Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 34 for “"DNA cleavage"”.
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Mechanistic studies of bleomycin-mediated double-stranded DNA cleavage and structural studies of DNA containing normal and 4'-oxidized abasic sites
… of partial intercalation in double-stranded (ds) DNA cleavage mediated by a single bleomycin (BLM), a bulky group ([-cyclodextrin) was chemically attached to the polyamine tail of BLM A5 to prevent the intercalation of the bithiazole tail. The ability of this analog (CD-BLM) to effect ds-DNA …
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Dynamics of protein-DNA interactions in a Mu transpososome
… allows movement of a defined stretch of DNA, a transposon, from one DNA location to another. This process is required for the life-cycles of many viruses, from bacteriophage Mu to HIV; it is spreading antibiotic resistances between bacterial populations; and it is responsible for …
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CPISPR-CAS: From a Prokaryotic Immune System to a Gene Editing Tool
… I investigated the mechanism and function of RNA cleavage in type III CRISPR-Cas immunity. Secondly, I developed a tool to manipulate prokaryotic genomes and gene expression by using an engineered type II CRISPR-Cas system. To date, all three types of CRISPR-Cas systems target DNA. Type III …
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Protein Engineering via in Vitro Coevolution
… of homing endonuclease I-SceI with novel DNA cleavage activity. Homing endonucleases have great potential for applications such as targeted gene correction in gene therapy and gene alteration in systems biology and metabolic engineering. However, the limited repertoire of recognition …
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Melatonin's Protection against DNA Damage by the Iron (III)-Adriamycin Complex.
… rich. These features allow intercalation into DNA which, along with its topoisomerase inhibition, results in its anti-cancer effectiveness. Despite its success, its use is limited by a dose-dependent cardiotoxicity.</p> <p>Adriamycin forms tight complexes with iron in varying iron:drug ratios. …
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THE PROSURVIVAL ACTIVITIES OF APOPTOSIS-INDUCING FACTOR CONVERGE UPON THE REGULATION OF MITOCHONDRIAL METABOLISM, A NOVEL CELL DEATH PATHWAY, AND THE PROGRESSION OF PROSTATE CANCER
… apoptosis through chromatin condensation and DNA cleavage. Although AIF is recognized for its apoptotic function, AIF-mediated cell death has emerged as an atypical cell death pathway; and accumulating evidence indicates AIF to play a more significant role in cell survival by maintaining …
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DESIGN, SYNTHESIS, AND BIOLOGICAL EVALUATION OF NOVEL INDENOISOQUINOLINES AS POTENTIAL ANTICANCER AGENTS
… enzyme. These agents bind to a covalent Top1-DNA cleavage complex intermediate that is formed when Top1 relaxes supercoiled DNA. The effect of this stabilization is that a DNA replication fork can “collide” with the cleavage complex, which produces cytotoxic DNA damage products. Although the …
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The Transposon-Encoded Protein TnpB is an RNA-Guided Nuclease
… uses a noncoding RNA as a guide to cleave DNA substrates in a targeted manner. We investigate the biogenesis of the guide RNA, finding that TnpB can process its own mRNA to yield the guide RNA. In the process of studying guide RNA biogenesis, we also identify a potential cis-regulatory …
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Organization and dynamics of the bacteriophage Mu transpososome
… a set of spatially and temporally coordinated DNA phosphoryl transfer reactions to move the element from an existing DNA location to a new DNA site. The Mu transpososome, where the DNA cleavage and joining reactions that compose transposition take place, is a large nucleoprotein complex …
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Nucleobase-modified deoxyribozymes for amide and peptide hydrolysis
… into complex structures to enable catalysis. DNA is primarily double-stranded and is non-catalytic in nature. However, considering the structural similarity to RNA, single-stranded DNA should also be able to form complex structures and perform catalysis. In fact, artificial DNA enzymes …
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Large-Scale Applications of Multi-Reference Methods in Chemistry and Development of a Multi-Reference Moller-Plesset Perturbation Theory Program
… based on the formation of diradicals and causes DNA cleavage and finally cytolysis. Computations on model systems performed in the present work show the influence of structural features on the mode of action and the efficacy of this antitumor-antibiotic. The cyclization of systems related to the …
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The Interrogation of Cas9 Aptamers and sgRNA Structures Through SELEX
… variants that bind S. pyogenes Cas9 and support DNA cleavage. These variants demonstrate surprising malleability in the sgRNA sequence and are utilized in a combinatorial approach to identify scaffolds that enhance editing efficiencies when paired DNA-binding antisense domains. Using molecular …
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Staphylococcus aureus DNA gyrase: mechanism and drug targeting
… the development of antibiotic resistance. DNA gyrase is an essential bacterial type II DNA topoisomerase that manipulates DNA topology by performing transient double-strand breaks and DNA strand passage. As gyrase is vital for bacterial survival, it is an effective antibacterial target. By …
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Synthesis and evaluation of novel azaindolocarbazole derivatives as cancer chemotherapeutics
… COMPARE analysis, and subsequent topo I-mediated DNA cleavage assays showed inhibitory activity below 1 μM for several derivatives.
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Interrogation of CRISPR-Cas targeting specificity for mammalian genome engineering
Class II CRISPR-Cas RNA programmable DNA endonucleases enable high efficiency genome editing across the biological diversity for research, industrial, and biomedical applications. Human genome editing with CRISPR-Cas just recently made its debut in human clinical trials and has immense therapeutic …
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DNA target site recognition by the Ll.LtrB group II intron RNP
… retroelements that site-specifically insert into DNA target sequences. The group II intron mobility pathway is mediated by a ribonucleoprotein particle (RNP) composed of excised intron RNA and an intron-encoded protein (IEP). The intron lariat inserts at a specific DNA target sequence and is then …
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In Vivo Genome Editing: Proof of Concept in Neonatal and Adult Mouse Liver
… are engineered proteins capable of site-directed DNA cleavage, known to increase the efficiency of gene targeting. Our lab has previously demonstrated in vivo gene targeting of the liver using AAV to deliver ZFNs and therapeutic donor in neonatal mice, where the rapid proliferation of hepatocytes …
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Generation and analysis of transgenetic mice expressing catalytically inactive recombination activating gene-1
… metal ions required for catalysis of DNA cleavage. Mutation of any of these residues abrogates V(D)J recombination without affecting the DNA binding activity of the RAG protein complex. Accordingly, we have speculated that ectopic expression of a catalytically inactive form of RAG1 in …
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Deoxyribonucleic acid restriction/modification systems and gene transfer strategies in Ruminococcus albus 8 and Ruminococcus flavefaciens FD-1
One goal critical to the use of recombinant DNA technologies with rumen bacteria is the establishment of a stable DNA transfer system. Research to date has shown that Ruminococcus can be classified as a genus resistant to transformation, and that electroporation may offer the only means to …
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Reactions of graphene oxide and buckminsterfullerene in the aquatic environment
… anion (O2˙–)and hydrogen peroxide (H 2O2). DNA cleavage was observed in air-satured GO suspensions that contained NADH, suggesting that ROS production could be a mechanism for DNA damage by GO within biological cells. Indirect photochemical reactions involving GO were shown to occur in an …
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