Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 111 for “"DNA double strand breaks"”.
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Repair of DNA Double-Strand Breaks in Chromosomes: Impact of Telomerase
No abstract prepared.
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ATM-Dependent ERK Signaling in Response to DNA Double Strand Breaks
… triggers many signaling pathways stemming from DNA damage, and, independently, from extra-nuclear events. To generate radio-mimetic DNA double-strand breaks (DSBs) without and minimizing the effects on extra-nuclear radiation targets, human (p53+) glioma and carcinoma cells containing …
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The Role of E2F1 In The Response to Dna Double Strand Breaks
… and stabilized in response to various forms of DNA damage to regulate the expression of cell cycle and pro-apoptotic genes. E2F1 also relocalizes and forms foci at sites of DNA double-strand breaks but the function of E2F1 at sites of damage is still unknown. Here I reveal that E2F1 deficiency …
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Quantitative measurement and modeling of the DNA damage signaling network : DNA double-strand breaks
DNA double-strand breaks (DSB) are one of the major mediators of chemotherapy-induced cytotoxicity in tumors. Cells that experience DNA damage can initiate a DNA damage-mediated cell-cycle arrest, attempt to repair the damage and, if successful, resume the cell-cycle (arrest/repair/resume). Cells …
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A novel DNA damage quantification platform enables high throughput screening for genes that impact DNA double strand breaks
DNA is the blueprint of life, and the high fidelity transmission of genetic information from parent cells to progeny is essential for an organism's viability. However, our genomes are constantly being damaged by reactive molecules generated from cellular metabolic processes or introduced from the …
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Proteomic Identification of Histone Post-Translational Modifications Induced By Dna Double-Strand Breaks and Novel Proteins Involved In The Dna Damage Response
<p>Inaccurate repair of DNA double-strand breaks (DSBs) can lead to DNA mutation and chromosome rearrangements, causing human diseases such as cancer. Although we know the basic mechanisms of DSB repair, the added complexities in the chromatin context are unclear. This is partially due to the lack …
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A novel high-throughput in-cell Western assay for the quantitative measurement of signaling dynamics in DNA damage signaling networks : cell decision processes in response to DNA double strand breaks
Following exposure to DNA damage, cells initiate a stress response involving multiple protein kinase signaling cascades. The DNA damage response results in one of several possible cell-fate decisions, or cellular responses: induction of cell-cycle arrest, initiation of DNA repair, activation of …
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Synthetic Lethality of Cdk Inhibition and Doxorubicin In Triple-Negative Breast Cancer Requires P53 Inactivation
… complete the cell cycle even in the presence of DNA damage. Therefore, we <strong>hypothesized that TNBC cells are sensitive to cell cycle targeted combination therapy, which leaves non-transformed cells unharmed</strong>. Our findings demonstrate that sequential administration of the pan-CDK …
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Genetic and Cytological Analysis of the Spindle Assembly Checkpoint Component BUB-3 in DNA Damage Response
The spindle assembly checkpoint and the DNA damage response have been studied for decades as independent pathways exerting separate functions. However, crosstalk between these cellular processes has emerged. The extent of overlap between these pathways is still unknown, especially in multicellular …
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The in Vivo Roles of the RdgB Enzyme in Escherichia Coli and the Detection of Rare Non-Canonical DNA Precursors
… make up the pool of canonical DNA precursors in living organisms. The incorporation of non-canonical DNA precursors into DNA during replication can lead to mutagenesis, chromosomal damage, or both. The approach taken by living organisms to address this problem is to have a set …
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Interrogating DNA replication stress and DNA damage responses via phenotypic and functional CRISPR/Cas9 screens
DNA double-strand breaks are believed to be one of the most cytotoxic DNA lesions, posing a constant threat for genome integrity and cell survival. Consequently, various DNA repair processes and pathways have evolved to deal with different DNA lesions and ensure genome stability. The tumour …
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STUDYING THE INTERACTION OF CANCER AND THROMBOSIS THERAPEUTICS WITH PROTEIN AND DNA
… of therapeutics. Many cancer drugs bind to DNA and inhibit replication. We have AFM and simulations, based on the worm-like chain model (WLC), to study DNA conformational changes due to its interaction with two cancer drugs, cisplatin and Pt-Acramtu. We found that cisplatin induces a 36º …
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The impact of age, exposure and genetics on homologous recombination at the engineered repeat sequence in mice
… is a critical pathway for the repair of DNA double-strand breaks and broken replication forks. Although homologous recombination is generally error-free, recombination between misaligned sequences can lead to deleterious sequence rearrangements, and conditions that stimulate homologous …
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A comparison of three gadolinium based approaches to cancer therapy
… increase cell killing when Gd binds to DNA. Two other methods induce Auger electrons in gadolinium isotopes: the beta decay of 159Gd, and K shell photoelectric events during photon irradiation. To determine the potential application that Auger cascades from these three processes could …
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The Extent and Nature Of Chromosomal Rearrangements in B Lymphocytes
… translocations, require formation and joining of DNA double strand breaks (DSBs). These events disrupt the integrity of the genome and are frequently involved in producing leukemias, lymphomas and sarcomas. Mature B cell lymphomas are unique among tumors in that they frequently carry clonal …
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THE FUNCTION OF MRN (MRE11-RAD50-NBS1) COMPLEX DURING WRN (WERNER) FACILITATED ATM (ATAXIA-TELANGIECTASIA MUTATED) ACTIVATION
… initiates a signaling pathway in response to DNA double strand breaks (DSBs). Genomic disorder ataxia-telangiectasia (A-T) is associated with defective ATM. WRN protein is involved in ATM pathway activation when cells are exposed to DSBs associated with replication fork collapse. Because the …
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Analysis of cell cycle surveillance mechanisms in meiosis
Numerous DNA double-strand breaks (DSBs) are introduced into the genome in the course of meiotic recombination. This poses a significant hazard to the genomic integrity of the cell. Studies in a number of organisms have unveiled the existence of surveillance mechanisms or checkpoints that couple …
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Regulation of neuronal genomic integrity through histone deacetylase cooperativity
… SIRT1 and HDAC1, in the neuronal response to DNA double strand breaks (DSBs). Both SIRT1 and HDAC1 were previously shown to promote neuronal survival in a mouse model of neurodegeneration in which the appearance of DSBs precedes other neurotoxic symptoms. Here I show for the first time the …
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Mechanisms of DNA double strand break-mediated neurotoxicity in neurodegenerative disease
Neurons are highly susceptible to DNA damage accumulation due to their large energy requirements, elevated transcriptional activity and long lifespan. DNA double strand breaks (DSBs) are also linked to neurodegeneration and senescence. However, it is not clear how DSB-bearing neurons influence …
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