Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 4 of 4 for “"DNA endonuclease"”.
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An integrated CRISPR-Cas toolkit for engineering human cells
… Pyogenes comprising of the RNA-guided DNA endonuclease Cas9 and the guide RNA (gRNA) has been demonstrated to enable unprecedented genome editing efficiency across all domains of life. Current applications however, employ CRISPR/Cas technology in a stand-alone fashion, isolated from the …
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Investigation of Trans-factors responsible for the developmentally linked instability of a highly regulated mRNA in Trypanosoma brucei
… by RNAi. In addition expression of RNA-guided DNA endonuclease Cas9 was performed in *T. brucei* and the CRISPR system was assessed for future forward genetic screens, the results of which were highly encouraging. The first method assessed involved single target knockout by targeted Cas-9 …
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Engineering of transcription activator-like effector nucleases (TALENs) for targeted genome editing
… a pre-determined genomic locus by a rare-cutting DNA endonuclease. Subsequent repair of this DSB by non-homologous end joining or homologous recombination generates the desired genetic modifications such as gene disruption, gene insertion, gene correction, etc. For this purpose, I have constructed …
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Engineering Zymomonas mobilis for the production of biofuels and other value-added products
Zymomonas mobilis is a promising organism for lignocellulosic biofuel production as it can efficiently produce ethanol from simple sugars using unique metabolic pathways. Z. mobilis displays what is known as the “uncoupled growth” phenomenon, meaning cells will rapidly convert sugars to ethanol …