nucleosome core particle
In eukaryotic cells, DNA is packaged into a protein-DNA assembly called chromatin. The basic subunit of chromatin is the nucleosome core, which is composed of 147 base pair (bp) of DNA wrapped in 1.65 turns around a histone octamer containing two copies each of the four core histone proteins (H2A, …
-
Development of Natural Cyclic Peptide Inhibitors of XRCC4/XLF Interaction for Radio-Sensitization of Breast Tumor Cells
… after exposure to radiation results largely from DNA double-strand breaks (DSBs). There are two main mechanisms in mammalian cells responsible for repairing the DSBs; the primary mechanism is non-homologous end joining (NHEJ) and the secondary mechanism is homologous recombination (HRR). Previous …
-
Pathways of NHEJ at Dysfunctional Telomeres and Their Resolution
… is a multiprotein complex that prevents DNA damage signaling at chromosome ends. In its absence, DNA repair pathways are activated that can promote the fusion of dysfunctional telomeres resulting in chromosomal instability. The work presented here aims to understand how telomeres are …
-
Optimisation of TrAEL-seq to study DNA damage and replication in complex and dynamic mammalian cell systems
… evolved a complex set of mechanisms to ensure DNA is repaired and correctly replicated before cell division. Deficiencies in these repair pathways are heavily associated with the development of cancer and ageing, and it is therefore of interest to be able to detect and monitor the distribution …
-
Regulation of nucleosome dynamics
… called nucleosomes, where 147 base pairs of DNA are wrapped around a protein core. Stable packing of DNA in nucleosomes imposes a barrier for accessibility of genetic code on DNA for replication, transcription, and repair. The dynamics of nucleosomal DNA provide a mean for gene regulation by …
-
Functional Analysis of the Yku Complex in Telomere Length Regulation
… the Ku heterodimer is required for the repair of DNA double strand breaks (DSBs) via nonhomologous end-joining (NHEJ). Interestingly, Ku has been shown to bind to the native chromosome ends. It contributes to the maintenance of wild type telomere length and, moreover, has been implicated in the …
-
Structural and biophysical analysis of Human DNA repair protein CtIP
… by endogenous and exogenous sources of DNA damage. The successful repair of DNA lesions is necessary for cellular survival and prevention of disease such as cancer. Therefore, complex molecular pathways have evolved to allow for accurate and timely DNA repair. It is now well-established …
-
Biochemical, Structural, And Drug Design Studies Of Multi-Drug Resistant Hiv-1 Therapeutic Targets
… model in complex with raltegravir and the viral DNA ends, identifying unique alterations in non-bonded interactions between the protein, DNA, and raltegravir as a result of the drug resistant mutations. </p> <p>The results of this work provide detailed structural information on HIV-1 protease and …
-
Dna Polymerase Θ (Polq) and The Cellular Defense Against Dna Damage
<p>In mammalian cells, DNA polymerase θ (POLQ)<strong> </strong>is an unusual specialized DNA polymerase whose <em>in vivo</em> function is under active investigation. The protein is comprised of an N-terminal helicase-like domain, a C-terminal DNA polymerase domain, and a large central domain that …
-
Mechanisms of Telomere End Protection by TRF2
… a six-subunit protein complex that protects the ends of mammalian chromosomes, called telomeres. The six subunits of shelterin, TRF1, TRF2, TIN2, TPP1, POT1, and Rap1, each have distinct roles in telomere homeostasis. These include telomere length regulation and preventing telomeres from …
-
An exploration of the interplay between HSV-1 and the non-homologous end joining proteins PAXX and DNA-PKcs
DNA damage response (DDR) pathways are essential in maintaining genomic integrity in cells, but many DDR proteins have other important functions such as in the innate immune sensing of cytoplasmic DNA. Some DDR proteins are known to be beneficial or restrictive to viral infection, but most remain …
-
On the Second Telomere Maintenance Machine: Structure, Recruitment, and Regulation of the CST–Polα/primase C-strand Fill-in Complex
… that safeguard genome integrity. Human telomeric DNA consists of double-stranded (ds) 5'-TTAGGG-3' repeats terminating in a 3' single-stranded (ss) overhang of the G-rich strand.The sequence and structure of the telomeric DNA is recognized and bound by the six-subunit shelterin complex, which …
Page 1 of 2