Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 84 for “"DNA content"”.
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The Significance of Inter- and Intraspecific DNA Content Variation in Agriculturally Important Species
This dissertation examined the total nuclear DNA content variation in agriculturally important plant species and determined any differences. The nuclear DNA content of 40 Malus species and hybrids was examined using flow cytometry. Estimates of total nuclear DNA content for this collection ranged …
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Sympathetic Innervation of the Rat Adrenal Cortex and Its Effects on the Compensatory Adrenal Cortical Growth Response to Unilateral Adrenalectomy (Catecholamine, Endocrine, Splanchnic Nerve, Cell Proliferation)
… and not accompanied by the usual increase in DNA content. The effects of sympathectomy on compensatory adrenal growth were not attributable to suppression of the renin-angiotensin system because direct inhibition of angiotensin II and III synthesis with Enalapril (20 mg/kg orally) did not …
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Determining how variations in cell and nuclear size contribute to mitosis and tumorigenicity in cancer cells that undergo whole genome doubling
… and nuclear size, consistent with the change in DNA content, compared to the diploid (2N) cells from which they are derived. In cancer cells, however, cell and nuclear size do not always correlate with DNA content. This suggests that we do not understand how cell and nuclear size scale in …
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Adipocyte hyperplasia and fat deposition in high-weight and low- weight chickens
… concomitant increases in the total adipocyte DNA content of the assayed fat depots. Injections of DNA-specific [methyl³H] thymidine resulted in higher radioactivity levels in the fat depots of forced versus ad libitum or restricted-fed birds. Evidently, stimulation of fat deposition in adult …
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Effects of sodium hyaluronate and triamcinolone acetonide on proteoglycan metabolism in equine articular chondrocytes treated with interleukin-1
… assayed for newly synthesized GAG, total GAG content, total DNA content, and mRNA levels of collagen type II, aggrecan, and COX-2. The high concentration of HA (2.0 mg/mL) increased GAG synthesis while the high concentration of TA (0.6 mg/mL) decreased loss of GAG into the media. Both the high …
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Biochemical Characterization of Normal Navicular Bone Flexor Surface Cartilage
… from 8 horses 2 to 5 years old. Water, DNA, total collagen, total glycosaminoglycans, chondroitin sulphate, and keratan sulphate contents were determined. The results from each site were compared and the differences were analyzed by paired t-test (P < 0.05). Significant differences were …
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Chromosome pairing and its relationship to fertility in cytotypes of prairie cordgrass (Spartina pectinata Link)
… this potential effect, pollen morphology and DNA content of pollen nuclei were analyzed in tetraploids and hexaploids. Analysis of iii pollen morphology showed that micropollen was not formed as a result of abnormal meiosis I, suggesting that abnormal meiosis I may not lead to vast differences …
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Discovery of new biomarkers of mild cognitive impairment and Alzheimer's disease risk in buccal cells using laser scanning cytometry
… imaging and immunohistochemistry with high-content, multi-color fluorescence analysis, and can be used to identify specific cells in a heterogeneous population as well as scoring unique molecular events within them. This study aimed at investigating buccal cell types (buccal cell cytome) by …
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Understanding the weedy Chenopodium complex in the north central states
… species were investigated using morphological DNA content values and sequence data. With the exception of morphological characters such as pericarp covering the seeds, calyx shape (keeled or not) and DNA content of the species, most of the morphological characters were not phylogenetically …
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Isolation and effects of citrus limonoids on cytochrome p450 inhibition, apoptotic induction and cytotoxicity on human cancer cells.
… (nomilinic acid 17beta-D glucopyranoside), and DNAG (deacetylnomilinic acid 17beta-D glucopyranoside), have shown superoxide scavenging at millimolar levels. Micromolar amounts of LG and OG induced rapid necrosis of SH-SY5Y cells. Cytotoxicity was correlated (P = 0.046) to a concentration and …
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Evolutionary history of the canary grasses (Phalaris, Poaceae)
… based on the nuclear ITS and plastid trnT-F DNA regions allowing species relationships and the importance of polyploidy in speciation to be assessed. Divergence dates for the genus were determined using Bayesian methods (BEAST, version 1.6.2) and historic patterns of dispersal were analyzed …
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Extended mechanical stimulations of cartilage for growth and repair
… The protocol uses total glycosaminoglycan and DNA content assays to measure matrix macromolecule biosynthesis and uses L-³H-proline and ³⁵S-sulfate radiolabel incorporation rates to measure rates of biosynthesis. The results also demonstrate the feasibility of replacing the fetal bovine serum …
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The splicing inhibitor Isoginkgetin leads to defects in multiple phases of the cell cycle
… while the p53-null subline accumulated with 4C DNA content. Despite the pronounced difference in these isogenic strains, there was no pronounced p53 transcriptional response as assessed by qRT-PCR and preliminary microarray analysis. Instead, Isoginkgetin leads to altered pre-mRNA splicing of S …
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Chromosomes and spermatozoa of the great apes and man
… species have been analysed in relation to the content of highly repeated satellite DNAs in man and homologous sequences in the great apes. This has shown that the banding patterns are not informative about these sequences, and that they must reflect a degree of chromosome organization due to …
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Novel Biomarkers within Extracellular Vesicles for the Identification of Traumatic Brain Injury
… of EVs are also reflected in both mitochondrial DNA content, nuclear DNA content, and brain-derived cellular markers NFL, GFAP, and Iba1. Additionally, we combined a global mass spectrometry proteomics approach with biostatistical analysis and computational Graph Neural Network (GNN)-based …
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Use of monoploid solanum phureja in cell and tissue culture techniques for potato improvement
… of pollen based on propidium iodide staining of DNA. These populations corresponded to pollen separation based on size parameters alone, introducing the potential for flow sorting of pollen to increase seed set in 4x-2x crosses to tetraploid cultivars. Protoplast isolation from in vitro material …
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Evaluation of bacterial sampling methods for use with the bacterial tag-encoded flexible (FLX) amplicon pyrosequencing (bTEFAP) technique
… SKC PVC) were prescreened for low-background DNA content using Pyrosequencing. Studies comparing the performance of the SKC Polyvinylchloride (PVC) and SKC gelatin filters in IOM samplers to an impinger - the SKC biosampler - were conducted in a previously characterized bioaerosol chamber …
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Production of silver-loaded zeolites and investigation of their antimicrobial activity
… composition of fatty acid, as well as the DNA content of Escherichia coli K12W-T was studied. The results obtained showed silver ions appeared to elute from the zeolites frameworks into the TSB in anomalous trend. All three microorganisms were completely inhibited within one hour with the …
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Repetitive DNA sequences in maize: Possible roles in plant adaptation and transient gene expression
Fluctuations as high as 40% in the nuclear DNA content in maize have been observed as a result of adaptive response. The knob DNA sequence has been reported to contribute 16% of this variation. Other DNA sequences involved in genome size variation due to adaptive response have yet to be identified. …
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The role of the chromosomal passenger complex and condensin complex in meiotic chromosome cohesion and segregation
… goals. Among these, meiotic divisions reduce the DNA content of the cell, haploid gametes fuse and reenter mitotic cycling, and mitoses of early embryogenesis, in many animals, employ a rapid cell cycle that lacks gap phases. Much less is understood about regulation of these developmentally …
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