Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 31 for “"DNA delivery"”.
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Mechanistic Study of DNA Delivery from Self-Assembled Nanolayered Films
The delivery of nucleic acids to modulate gene expression levels can enable highly specific and durable therapeutic effects. Formulation to protect nucleic acid cargoes and direct them to target tissues is critical to the success of these promising therapies. Delivery of nucleic acids, such as …
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Structure-Property-Transfection Relationships in Polycation-mediated Non-viral DNA Delivery
Non-viral gene delivery agents, such as cationic polyelectrolytes, are attractive replacements to viruses due to the absence of potential immunogenic risk and the ability to tune their macromolecular structure. Although non-viral vectors possess numerous design advantages, several investigators …
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Cationic Glycopolymers for DNA Delivery: Cellular Internalization Mechanisms and Biological Characterization
… the biological mechanisms of polymeric DNA delivery is essential to develop vehicles that perform optimally. In this work, the cellular internalization mechanisms of poly(glycoamidoamine) (PGAA) DNA delivery polymers were investigated. Polymer:DNA complexes interact with cell-surface …
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Entwicklung eines "DNA-Delivery"-Systems auf der Basis von Virulenz-attenuierten Listerien
… geeignete Vektoren für den Transport von Vakzine-DNA in das Zytosol von Antigen-präsentierenden Zellen ("DNA delivery"). In dieser Arbeit wurde dazu das intrazelluläre Bakterium Listeria monocytogenes verwendet, welches sich im Zytosol von Zellen vermehrt und fortbewegt. Ausgestattet mit einer …
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Biocompatible polymeric nanoparticles for drug/DNA delivery using biologically friendly polymerisation processes
This thesis explores the synthesis of several different polymeric nanostructures using reversible addition-fragmentation chain transfer (RAFT) polymerisation in both aqueous and ethanolic solutions.<br/><br/>A preface entailing an abstract, contents, common abbreviations and details of where this …
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Layer-by-layer surface manipulation and biointegration of quantum dots : assembly of nanostructured DNA delivery vehicles
Objectives: The aims of this investigation are to (i) prepare hybrid quantum dot (QD)-polymer compleses, (ii) maniplulate structural and chemical properties of the hybrids and characterize their effects on biocompatibility, and (iii) assemble diverse, heterostructured complexes for enhancing and …
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A comparative study of cationic formulations for the delivery of siRNA and DNA
… have been reports showing effective plasmid DNA delivery to cells and such systems could be used to deliver siRNA to cells due to the similarity of the delivery criteria between plasmid DNA and siRNA. Therefore, I hypothesise that a successful siRNA delivery system will have similar …
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Exploring new applications for photophysically encoded microcarriers
… cationic polysaccharides (PQ-4 and PQ-10) for DNA delivery. We have shown that, compared to PEI based polyplexes, they were less efficient in transfecting cells. However, as they had very low toxicity, further tailoring of the nature and extent of cationic side chains on cationic …
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Characterization of a transposon-induced pleiotropic metronidazole resistant mutant of Clostridium acetobutylicum P262
… strain, which lacks conventional systems for DNA delivery, was assessed, and the nature of metronidazole susceptibility in the C. acetobutylicum wild type was investigated. The metronidazole resistant transconjugant of interest, referred to as mutant 3R, was shown to harbour a single insertion …
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Syntheses, structures and properties of novel poly(amino ester)s
… and good transfection efficiency for DNA delivery comparable to polyethylenimine (PEI). Furthermore, some hyperbranched poly(amino ester)s can emit blue fluorescence. These poly(amino ester)s should be applicable for safe and efficient gene and drug delivery and as novel biocompatible …
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Imidazole-Containing Polymerized Ionic Liquids for Emerging Applications: From Gene Delivery to Thermoplastic Elastomers
… charge density and hydrogen bonding on nonviral DNA delivery. Copolymers with higher charge densities exhibited increased cytotoxicity, whereas increased hydroxyl concentrations remained nontoxic. DNA binding affinity increased with increased charge densities and increased hydroxyl content. …
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DEVELOPMENT OF TRAPPING STYLE CASSETTES FOR NEW GENE TARGETING STRATEGIES
… precise cell culture assay, and recombinant DNA delivery will also be important. The vector design work in this PhD thesis encompasses 0.00001 % ofthese efforts but may to out to be highly relevant...
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A Fast Assay of Bacteria Cell Permeability for Genetic Transformation
… of applications. One method of intracellular delivery of foreign DNA during the genetic engineering process is the use of electroporation to create pores along the bacterial cell membrane. Current methods for assessing pore formation do not directly measure cell permeabilization or enable …
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Characterization of nanoparticle-DNA conjugate and control of DNA conformation on particle surface
… hybridization and de-hybridization phenomena of DNA which are one of its fundamental functions. In particular, conjugates of gold nanoparticles and DNA (Au NP-DNA) have been extensively explored for their potential in biological applications such as DNA delivery for gene therapy and disease …
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Complexation Between DNA and Hydrophilic-Cationic Diblock Copolymers
DNA-polycation complexes (polyplexes) are gene delivery vehicles that offer a number of advantages over viral vehicles: high genetic payload capacity, low toxicity, and low cost. Among the many polycation architectures, hydrophilic-cationic diblock copolymers are particularly promising due to their …
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Understanding barriers to efficient nucleic acid delivery with bioresponsive block copolymers
The delivery of nucleic acids has the potential to revolutionize medicine by allowing previously untreatable diseases to be clinically addressed. Viral delivery systems have been held back by immunogenicity and toxicity concerns, but synthetic vectors have lagged in transfection efficiency. This …
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Cationic Polymers and Polymeric Micelles as Plasmid DNA and CRISPR-Cas9 Ribonucleoprotein Delivery Vehicles
… an alternative, holds great potential by direct delivery of genetic materials such as nucleic acids and genome editing machineries into human body to achieve long-term therapeutic protein expression, malfunctioning gene silencing, and native gene alternation. As a crucial step towards gene …
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Control of Inflammation Using Drug Delivery Strategies in in vitro Models
… mimic the binding site for the TF on the native DNA, but do not encode for any proteins. By binding to the TF in the cytoplasm, TFD have potential to limit excessive immune signaling and inflammatory protein production. Unfortunately, clinical success of TFD has been hampered by a lack of an …
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Mechanisms of Cytotoxicity and Intracellular Trafficking for Gene Delivery Polymers
… energy transfer (FRET) studies to monitor pDNA release from the polymer. Much of the work in this dissertation focuses on elucidating the intracellular mechanisms of linear poly(ethylenimine) (PEI) and how it compares to poly(L-tartaramidopentaethylenetetramine) (T4) and …
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