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Showing 1 to 6 of 6 for “"DNA capture"”.
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Graphene supported hafnium oxide nanopores for DNA sensing
… fronts including surface charge engineering for DNA capture and conductance modulation in nanopores, nanowire transistors for localized detection, ultra-thin membrane fabrication using graphene, and the exploration of alternative nanopore materials for biosensing applications. In this work, the …
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Methods for Improving Challenging DNA Profiles and Molecular Preservation of Soft Tissue Samples
Degradation of DNA can lead to either poor quality, imbalanced, or even no profiles. Therefore, appropriate collection and storage methods are critical to minimize its impact. If the DNA is degraded prior to sample collection, then the degradation process can only be arrested and other methods have …
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Sample Collection and DNA Extraction Methods for Environmental DNA Metabarcoding in Headwater Streams
<p>DNA is found to be free and ubiquitous in the environment where it is no longer associated with the source organism, and is also known as environmental-DNA (eDNA). Methods optimized for specific environments may be able supplement insight to local taxa richness. With the advent of high …
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Selective Enrichment Through Capture (SEC): A Technology for Retrieving Specific DNA Sequences from Complex Mixtures
The capacity to selectively target DNA sequences within a complex mixture is a useful feature for genomic studies. Several methods aimed at undertaking this feat have utilized biotinylated oligonucleotides and streptavidin beads to capture DNA but have low efficiencies, require PCR, or cannot be …
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Biomolecular transport at and through two-dimensional materials
… recording the ionic current as a single-stranded DNA molecule is electrophoretically driven through a nanopore; ionic current blockades unique to each nucleotide provide a key to the sequence readout. 2D materials provide the ultimate resolution by isolating one or two nucleotides in the nanopore …
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Studio in vitro dell'espressione di varianti del gene CFTR con ruolo patofisiologico non ancora definito
… covering the full-length CFTR to be used for DNA capture (custom capture kit, Nimblegen SeqCap EZ Library, Roche). The application of NGS will lead to the improvement of time and cost of molecular diagnosis, that is nowadays increasingly relevant thanks to the recent advances obtained in …