Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 32 for “"DNA damage checkpoint"”.
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THE DNA DAMAGE CHECKPOINT REGULATES MISMATCH REPAIR COMPLEXES DURING SINGLE STRAND ANNEALING PATHWAY
… The vast majority of lesions are single-strand DNA breaks caused by physiological processes such as DNA replication or oxidative stress during cellular metabolism. Moreover, single-strand DNA breaks can be converted to DNA double-strand breaks (DSBs), which are the most dangerous form of DNA …
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New Insights into the Roles of Human DNA Damage Checkpoint Protein ATR in the Regulation of Nucleotide Excision Repair and DNA Damage-Induced Cell Death
… threatened by endogenous and exogenous DNA damaging reagents that may lead to genome instability and cancer. Cells have evolved multiple mechanisms to repair DNA damage or to eliminate the damaged cells beyond repair and to prevent diverse diseases. Among these are ataxia telangiectasia …
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DNA stress checkpoint control in Arabidopsis thaliana by WEE1
… The same control mechanism is utilized by the DNA damage checkpoint to induce a cell cycle arrest upon DNA stress. In plants, a homolog of the WEE1 gene is present and is also responsible for phosphorylation of CDKs, although only during DNA damage. Plants that not contain a functional WEE1 …
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A functional role of chromatin remodeling factor, Rsf-1 in the DNA damage signaling pathway
… been reported that SNF2h ATPase is recruited to DNA damage sites (DSBs) in poly(ADP-ribosyl) polymerase 1 (PARP1)-dependent manner in DNA damage response (DDR), the function of Rsf-1 is still elusive. Here we show that Rsf-1 is recruited to DSBs confirmed by various cellular analyses …
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Cell cycle-dependent modification of Pot1 and its effects on telomere function
… telomerase access while suppressing a bona fide DNA damage response (DDR) at linear chromosome ends. However, the mechanisms that link cell cycle progression with telomere functions are largely unknown. Here we show that a key S-phase kinase, DDK (Dbf4-dependent protein kinase), phosphorylates …
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Zur Funktion des MPH1-Gens von Saccharomyces cerevisiae bei der rekombinativen Umgehung von replikationsarretierenden DNA-Schäden
… zeigen und sensitiv gegenüber verschiedenen DNA-schädigenden Agenzien sind, mit Mutanten aus verschiedenen DNA-Reparaturwegen konnte weitgehend ausgeschlossen werden, dass Mph1 zur Basen- und Nukleotidexcicionsreparatur, der transkriptionsgekoppelten Reparatur, dem nicht-homologen end joining …
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Proteomic Identification of Histone Post-Translational Modifications Induced By Dna Double-Strand Breaks and Novel Proteins Involved In The Dna Damage Response
<p>Inaccurate repair of DNA double-strand breaks (DSBs) can lead to DNA mutation and chromosome rearrangements, causing human diseases such as cancer. Although we know the basic mechanisms of DSB repair, the added complexities in the chromatin context are unclear. This is partially due to the lack …
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Integration of the phosphorylation-dependent signaling in the DNA damage response network : implications for cancer
The cellular response to DNA damage is an evolutionarily conserved process mediated by Ser/Thr kinases that results in the formation of multiple protein-protein complexes designed to control the cell cycle. The assembly of those multi-protein complexes is often triggered by the phosphorylation of a …
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Novel Role of CHK2 in the Intrinsic and Extrinsic Apoptosis Pathway
… response to genotoxic stress, cells activate DNA damage checkpoint pathways that maintain genetic fidelity, promote cell survival, and prevent malignant transformation. Checkpoint kinase 2 (CHK2) is an evolutionarily conserved serine/threonine protein kinase that is activated in response to …
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Investigating the role of NSMCE2 in cell cycle progression and genome maintenance
… is not due to the activation of the replication checkpoint, although the ligase-dead cell lines did activate the S phase DNA damage checkpoint. Based on what is already known about NSMCE2 function, it is suggested that the most likely cause of the proliferation defects is un-sensed, unresolved …
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DNA damage responses in the context of the cell division cycle
During my PhD, I have investigated aspects of the DNA damage response (DDR) in the context of three different cellular scenarios: DNA damage signalling in response to double-strand breaks during mitosis, coordination of DNA replication with DNA damage responses by regulation of the GINS complex, …
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Genetic interactions of repriming and translesion synthesis
… It has been previously shown to restart DNA synthesis past replication impediments such as DNA damage or non-B-DNA secondary structures. To probe the genetic interactions of PRIMPOL, genome-wide CRISPR/Cas9 knockout screens were undertaken in the human TK6 cell line. The most interesting …
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Harnessing mutagenic homologous recombination for in vivo targeted mutagenesis by TaGTEAM
… (TaGTEAM). By fusing the yeast 3-methyladenine DNA glycosylase MAG1 to a tetR DNA binding domain, we are able to elevate mutation rates > 800-fold in a specific ~20 kb region of the genome or on a plasmid that contains an array of tetO sites. A wide spectrum of transitions, transversions, and …
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Mathematical Modeling Reveals That G2/M Checkpoint Override
<p>Cell cycle checkpoints determine whether cells meet requirements to progress through the next stage. In response to DNA damage, how cells activate checkpoints have been well studied, but little is known about checkpoint deactivation (recovery), which directly impacts on cell fate. In tumor …
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Mathematical Modeling Reveals That G2/M Checkpoint Override Creates A Therapeutic Vulnerability In Tsc2-Null Tumors
<p>Cell cycle checkpoints determine whether cells meet requirements to progress through the next stage. In response to DNA damage, how cells activate checkpoints have been well studied, but little is known about checkpoint deactivation (recovery), which directly impacts on cell fate. In tumor …
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Mathematical Modeling Reveals That G2/M Checkpoint Override Creates A Therapeutic Vulnerability In Tsc2-Null Tumors
<p>Cell cycle checkpoints determine whether cells meet requirements to progress through the next stage. In response to DNA damage, how cells activate checkpoints have been well studied, but little is known about checkpoint deactivation (recovery), which directly impacts on cell fate. In tumor …
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Characterisation of non-essential genes required for survival under conditions of DNA stress
… to threats to the genome. This can lead to DNA replication stress and the potential loss of genetic integrity of the newly formed cells. The study of the sophisticated systems of DNA replication has been an attractive area for research in human and cancer, yet, the results do not fully …
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Prediction and Investigation of Novel Proteins in DNA Double Stranded Break Repair
DNA double stranded breaks (DSBs) are the most genotoxic forms of DNA lesions, causing fragmentation of the DNA strands. Mis-repaired and unrepaired DSBs lead to chromosomal rearrangement and genomic instability promoting tumorigenesis. DSBs are primarily repaired by two independent and highly …
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Meiotic Dna Re-Replication And The Recombination Checkpoint
… strict sequence of events, so that one round of DNA replication precedes programmed recombination and two nuclear divisions. Cyclin dependent kinase 1 (Cdk1) is required for meiosis, and any disruption in its activity leads to meiotic defects. The Cdk1 inhibitor, Sic1, regulates the G1-S …
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