Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 32 for “"DNA detection"”.
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Novel DNA probes for sensitive DNA detection
The ability to detect and interrogate DNA sequences allows further understanding and<br/>diagnosis of genetic disease. The ability to perform such analysis of genetic material<br/>requires highly selective and reliable technologies. Furthermore techniques which can use<br/>simple and cheap …
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Novel strategies for DNA detection and assay.
… was aimed at developing new strategies for DNA bioassays, using alternative detection techniques and new labelling methods. Firstly, a new labelling technique for DNA sequencing was developed using lanthanide chelates. These compounds were identified as likely candidates to replace …
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Development of DNA detection assays using functionalised nanoparticles
The ability to detect DNA relating to a specific disease is at the forefront of current research in molecular diagnostics. The detection techniques utilised for such work need to afford excellent selectivity as well as sensitivity. Current methods commonly involve the use of fluorescence; however …
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Novel enzymatic methods of DNA detection using enhanced Raman scattering
… have been a number of significant advances in DNA detection. In particular the advent of the Polymerase Chain Reaction (PCR) has enabled extremely low levels of DNA to be amplified and quantified, and along with fluorescence based detection measurements, has provided a reliable method of …
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Surface enhanced Raman scattering based assays for DNA detection by
… (SERS) based molecular diagnostic assays for the detection of specific DNA sequences have been developed in recent years to compete with the more common fluorescence based approaches. Current SERS assays require either time consuming separation steps that increase assay cost and can also increase …
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High sensitivity DNA detection using gold nanoparticles and conducting polymers
The detection and quantitation of specific nucleic acid (NA) sequences continues to grow in importance driven by issues ranging from personalized medicine to companion diagnostics such as antibiotic selection for infectious diseases. In order to enhance the sensitivity of electrochemical detection …
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Microgap Structured Optical Sensor for Fast Label-free DNA Detection
DNA detection technology has developed rapidly due to its extensive application in clinical diagnostics, bioengineering, environmental monitoring, and food science areas. Currently developed methods such as surface Plasmon resonance (SPR) methods, fluorescent dye labeled methods and electrochemical …
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DNA detection system of homo-oligomers by electrochemical impedence measurements and quantification through radiolabeling
… stranded homo-oligonucleotides was developed for DNA detection. Silanating agents (3-aminopropyltriethoxysilane, 3-glycodoxyethylpropylamine) were used to modify the electrodes and immobilize single stranded DNA. A method based on the electrochemical modification of Si substrates with bromobenzene …
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Methylated DNA detection using a high-affinity methyl-CpG binding protein and photopolymerization-based amplification
… many normal, cellular processes, aberrant DNA methylation has been implicated in the development of human diseases such as cancer. Medical research has begun to identify hypermethylated gene promoters that can be used as predictive, prognostic, and diagnostic biomarkers. Current …
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Human impact on the McMurdo Dry Valley soils of Antarctica: Extending the limits of DNA detection
… research quantified human impact, in the form of DNA released from humans and human-associated microbiota, across spatial and temporal scales. This project was part of the MBIE grant entitled “Evidence-based Risk Assessment of the McMurdo Dry Valley Ecosystem”. Areas of human activity, including …
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Exploration of cell-free DNA’s biological properties for better understanding and improved circulating tumour DNA detection
… that can be used for cancer profiling. Cell-free DNA (cfDNA) are fragmented DNA shed into circulation from various types of cells. The cfDNA derived from cancer cells, circulating tumour DNA (ctDNA), forms a subset of cfDNA. It can be challenging to detect ctDNA as it often constitutes only a …
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DNA diagnostic assays using Surface Enhanced Raman Scattering (SERS)
DNA is the prerequisite for all biological life and its discovery has revolutionised the understanding of biomolecular interactions and disease expression. This has enabled significant improvements in patient diagnosis and medical treatment to be carried out. The advancements in technology and …
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Studying DNA-Directed Nanoassembly, Hybridization and Mismatch-Discrimination via Plasmon Scattering of Gold Nanoparticles
… (nAu) and the specific recognition attributes of DNA to investigate the nAu-DNA assembly formation, and to apply the nanoassemblies for DNA detection. The nanoassembly structures, successfully correlated to the LSPR wavelength shift and the plasmon color change, showed that their homogeneous …
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Gene injection in the bovine: effect of time of microinjection and nuclear transfer technologies
… in vitro. Experiment 1 examined the effect of DNA microinjection at 11, 15 and 19 h after fertilization (haf) on survival rate and DNA detection frequency by polymerase chain reaction (PCR). There was no difference in transgene detection frequency between treatments (53% at 11; 50% at 15; 48% …
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Optical diffraction-based silicon sensors for the detection of DNA sequences
… sensors on silicon (100) substrates for the detection of complementary DNA sequences using colloidal gold labels. In-depth analysis of variations of DNA sequence within the human genome and association with diseases is expected to lead to personalised medical treatment. There is a great need …
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Rapid and portable molecular detection tools for reptile pest priority species in Australian environmental biosecurity scenarios
… Amplification (LAMP) as an emerging tool for the DNA detection of high-risk species in environmental biosecurity and compliance contexts. To help combat the trade of B. constrictor and P. guttatus I have developed fit for purpose LAMP assays for environmental biosecurity compliance. I rigorously …
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Nanopores for detecting and sensing biological molecules
In spite of significant advances in the detection, separation and counting of single biological molecules (DNA, proteins, aminoacids, etc.) with solid-state nanopores, atomically-resolved scanning and detection of these molecules remains a significant challenge. In most nanopore-based DNA …
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Development of 11-plex assay for the rapid screening of samples for detection of Shiga toxin-producing E. coli
… cytometry and multiplex microsphere arrays for detection of nucleic acid based signatures. By using MOL-PCR for detecting unique STEC DNA signatures in samples from the beef supply chain (farm to table) this assay will provide a multiplex and high throughput complement to the multiplex PCR …
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Ultrasensitive methods for the detection of biomolecular systems at the micro/nano scale
… to the development of new devices for specific DNA sequences detection, due to the relevance of nucleic acid analysis in many areas, including clinical diagnostics, environmental monitoring and food-quality control. However, diagnostic tools used for the detection of nucleic acids need to fulfil …
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Development of luminescent probes for ultrasensitive detection of biopolymers, their complexes, and living cells
… in major biological applications including the detection of nucleic acids and living cells. The first project used luminescent lanthanide ion probes that take advantage of hyper-sensitive acquisition of their long-lived emission signal in time- resolved mode, which avoids short-lived background …
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